Changes in the Activity of Catalase (EC 1.11.1.6) in Relation to the Dormancy of Grapevine (<i>Vitis vinifera</i> L.) Buds

Nir, G.; Shulman, Y.; Fanberstein, L.; Lavee, S.

doi:10.1104/pp.81.4.1140

Cited by 82 publications

(48 citation statements)

References 17 publications

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“…In group RD, sequences related to metabolism and oxidation-reduction were the most abundant, which fits well with the idea that some basic metabolic activation is required to restart growth and cell division following dormancy and with S1 S2 S3 S4 S5 Z1 Z2 Z3 Z4 S1 S2 S3 S4 S5 Z1 Z2 Z3 S1 S2 S3 S4 S5 Z1 Z2 Z3 Z4 S1 S2 S3 S4 S5 Z1 Z2 Z3 those previous works linking oxidative stress and dormancy release (Nir et al, 1986;Scalabrelli et al, 1991;Or et al, 2002). On the other side, group DR was rich in stress and defence genes, as dormant tissues must be prepared against drought and cold stresses appearing during winter time.…”

Section: Discussionsupporting

confidence: 50%

“…Previously, other peroxidases have been related to the fulfillment of the chilling requirement in Vitis riparia buds (Mathiason et al, 2009) and to dormancy release in buds of leafy spurge (Jia et al, 2006). Earlier studies suggest a role of hydrogen peroxide (H 2 O 2 ) in budbreak regulation, based on catalase activity inhibition and H 2 O 2 accumulation occurring during the natural or hydrogen cyanamide induced breaking of bud dormancy in grapevine (Nir et al, 1986;Pérez et al, 2008). This points to a putative detoxifying role of peroxidases by degrading H 2 O 2 produced during budbreak.…”

Section: Discussionmentioning

confidence: 99%

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Molecular aspects of dormancy in peach (Prunus persica [L.] Batsch.)

Leida¹

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En estos cuatros años en los que he estado trabajando, he conocido a mucha gente que me ha ayudado en distintas formas para la realización de esta tesis y por eso he pensado en agradecerlos.En primer lugar quería dar las gracias a mis directores por la dedicación que me han prestado. A Gerardo y Marisa por darme la oportunidad de trabajar en el IVIA y de desarrollar esta tesis. A Gabino por haber estado ahí en todo momento, para enseñar y contestar a mis preguntas, sin su apoyo no habría podido llegar hasta aquí. ABSTRACTDormancy is one of the most important adaptive mechanisms developed by perennial plants, in order to survive the low temperatures of autumn and winter in temperate climates. The study of the genes regulated during dormancy release is crucial to understand the process, with the final objective of the development of new varieties with a better adaptation to certain environments; and this is particularly important considering the increasing economical weight of fruit crops in low and medium chilling regions as the Mediterranean area. We focused on the molecular and physiological mechanisms underlying the maintenance and release of seasonal dormancy in peach. In order to achieve this we first used suppression subtractive hybridization (SSH) to identify genes expressed in dormant and dormancy-released buds in two cultivars with different chilling requirements, 'Zincal-5' and 'Springlady', and subsequently validated their differential expression utilizing a peach cDNA microarray platform containing transcripts enriched in flower buds. Additionally, we carried out a genome-wide search of peach genes related to dormancy release by hybridizing the previous cDNA microarray with mRNA samples from 10 cultivars showing different dormancy behaviour, followed by an expression correlation analysis.Among the most relevant genes identified in these two first works, we found the DORMANCY ASSOCIATED MADS-box genes DAM4, DAM5 and DAM6, described independently by other groups working in peach and other species. The central role of DAM genes in dormancy regulation has also been confirmed by additional functional approaches as the analysis of the non-dormant evg mutant, QTL analysis, and transgenic approaches.In our second work we focused on the molecular mechanisms of DAM6 down-regulation concomitant with dormancy release in flower buds. A ChIP analysis of DAM6 promoter and structural gene revealed chromatin modification events similar to those observed in vernalization of Arabidopsis and cereals. We showed that DAM6 is transcriptionally active in dormant buds collected in October, when a short chromatin region around its ATG was trimethylated in histone H3 at K4 (H3K4) and acetylated at the N-terminal tail of H3. Concomitantly with DAM6 repression, H3K4 became demethylated and H3 deacetylated. Later H3K27 was found trimethylated along a genomic region larger than 4kb, including promoter, coding sequence and intron. Due to their relevance in dormancy regulation, DAM genes could be used as expression markers to assess...

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Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Molecular aspects of dormancy in peach (Prunus persica [L.] Batsch.)

Leida¹

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“…Activity of CAT is altered due to metabolic changes during various growth stages. In addition many environmental factors such as light (Feierabend et al, 1992) temperature (Omran, 1980;Nir et al, 1986), O 2 and CO 2 concentration (Fair et al, 1973;Monk et al, 1987) have been shown to influence catalase activity in mature plants. In fact this high level of CAT activity induced by grains drying was associated with a decrease in hydrogen peroxide level and in lipid peroxidation studied by Bailly et al (2004).…”

Section: Discussionmentioning

confidence: 99%

Comparative Antioxidant Potential and Bioactivity of Maize (Zea mays) Ear Tissues from Different Genotypes

Shahzadi¹,

Sheikh²,

Hameed³

et al. 2015

IJAB

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The aim of this study was to determine the effects of maize (Zea mays L.) in diabetic patients in vitro. Three varieties (Golden, Sultan and Agatti: 85) were used to study the level of antioxidants and bio-molecules. Results showed that maize ears parts of all three varieties were more effective inhibitor in the oxidation process. Variety Golden silks was found to contain abundant activity of protease (22488 units/g f.wt.), α-amylase (390 units /g f.wt.), total sugar contents (307 mg/g f.wt.), and nonreducing sugar (288 mg/g f.wt.), and husks were rich in reducing sugar contents (16.9 mg/g f.wt.), and grains (kernels) in (15 mg/g f.wt.) protein content. Variety Sultan silks contained total phenolic content (38100 µM/g f.wt.), husk contained peroxidase activity (88655 U/g f.wt.), and grains manifested catalase (555 U/g f.wt.) activity. In varietyAgatti85 a highest activity of superoxide dismutase (292 U/g f.wt.) and malondialdehyde content (35 µM/g f.wt.) was recorded in silks. The findings of this research showed that maize probably inhibits the reaction of oxidation and decreases the complications occurring in diabetes mellitus. It was concluded that performed active parameters (total phenolics, SOD, peroxidase, catalase) may be responsible for higher antioxidant activity, which can be successfully used to reduce the extent of diabetes in the patients.

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“…In such areas, the chilling requirement for budbreak are not fulfilled, leading to a late leafing, reduced and no regular sprouting and, consequently, decrease in yield, causing significant economic loses. Chilling exposing necessary for normal bud growth ranges between 50 and 400h at temperatures of 7.0 o C, varying for each grapevine cultivar and 'Niagara Rosada' grapevines needs about 100 h (DOKOOZLIAN et al, 1998), Previous literature indicates that the exposition of the buds to chilling inhibits the activity of the catalase, enzyme present in aerobics cells that decomposes the hydrogen peroxide (H2O2) in molecular oxygen and H2O (NIR et al, 1986). Its physiologic function eliminates the excess of H2O2 produced during the cellular metabolism, avoiding its accumulation and consequent cellular damage.…”

Section: Introductionmentioning

confidence: 99%

“…After that, the plant leads to H2O2 deintoxication through a sequence of reactions connected to the pentose phosphate pathway, leading to an increase of reduced nucleotides NAD(P)H, raising the metabolism and the induction of dormancy termination, bud burst and rapid growth (NIR et al, 1986). According to PINTO et al (2007), these metabolic changes has as consequence the increase on the levels of AMP/ATP intracellular relation that induces proteinkinases SNF, that plays a role in the transduction signal system for endodormancy end of buds.…”

Section: Introductionmentioning

confidence: 99%