The nature of cervical mucus and the change in the pH of cervical mucus and uterine flushings in 72 crossbred cows were studied. Six groups (12 cows in each group) received treatment viz. 30 ml of 2 per cent Lugol's iodine (Group I), 30 ml PBS containing 100 µg of E.coli LPS (Group II), 2 mg of lysozyme (Group III), 500 mg of oyster glycogen (Group IV), 25 mg PGF 2 α (Group V) and 30 ml PBS intrauterine on the day of oestrus (Group VI, control). The mean pH value of the cervical mucus and uterine fluid before treatment was ranged from 8.47 ± 0.02 to 8.62 ± 0.02 and 7.87 ± 0.04 to 8.30 ± 0.02 and after the treatment it ranged from 7.01 ± 0.02 to 8.17 ± 0.04 and 7.61 ± 0.01 to 7.97 ± 0.01 in all the treated and control groups, respectively with a significant (P≤0.01) reduction in the pH value. The cows were inseminated with good quality frozen semen after giving sexual rest for one cycle. The highest conception rate was obtained in E. coli LPS group (83.33 per cent), followed by lysozyme group (66.67 percent) and oyster glycogen group (66.67 per cent), respectively.

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