Characteristics of enzyme hydrolysis of cellulose under static condition

Taneda, Daisuke; Ueno, Yoshiki; Ikeo, Makoto; Okino, Shohei

doi:10.1016/j.biortech.2012.06.104

Cited by 38 publications

(28 citation statements)

References 30 publications

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“…This corroborates Buswell et al [28], who reported 30˚C as the optimum temperature for the production of cellulolytic enzyme complex by Trichoderma spp. This was also observed by Chen and Jan [29], Dalsenter et al [30], Mitchell et al [31], Gomes et al [32], Carvalho et al [33] and Taneda et al [34].…”

Section: Resultssupporting

confidence: 73%

Cellulase Production by Endophytic Strains of Trichoderma reesei from Baccharis dracunculifolia D. C. (Asteraceae)

Onofre¹,

Bonfante²,

Santos³

et al. 2014

AiM

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Cellulases are enzymes responsible for the degradation of cellulose, the major compound in plant cells. Cellulose is a polysaccharide composed of several glucose units linked together by chemical bonds. Cellulases, such as endoglucanases, beta-glucosidase and exoglucanases, break the chemical bonds between the glucose units. Fungi, including the endophytic species, can be great cellulase producers. This study aimed to evaluate cellulase production by four endophytic strains of Trichoderma reesei in semi-solid media containing sugarcane bagasse, supplemented or not with salts. Two fermentations were carried out for 43 days. Samples were taken every seven days to obtain production peaks. The enzymes were characterized by their optimum pH and temperature of activity and stability upon incubation in the presence of ions, pH and temperature variations. The results showed that the endophytic strains FB1, FB2, FB3 and FB4 of Trichoderma reesei produce cellulases in a sugarcane bagasse medium, supplemented or not with salts, at pH 5.5 and 30˚C. The supplemented medium proved to be more appropriate to induce cellulase production after 29 days of fermentation, with FB4 having the best yield: 16.32 ± 2.65 IU/gram of fermented substrate.

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Section: Resultssupporting

confidence: 73%

Cellulase Production by Endophytic Strains of Trichoderma reesei from Baccharis dracunculifolia D. C. (Asteraceae)

Onofre¹,

Bonfante²,

Santos³

et al. 2014

AiM

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“…Moreover, the improved mixing in trial 5 would also be expected to enhance enzyme distribution, and operation at a lower rpm, would probably decrease the shear stress imposed on the enzymes, which could otherwise change their three-dimensional structure and adversely affect their performance. 34,35 Figure 3 shows the torque profile for trial 5 during the second phase of fiber addition. The peak torque observed after 25.5 h was due to a brief increase in rotational speed from 60 rpm to 100 rpm, which was maintained for 1 h to assist in fiber blending.…”

Section: Impact Of Moderate Rotational Speeds On Mixing Of the Slurrymentioning

confidence: 99%

The Effect of Fed-Batch Operation and Rotational Speed on High-Solids Enzymatic Hydrolysis of Hardwood Substrates

GaonaAdriana

LawryshynYuri

SavilleBradley

2015

Industrial Biotechnology

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Enzymatic hydrolysis of hardwood substrates yields soluble sugars, which are a carbon source for producing ethanol and butanol. However, as the substrate and water are mixed, a viscous, heterogeneous slurry forms. The result is reduced mixing performance, mass transfer, and enzyme-substrate contact, all of which negatively affect sugar titers. In this study, enzymatic hydrolysis of a hardwood substrate at 20 wt% was conducted in a 10L stirred tank reactor. We investigated the dynamic changes in slurry behavior, as well as the interactions among mixer design/ operation and fed-batch strategies. The effects of the number and frequency of substrate additions, impeller configuration, and high and moderate agitation speeds were evaluated using torque measurements and in terms of glucan-to-glucose conversion. Fewer additions corresponds to batch operation and negatively impacted solids distribution, accurate torque readings, and glucan conversion. However, as the number of additions incrementally increased, glucan conversion increased, torque readings captured the resistance of the fluid to flow, and the solids distribution within the reactor improved. Moderate agitation speeds of 60 revolutions per minute (rpm), combined with smaller and more frequent fiber additions, led to increased glucan conversion and less torque resistance compared to similar experiments conducted at high rotational speeds (150 rpm), and the use of different impeller sizes and configurations impacted the distribution of the slurry. This work illustrates that the implementation of different fed-batch additions coupled with different impeller types and configurations promotes hardwood slurry blending, accommodates the increase in viscosity, and enables quantification of energy and power consumption.

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“…The reactor engineering route consists in using reactors with different shaking and/or mixing patterns (Ingesson et al, 2001;Roche et al, 2009;Kinnarinen et al, 2012;Lavenson et al, 2014), semi-batch reactors (Gupta et al, 2012), recycling enzymes (Xue et al, 2012), loading enzymes under static and agitated conditions http://dx.doi.org/10.1016/j.biortech.2014.09.088 0960-8524/Ó 2014 Elsevier Ltd. All rights reserved. (Taneda et al, 2012) and process integration (Lennartsson et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…(Chakraborty et al, 2010);(c) what is the optimal reactor mixing strategy for batch hydrolysis of cellulose? (Pal and Chakraborty, 2013);(d) what are the optimal mixing and temperature conditions for catalytic conversion of cellulose to biofuels in ionic liquid media? (Gaikwad and Chakraborty, 2014).…”

Section: Introductionmentioning

confidence: 99%

A multiscale three-zone reactive mixing model for engineering a scale separation in enzymatic hydrolysis of cellulose

Chakraborty

Raju

Pal

2014

Bioresource Technology

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Characteristics of enzyme hydrolysis of cellulose under static condition

Cited by 38 publications

References 30 publications

Cellulase Production by Endophytic Strains of <i>Trichoderma reesei</i> from <i>Baccharis dracunculifolia</i> D. C. (Asteraceae)

Cellulase Production by Endophytic Strains of <i>Trichoderma reesei</i> from <i>Baccharis dracunculifolia</i> D. C. (Asteraceae)

The Effect of Fed-Batch Operation and Rotational Speed on High-Solids Enzymatic Hydrolysis of Hardwood Substrates

A multiscale three-zone reactive mixing model for engineering a scale separation in enzymatic hydrolysis of cellulose

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Characteristics of enzyme hydrolysis of cellulose under static condition

Cited by 38 publications

References 30 publications

Cellulase Production by Endophytic Strains of &lt;i&gt;Trichoderma reesei&lt;/i&gt; from &lt;i&gt;Baccharis dracunculifolia&lt;/i&gt; D. C. (Asteraceae)

Cellulase Production by Endophytic Strains of &lt;i&gt;Trichoderma reesei&lt;/i&gt; from &lt;i&gt;Baccharis dracunculifolia&lt;/i&gt; D. C. (Asteraceae)

The Effect of Fed-Batch Operation and Rotational Speed on High-Solids Enzymatic Hydrolysis of Hardwood Substrates

A multiscale three-zone reactive mixing model for engineering a scale separation in enzymatic hydrolysis of cellulose

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Product

Resources

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Cellulase Production by Endophytic Strains of <i>Trichoderma reesei</i> from <i>Baccharis dracunculifolia</i> D. C. (Asteraceae)

Cellulase Production by Endophytic Strains of <i>Trichoderma reesei</i> from <i>Baccharis dracunculifolia</i> D. C. (Asteraceae)