Changes in Motility Characteristics of Goat Spermatozoa During Glycerol-Equilibration and the Relevance to Cryopreservation

Sundararaman, M.N.; Edwin, M. J.

doi:10.3923/ajcb.2008.22.33

Cited by 18 publications

(12 citation statements)

References 34 publications

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“…In the study by Leite et al (2010), zero equilibration time resulted in significantly low post-thawed sperm motility, compared to equilibration times of either 2 or 4 hours. It has been reported that the best equilibration time in buck semen was 2 hours (Sundaraman and Edwin 2008), between 2 to 4 hours in boar (Schäfer et al, 2017), 4 hours for bulls (Leite et al, 2010) and 6 hours for dog (Belala et al, 2016b).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times

Amal

Arifiantini

Setiadi

et al. 2019

J. Indonesian Trop. Anim. Agric.

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ABSTRAKTujuan dari penelitian ini adalah untuk menguji efektivitas dari tiga macam bahan pengencer (Tris kuning telur, Bioxcell® dan Optixcell®) dan tiga waktu ekuilibrasi (T-30 min, T-2 jam dan T-4 jam) untuk kriopreservasi semen sapi bali. Tiga puluh enam ejakulat semen berasal dari 6 ekor sapi bali jantan dikoleksi dan dibekukan mengikuti protokol pembekuan rutin. Evaluasi motilitas sperma postthawing (PTM) dilakukan dengan computer assisted semen analysis (CASA). Integritas membran plasma dievaluasi dengan hypo-osmotic swelling (HOS) test dan viabilitas dievaluasi dengan pewarnaan eosin nigrosin. Hasil penelitian menunjukkan terdapat interaksi yang signifikan antara waktu ekuilibrasi dan pengencer terhadap motilitas, viabilitas dan integritas membran sperma sapi bali. Perlakuan kontrol (4 jam waktu ekuilibrasi) memiliki nilai tertinggi (P<0,05) dan perlakuan 30 menit waktu ekuilibrasi memiliki nilai terrendah (P<0,05) untuk total motilitas, motilitas progresif, persentase sperma dengan membran plasma utuh (MPU) dan viabilitas sperma. Motilitas, skor pergerakan individu dan integritas membran sperma dalam pengencer Optixcell® lebih tinggi dibandingkan dengan semen yang dibekukan dalam pengencer Bioxcell® dan Tris kuning telur. Motilitas total, progresif dan integritas membran sperma terbaik secara keseluruhan terdapat dalam pengencer Tris kuning telur dan Bioxcell® dengan waktu ekuilibrasi 4 jam dan Optixcell® dengan waktu ekuilibrasi 2 dan 4 jam. Kesimpulan berdasarkan analisis subyektif dan obyektif, equilibrasi 30 menit tidak cukup untuk mempertahankan motilitas dan integritas membran sperma yang lebih baik; equilibrasi untuk 2 jam menghasilkan motilitas sperma tertinggi untuk pengencer Optixcell®; dan equilibrasi untuk 4 jam menghasilkan viabilitas tertinggi untuk semua pengencer yang digunakan.Kata kunci: sapi bali, kriopreservasi semen, pengencer semen, waktu ekuilibrasi, CASA. ABSTRACTThe objectives of the present study were to compare and determine the best post-thawed characteristics of balinese bull sperm cryopreserved in three different extenders; animal based (Trisclarified egg yolk (Tris-cEY)), and non-animal based extenders (Bioxcell® (lecithin based) andCharacteristics of Post-thawed Bali Bull Semen (A.S. Amal et al.) 135 Optixcell® (liposome based)) in combination with three different equilibration times (30 minutes, 2 hours, 4hours). Thirty six ejaculates were collected from six Balinese bulls and frozen in three extenders (Tris-cEY, Bioxcell® and Optixcell®) after equilibration in three different times (30 minutes, 2hours and 4hours). Computer-assisted sperm analysis (CASA), hypo-osmotic swelling test (HOST) and eosin nigrosin staining were used in the post-thawed semen analysis. There was a significant interaction between equilibration time and extender type for sperm motility, viability and membrane integrity. Thirty minutes equilibration time had the lowest values (P<0.05) for all the evaluated parameters independent of extender type. Overall, semen extended in Tris-cEY, Bioxcell® and O...

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Section: Discussionmentioning

confidence: 99%

Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times

Amal

Arifiantini

Setiadi

et al. 2019

J. Indonesian Trop. Anim. Agric.

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“…Furthermore, membrane destabilization might occur when the sperm plasma membrane undergoes a phase transition from the liquid crystalline phase to the gel phase due to a decrease in temperature during cryopreservation (Sundararaman and Edwin, 2008). The irreversible changes in the sperm membrane induced by lipid phase transitions during cooling warming may possibly affect the movement characteristics of spermatozoa during semen processing for cryopreservation (Deleeuw et al, 1990).…”

Section: Resultsmentioning

confidence: 99%

Post-Thaw Evaluation of Cryopreserved Boer Crossbred Buck Semen Extended in TEYG (Universal) Extender and its Fertility Rate

Manivannan¹,

Selvaraju²,

Napolean³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…During freezing, the sperm plasma membrane undergoes a phase transition from the liquid crystalline phase to the gel phase due to a decrease in temperature. This irreversible changes induced by lipid phase transitions during cooling warming may possibly affect the movement characteristics of spermatozoa during semen processing [13]. According to Muiño et al [9], ejaculates with the highest populations of rapid and progressive sperm were also the most resistant to cryopreservation and showed the best post-thaw sperm longevity.…”

Section: Discussionmentioning

confidence: 99%

Sperm subpopulations, buffalo semen, sperm kinematics, cryopreservation, computer assisted sperm analyzer.

Maylem¹,

Leoveras²,

Venturina³

et al. 2017

JAST-A

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Abstract:This study aimed to identify and characterize the sperm subpopulations existing in water buffalo semen using a computer assisted sperm analyzer (CASA), as well as assess the effects of cryopreservation on the sperm subpopulation structure and evaluate bull variability. The semen of eight Bulgarian Murrah bulls was collected by four times in an interval of one week each. The semen was cryopreserved following a standard protocol and sperm kinematics was assessed. Clustering methods were applied to individual sperms, forming two significantly different (P < 0.05) subpopulations (P 1 and P 2 ). Subpopulation P 1 represents those spermatozoa that moved most rapidly and progressively (46.29%), and subpopulation P 2 includes spermatozoa with relatively low velocity or poorly motile but with high progressiveness (53.41%). There was a decline on the population of P 1 sperms from fresh (52.52%), pre-freeze (45.73%) to post-thaw (35.17%) stages and significant difference on the sperm kinematics between P 1 and P 2 . A significant decline in the values of distance, velocity and amplitude of lateral head (ALH) parameters were observed at post-thaw stage, while an increase was observed on trajectory and beat cross frequency (BCF) kinematics. Values of sperm kinematics were also significantly different (P < 0.05) among all bulls. The frequency distribution of spermatozoa on both subpopulations P 1 and P 2 was quite similar for all bulls in pre-freeze and post-thaw stages, but with significant (P < 0.05) variability on fresh stage. Bulls with the highest maintained frequency of P 1 sperms are denoted as good freezer bulls. In sum, kinematic characterization of water buffalo sperm and clustering into subpopulation enable to identify bulls that are more resistant to cryopreservation and production of quality semen for genetic propagation.

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Changes in Motility Characteristics of Goat Spermatozoa During Glycerol-Equilibration and the Relevance to Cryopreservation

Cited by 18 publications

References 34 publications

Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times

Characteristics of the post-thawed Balinese bull semen extended in three different extenders and equilibration times

Post-Thaw Evaluation of Cryopreserved Boer Crossbred Buck Semen Extended in TEYG (Universal) Extender and its Fertility Rate

Sperm subpopulations, buffalo semen, sperm kinematics, cryopreservation, computer assisted sperm analyzer.

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