Changes in composition and content of food‐derived peptide in human blood after daily ingestion of collagen hydrolysate for 4 weeks

Shigemura, Yasutaka; Suzuki, Asahi; Kurokawa, Mihoko; Satō, Yoshio; Satô, Kenji

doi:10.1002/jsfa.8677

Cited by 33 publications

(32 citation statements)

References 35 publications

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“…Identification of food-derived peptides in blood Hyp-containing peptides; Pro-Hyp, Pro-Hyp-Gly, Ile-Hyp, Leu-Hyp, Hyp-Gly, Glu-Hyp, Ala-Hyp, and Pro-Gly have been identified (Shigemura et al, 2017). In most cases, Pro-Hyp accounts for more than 50% of total collagen peptides in human plasma.…”

Section: Introductionmentioning

confidence: 99%

Identification of food-derived peptides in human blood after ingestion of corn and wheat gluten hydrolysates

Ejima¹,

Nakamura²,

Suzuki³

et al. 2018

JFB

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Bioactive peptides in the body after ingestion of plant protein hydrolysates have been speculated but not identified. We aimed to establish an approach to identify small amounts of food-derived peptides in humans after ingestion of non-extracellular matrix protein hydrolysates. Corn and wheat gluten hydrolysates were digested using pancreatin and leucine aminopeptidase; the resultant peptides were identified via size-exclusion chromatography and reverse-phase HPLC-tandem mass spectrometry (MS/MS). Structures of indigestible peptides were confirmed via LC-MS/MS in multi-reaction monitoring mode. All indigestible peptides in the exopeptidase digest were diprolyl and di- and tripyroglutamyl peptides. Blood collected from healthy volunteers (n = 4) before and after ingestion of 9 g of the hydrolysates was assessed for the indigestible peptides via LC-MS/MS. Six peptides (Pro-Ala, Pro-Gly, Pro-Gln, pyroGlu-Pro, pyroGlu-Leu-Pro, and pyroGlu-Gln-Pro) significantly increased in human plasma up to 10–100 nM compared to the baseline. This may hence be a powerful tool for identifying foodderived peptides in blood.

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Section: Introductionmentioning

confidence: 99%

Identification of food-derived peptides in human blood after ingestion of corn and wheat gluten hydrolysates

Ejima¹,

Nakamura²,

Suzuki³

et al. 2018

JFB

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“…Daily intake of collagen hydrolysate has been reported to improve skin and joint conditions, and these beneficial effects are exerted by hydroxyproline (Hyp) containing peptides, which were detected in human blood soon after ingestion ( Shigemura et al., 2017 ). Daily intake of collagen hydrolysate for an extended period can change compositional rate of Hyp-containing peptides in human blood.…”

Section: Resultsmentioning

confidence: 99%

Bioaccessibility of Shore Magic® collagen, a low-molecular-weight collagen supplement, in different in vitro barrier models

Benadiba

Serruya

Maor

2018

Heliyon

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Hydrolyzed collagen consists of peptides, which exert important biological functions in different body systems. This study aimed at testing the biological effects of a low molecular weight collagen (LMWC), namely Shore Magic® Collagen (SMC), in a series of in vitro assays and three different in vitro barrier models with translational significance. We also compared SMC's biological activities with its trypsinized form (TSMC). SMC enhanced migration in both epithelial and endothelial cells; and increased the adhesion of epithelial cells, but surprisingly not of endothelial cells. It also diminished the tightness in the gut and blood-brain barriers in vitro while TSMC did not. SMC induced both neurogenesis and BJ epithelial cell proliferation of cells growing below the in vitro barriers. In conclusion, the intact form of SMC shows enhanced bioavailability and efficiency compared with TSMC.

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“…The human studies were carried out according to a protocol described previously [ 15 , 29 , 30 ]. These studies were performed according to the Helsinki Declaration under the supervision of medical doctors and were approved by the experimental ethical committees of the Qsai Corporation (Fukuoka, Japan).…”

Section: Methodsmentioning

confidence: 99%

A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate

et al. 2018

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Levels of short linear hydroxyproline (Hyp)-containing peptides, such as prolyl-hydroxyproline (Pro-Hyp), increase in human blood after the ingestion of collagen hydrolysate, which has been associated with beneficial effects for human skin and joints. The present study demonstrates the presence of a novel food-derived collagen peptide, cyclic Pro-Hyp, in human blood after the ingestion of collagen hydrolysate. The cyclic Pro-Hyp levels in plasma samples were estimated by liquid chromatography mass spectrometry (LC-MS). Cyclic Pro-Hyp levels significantly increased in the plasma after ingestion of collagen hydrolysate, reaching a maximum level after 2 h and then decreasing. The maximum level of cyclic Pro-Hyp in plasma ranged from 0.1413 to 0.3443 nmol/mL, representing approximately 5% of linear Pro-Hyp in plasma after ingestion of collagen hydrolysate. Addition of cyclic Pro-Hyp in medium at 7 nmol/mL significantly enhanced the growth rate of mouse skin fibroblasts on collagen gel more extensively compared to linear Pro-Hyp.

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Changes in composition and content of food‐derived peptide in human blood after daily ingestion of collagen hydrolysate for 4 weeks

Cited by 33 publications

References 35 publications

Identification of food-derived peptides in human blood after ingestion of corn and wheat gluten hydrolysates

Identification of food-derived peptides in human blood after ingestion of corn and wheat gluten hydrolysates

Bioaccessibility of Shore Magic® collagen, a low-molecular-weight collagen supplement, in different in vitro barrier models

A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate

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