Characterisation of novel Fusarium graminearum microsatellite markers in different Fusarium species from various countries

Vogelgsang, Susanne; Widmer, Franco; Jenny, Eveline; Enkerli, J.

doi:10.1007/s10658-008-9379-x

Cited by 23 publications

(19 citation statements)

References 16 publications

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“…Beauveria Clade C, for which they were not designed. These data correspond to those of previous studies reporting limited cross taxa amplification of SSR markers in fungi as compared to animals and plants ( Barbara et al, 2007;Vogelgsang et al, 2009). Similar to this study, loci that could be amplified across taxa showed no or only low levels of polymorphism suggesting that transfer of SSR markers among fungal species may generally be limited.…”

Section: Discussionsupporting

confidence: 91%

Diversity of Beauveria spp. isolates from pollen beetles Meligethes aeneus in Switzerland

Meyling

Pilz

Keller

et al. 2012

Journal of Invertebrate Pathology

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Section: Discussionsupporting

confidence: 91%

Diversity of Beauveria spp. isolates from pollen beetles Meligethes aeneus in Switzerland

Meyling

Pilz

Keller

et al. 2012

Journal of Invertebrate Pathology

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“…Several molecular markers have been used to analyse the diversity of Fusarium plant pathogens at the genome level: amplified fragment length polymorphism (AFLP) in F. graminearum and F. asiaticum O'Donell, Aoki, Kistler & Geiser (Qu et al 2008), random amplified polymorphic DNA (RAPD) in F. graminearum and F. poae (Ouellet and Seifert 1993;Kerényi et al 1997), simple sequence repeats (SSR) in F. graminearum and F. culmorum (Vogelgsang et al 2009), and inter-simple sequence repeats (ISSR) in F. graminearum and F. culmorum (Mishra et al 2003(Mishra et al , 2004. AFLP analysis has been used also for studying relationships at taxonomic levels within a world-wide collection of F. sporotrichioides Sherbakoff, F. langsethiae Torp & Nirenberg and F. poae isolates (Schmidt et al 2004).…”

Section: Introductionmentioning

confidence: 99%

ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England

et al. 2010

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Fusarium poae is one of the Fusarium species isolated from cereal grains infected by Fusarium head blight (FHB), and in recent years it has been identified as a major FHB component. In this study, 97 F. poae isolates from Argentina (n=62) and England (n=35) were analysed by inter-simple sequence repeats (ISSR) to examine the genetic diversity and to determine whether intraspecific variation could be correlated with geographic and/or host origin. The molecular analysis showed high intraspecific variability within F. poae isolates, but did not reveal a clear relationship between variability and the host/geographic origin. Fusarium poae isolates from the same geographic region or host appeared in different subclusters. Conversely, isolates with the same haplotype were also collected from different geographic regions. However, we did observe subclusters consisting of isolates from Argentina only or from England only. Furthermore, a single seed sample was found to host different haplotypes. Analysis of molecular variance (AMOVA) indicated a high genetic variability in F. poae, with most of the genetic variability explained by differences within, rather than between Argentinean and English populations. This is the first report on genetic diversity of F. poae using ISSR markers. Moreover, ISSR fingerprinting generates highly polymorphic markers for F. poae and proved to be a useful and reliable assay for genetic variability studies.

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“…The ability of the selected primers to produce species‐specific fingerprints was apparent. Fifteen novel microsatellite markers were isolated from Fusarium graminearum (Vogelgsang et al . 2009).…”

Section: Resultsmentioning

confidence: 99%

DEVELOPMENT OF DI‐ AND TETRANUCLEOTIDE REPEAT PRIMER FOR DISCRIMINATION OF FUSARIUM SPECIES

Abd-Elsalam

Bahkali

Moslem

et al. 2010

Journal of Food Safety

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The genetic diversity profiles of 19 isolates of the fungal pathogen Fusarium species, 14 from Egypt and 5 from Germany, were analyzed based on morphological characteristics and microsatellite markers. Five microsatellites were selected and primers were designed. Microsatellite‐primed polymerase chain reaction using the dinucleotide and tetranucleotide primers showed clear polymorphisms among the different Fusarium spp. isolates. Both primers gave similar results in phenetic analysis of genetic similarity between populations. Between Fusarium spp. isolates, similarities ranged from 38 to 62% for interspecific and 62 to 94% for intraspecific comparisons. Two major groups were observed in the dendrogram, which was divided into three subgroups. One of them consisted the five F.oxysporum f. sp. vasinfectum isolates at the genetic similarity of 92%. Isolates Fov1, Fov3 and Fov5 showed high genetic relatedness (100%). With the second main cluster, at 62% similarity, one subcluster could be discerned; the subcluster contained F. oxysporum, F. solani, F. sambucinum, F. poae and Fusarium spp. isolates. These experiments reveal that microsatellite primers are reliable, sensitive and technically simple tools for assaying genetic variability in Fusaria. PRACTICAL APPLICATIONS The results presented herein indicate that a microsatellite technique provides an efficient tool for the identification of polymorphic loci that can be used to monitor the genetic differences between Fusarium species. Upcoming research is warranted to develop more microsatellite primers with a wider array of Fusaria.

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Characterisation of novel Fusarium graminearum microsatellite markers in different Fusarium species from various countries

Abstract: Fifteen novel microsatellite markers were isolated from Fusarium graminearum.

Cited by 23 publications

References 16 publications

Diversity of Beauveria spp. isolates from pollen beetles Meligethes aeneus in Switzerland

Diversity of Beauveria spp. isolates from pollen beetles Meligethes aeneus in Switzerland

ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England

DEVELOPMENT OF DI‐ AND TETRANUCLEOTIDE REPEAT PRIMER FOR DISCRIMINATION OF FUSARIUM SPECIES

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