DEVELOPMENT OF DI‐ AND TETRANUCLEOTIDE REPEAT PRIMER FOR DISCRIMINATION OF <i>FUSARIUM</i> SPECIES

Abd-Elsalam, Kamel A.; Bahkali, Ali H.; Moslem, Mohamed A.; Verreet, Joseph‐Alexander

doi:10.1111/j.1745-4565.2010.00262.x

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…Genotypic diversity and polymorphism between isolates of different Fusarium species causing fruit rot disease in banana were assessed using six well‐established microsatellite primers for Fusarium species selected from latest previous study by Abd‐Elsalam et al . () and Bahkali et al . ().…”

Section: Discussionmentioning

confidence: 87%

“…There are six primers used in the microsatellite analysis, namely (AG)7C (AGAGAGAGAGAGAGC), (CA)7T (CACACACACACACAT), (CTG)5 (CTGCTGCTGCTGCTG), (TCC)5 (TCCTCCTCCTCCTCC), (TTTC)4 (TTTCTTTCTTTCTTTC) and (TAGG)4 (TAGGTAGGTAGGTAGG), followed by respective amplification protocol by Abd‐Elsalam et al . () and Bahkali et al . ().…”

Section: Methodsmentioning

confidence: 87%

“…Target DNA fragment band according to primers used with sizes ranging from 200 to 2000 bp (Abd‐Elsalam et al . ; Bahkali et al . ) was observed.…”

Section: Methodsmentioning

confidence: 99%

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Genetic diversity and pathogenicity of Fusarium species associated with fruit rot disease in banana across Peninsular Malaysia

et al. 2017

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Section: Discussionmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 87%

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Genetic diversity and pathogenicity of Fusarium species associated with fruit rot disease in banana across Peninsular Malaysia

et al. 2017

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Toxigenic profiles and trinucleotide repeat diversity ofFusariumspecies isolated from banana fruits

Alghuthaymi

Bahkali

2015

Biotechnology & Biotechnological Equipment

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Infesting Fusarium species isolated from banana fruit samples were identified and quantified by morphological, mycotoxicological and molecular tools. A total of 19 Fusarium isolates were obtained: F. semitectum was most predominant (26%), followed by F. proliferatum (16%), F. circinatum (16%), F. chlamydosporum (10.5%), F. solani (10.5%), F. oxysporum (10.5%) and F. thapsinum (5%). Fumonisin B1, deoxynivalenol and zearalenone contents were assayed by high-performance liquid chromatography (HPLC). Seventeen isolates, belonging to F. chlamydosporum, F. circinatum, F. semitectum, F. solani, F. thapsinum, F. proliferatum and Fusarium spp., produced mycotoxins when cultured on rice medium. Fumonisin was produced by all of the studied Fusarium isolates, except F. oxysporum, at a concentration of over 1 μg/mL. F. citrinium isolates 4 and 5 and F. solani isolate 3 were the most potent producers of deoxynivalenol. We compared the 19 Fusarium isolates based on the bands amplified by 10 microsatellite primers. Of these, seven primers, (TCC)5, (TGG)5, (GTA)5, (ATG)5, (TAC)5, (TGC)5 and (TGT)5, yielded a high number of bands and different mean number of alleles. The similarity level between isolates was calculated using a simple matching coefficient. Dendrograms were constructed by the unweighted pair-group method with arithmetical averages (UPGMA). Two main clusters were observed. The interspecific genetic similarity between Fusarium spp. isolates was between 40% and 58% and the intraspecific similarity from 58% to 100%, indicating a high degree of genetic diversity in the tested isolates. Some unexpected genetic similarities were observed among the isolates, indicating non-agreement between morphological and molecular identification of the isolates.

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Characterization of Novel Di-, Tri-, and Tetranucleotide Microsatellite Primers Suitable for Genotyping Various Plant Pathogenic Fungi with Special Emphasis on Fusaria and Mycospherella graminicola

Bahkali

Abd-Elsalam

Guo

et al. 2012

IJMS

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The goals of this investigation were to identify and evaluate the use of polymorphic microsatellite marker (PMM) analysis for molecular typing of seventeen plant pathogenic fungi. Primers for di-, tri-, and tetranucleotide loci were designed directly from the recently published genomic sequence of Mycospherlla graminicola and Fusarium graminearum. A total of 20 new microsatellite primers as easy-to-score markers were developed. Microsatellite primer PCR (MP-PCR) yielded highly reproducible and complex genomic fingerprints, with several bands ranging in size from 200 to 3000 bp. Of the 20 primers tested, only (TAGG)4, (TCC)5 and (CA)7T produced a high number of polymorphic bands from either F. graminearum or F. culmorum. (ATG)5 led to successful amplifications in M. graminicola isolates collected from Germany. Percentage of polymorphic bands among Fusarium species ranged from 9 to 100%. Cluster analysis of banding patterns of the isolates corresponded well to the established species delineations based on morphology and other methods of phylogenetic analysis. The current research demonstrates that the newly designed microsatellite primers are reliable, sensitive and technically simple tools for assaying genetic variability in plant pathogenic fungi.

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DEVELOPMENT OF DI‐ AND TETRANUCLEOTIDE REPEAT PRIMER FOR DISCRIMINATION OF FUSARIUM SPECIES

Cited by 4 publications

References 34 publications

Genetic diversity and pathogenicity of Fusarium species associated with fruit rot disease in banana across Peninsular Malaysia

Genetic diversity and pathogenicity of Fusarium species associated with fruit rot disease in banana across Peninsular Malaysia

Toxigenic profiles and trinucleotide repeat diversity ofFusariumspecies isolated from banana fruits

Characterization of Novel Di-, Tri-, and Tetranucleotide Microsatellite Primers Suitable for Genotyping Various Plant Pathogenic Fungi with Special Emphasis on Fusaria and Mycospherella graminicola

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