Catalase activity in grapevine (Vitis vinifera L.) buds cv. ;Perlette.' increased to a maximum in October and thereafter decreased within 3 months to less than half its maximal rate. The decrease in catalase activity coincided with the decline in temperature during winter. The rate of sprouting of buds forced at 23 degrees C was negatively related to the activity of catalase. Artificial chilling of grapevine canes at 5 degrees C resulted in a 25% decrease of catalase activity in the buds after 3 days and 31% after 17 days. The activity of catalase increased to the control level only 96 hours after removing canes from 5 degrees C to room temperature. Efficient buddormancy breaking agents, such as thiourea and cyanamide decreased catalase activity to 64 and 50% of the controls respectively, while the activity of peroxidase remained the same under those conditions. A less efficient dormancy breaking agent dinitro-ortho-cresol, did not decrease catalase activity.
[14C]ethephon was applied to different organs of detached and intact shoots of some grapevine cultivars. Recovery of 14C-labelled compounds, 48 h after application indoors to detached leaves, was 53-61% of the amount applied. Recovery was between 58 and 72% of the amount applied 4 h earlier to different sites on young intact shoots of potted vines, in a greenhouse. Uptake of labelled compound was only 19-26% of the amount applied. Translocation of [14C]ethephon was very slight. Most of the applied labelled compound remained at the application sites for many hours. Translocation was mainly basipetal as confirmed also by autoradiography.
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