Two-phase temporary immersion system for Agrobacterium rhizogenes genetic transformation of sage (Salvia tomentosa Mill.)

Marchev, Andrey S.; Georgiev, Vasil; Ivanov, Ivan; Badjakov, Ilian; Pavlov, Atanas

doi:10.1007/s10529-011-0625-5

Cited by 42 publications

(29 citation statements)

References 18 publications

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“…This system was therefore adopted for selecting putatively transformed embryos, as an alternative to the conventional method of selection on semi-solid medium. Although the use of TIS for mass clonal propagation has increased (Etienne and Berthouly 2002;Watt 2012), reports of its application for the selection phase of genetic transformation studies are scarce and involve genetic transformation based on adventitious shoot regeneration in pineapple (Espinosa et al 2002) and strawberry (Hanhineva and Kärenlampi 2007), or adventitious root formation in sage (Marchev et al 2011). However, none of these studies compared the transformation efficiency of TIS and semi-solid medium.…”

Section: Discussionmentioning

confidence: 99%

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Mallón

Viéitez

Vidal

2013

Plant Cell Tiss Organ Cult

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As the limiting factor for recovery of plants from oak embryogenic lines is the low embryo conversion rate, axillary shoot lines were established from transformed germinated embryos.Transformed embryos and shoots were cultured in medium with or without kanamycin and the responses to several morphogenetic processes (recovery after cryopreservation, germination, shoot proliferation, and rooting) were evaluated.2

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Section: Discussionmentioning

confidence: 99%

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Mallón

Viéitez

Vidal

2013

Plant Cell Tiss Organ Cult

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“…by Agrobacterium transformation and cultivation in two-phase temporary immersion RITA system with a presence of adsorbent resin Amberlite XAD-4 as a second phase. This new transformation protocol was proved to be very effective and could be widely applied in other plant species, overproducing and releasing phytotoxic phenolics in high concentrations [44]. The authors [44] established that about 85% of released phenolic acids and 100% of released flavonoids were removed from the medium and 100% of the explants formed strong and healthy hairy roots for two weeks.…”

Section: Differentiated In Vitro Systemsmentioning

confidence: 98%

“…Marchev and co-workers, 2011 [44] developed hairy root culture of S. tomentosa Mill. by Agrobacterium transformation and cultivation in two-phase temporary immersion RITA system with a presence of adsorbent resin Amberlite XAD-4 as a second phase.…”

Section: Differentiated In Vitro Systemsmentioning

confidence: 99%

Food additives and bioactive substances from in vitro systems of edible plants from the Balkan peninsula

Vrancheva

Ivanov

Aneva

et al. 2018

Engineering in Life Sciences

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During the last few years there is an increasing demand to the natural biologically active compounds. According to the World Health Organization (WHO) about 11% of the conventional medicines are of plant origin. Nowadays, plant biotechnologies are modern and reliable tool for producing valuable bioactive compounds. Recently, the potential of plant cells as foods also was confirmed. The advantages of plant in vitro systems over the intact plants are well known: growing under controlled and optimized laboratory conditions; independence of climatic and soil differences; preservation of rare and endangered plant species; cultivation in diverse bioreactor systems for increasing production yields of target metabolites. There have been developed many in vitro systems for production of various plant bioactive compounds with potential application in food industries. But potential for industrial implementation of this technology depends on solving problems with the scale‐up of bioreactor cultivation, development of additional approaches for improving/modification of bioactivities of the target plant secondary metabolites, and to find way to exclude or replace in the culture media the carcinogenic plant growth regulator 2,4‐dichlorophenoxyacetic acid (2,4‐D) with its safety analogs, such as α‐naphtaleneacetic acid (NAA) and/or indole‐3‐butyric acid (IBA). The aim of the current mini review is to summarize information about different in vitro systems of edible plants from the Balkan Peninsula with potential for producing food additives and biologically active substances and to describe prospects for successful industrial implementation of this technology.

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“…For separation of quercetin glycosides rutin and hyperoside a mobile phase of 2% (v/v) acetic acid (solvent A) and acetonitrile (solvent B) was used. The elution program for separation of phenolic acids, flavonoids and quercetin glycosides was described previously by Marchev et al (2011).…”

Section: Hplc Assaysmentioning

confidence: 99%

Polyphenol profile and antioxidant activity of extracts from olive leaves

Yancheva¹,

Mavromatis²,

Georgieva³

2016

j. cent. eur. agric.

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The compounds, derivatives of olive leaves have a high antioxidant activity. The content of the total phenolic compounds (TPC), antioxidant activity (AOA) and HPLC polyphenol profile of methanol extracts from the leaves of the olive cultivars Chondrolia Halkidiki, Kalamon, Koroneiki grown in the nursery (in vivo) and in vitro plants of Chondrolia Halkidiki were compared. The results obtained for TPC varied between 9.2±0.5 mgGAE*gDW -1 and 16.4±0.5 mgGAE*gDW -1 . Antioxidant capacity was determined by four methods DPPH, ABTS, FRAP and CUPRAC. The highest results for TPC and AOA were achieved for the leaves of Chondrolia Halkidiki grown in vitro. A high correlation between the results gained from the TPC and AOA was established. Conducted HPLC analysis revealed the presence of 3,4-dihydroxybenzoic, caffeic, sinapic and ferulic acids and quercetin, hesperidin and luteolin and the quercetin glycosides rutin and hyperoside.

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Two-phase temporary immersion system for Agrobacterium rhizogenes genetic transformation of sage (Salvia tomentosa Mill.)

Cited by 42 publications

References 18 publications

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Food additives and bioactive substances from in vitro systems of edible plants from the Balkan peninsula

Polyphenol profile and antioxidant activity of extracts from olive leaves

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