Identification of food-derived peptides in human blood after ingestion of corn and wheat gluten hydrolysates

Ejima, Akika; Nakamura, Megumi; Suzuki, Yasushi; Satô, Kenji

doi:10.31665/jfb.2018.2145

Cited by 30 publications

(48 citation statements)

References 30 publications

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“…In addition, various peptides can be transported across the intestinal epithelial membrane. This is the case was derived-milk peptides or corn and wheat gluten hydrolysates, which have been identified in the human bloodstain after ingestion [57,58]. Common peptide transport routes are at circulating bloodstain carrier-mediated permeation, transcytosis, paracellular transport or passive diffusion.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Anti-Inflammatory and Atheroprotective Properties of Wheat Gluten Protein Hydrolysates in Primary Human Monocytes

Paz

Rodríguez-Martín

Villanueva

et al. 2020

Foods

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Bioactive protein hydrolysates have been identified in several sources as possible agents in the prevention and treatment of many diseases. A wheat gluten (WG) concentrate was hydrolyzed by Alcalase under specific conditions. The resulting hydrolysates were evaluated by in vitro cell-free experiments leading to the identification of one bioactive WG protein hydrolysate (WGPH), which was used at 50 and 100 μg/mL on primary human monocytes. Reactive oxygen species (ROS) and nitrite levels and RT-qPCR and ELISA techniques were used to analyze the functional activity of WGPH. Our results showed that WGPH hydrolyzed in 45 min (WGPH45A) down-regulated gene expression of Interleukin (IL)-1β, IL-6, IL-17, and Interferon gamma (IFNγ) and reduced cytokine release in lipopolysaccharide (LPS)-stimulated monocytes. In addition, WGPH45A down-regulated gene-related to atherosclerotic onset. Our results suggest that WGPH45A has a potent anti-inflammatory and atheroprotective properties, reducing the expression of gene-related inflammation and atherosclerosis that could be instrumental in maintaining cardiovascular homeostasis.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Anti-Inflammatory and Atheroprotective Properties of Wheat Gluten Protein Hydrolysates in Primary Human Monocytes

Paz

Rodríguez-Martín

Villanueva

et al. 2020

Foods

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“…To the best of our knowledge, there is no study demonstrating the enhancement of the production of host antimicrobial peptides by the oral administration of a single food component. It has been shown that pyroGlu-Leu is widely distributed in food protein hydrolysates, such as wheat gluten and corn gluten hydrolysates, 7 as well as in Japanese fermented foods produced by Asperigillus oryzae , such as the Japanese rice wine known as sake, the salted fermented soy paste known as miso, and a type of soy sauce, shoyu. 15 Fermented foods are consumed in the diet, while protein hydrolysates are generally consumed as supplement.…”

Section: Discussionmentioning

confidence: 99%

“…3,4 Food protein hydrolysates and fermented foods contain pyroglutamyl peptides, which are spontaneously generated from peptides with a glutaminyl residue at the amino terminal during storage and processing. 5–7 Short chain pyroglutamyl peptides are resistant to digestion by endoproteinases and exopeptidases. Some of the short chain pyroglutamyl peptides have been demonstrated to have in vivo 8–11 and in vitro 12,13 activities.…”

Section: Introductionmentioning

confidence: 99%

Pyroglutamyl leucine, a peptide in fermented foods, attenuates dysbiosis by increasing host antimicrobial peptide

et al. 2019

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PyroGlu-Leu is present in certain food protein hydrolysates and traditional Japanese fermented foods. Our previous study demonstrated that the oral administration of pyroGlu-Leu (0.1 mg/kg body weight) attenuates dysbiosis in mice with experimental colitis. The objective of this study was to elucidate why such a low dose of pyroGlu-Leu attenuates dysbiosis in different animal models. High fat diet extensively increased the ratio of Firmicutes/Bacteroidetes in feces of rats compared to control diet. Oral administration of pyroGlu-Leu (1 mg/kg body weight) significantly attenuated high fat diet-induced dysbiosis. By focusing on the production of intestinal antimicrobial peptides, we found that pyroGlu-Leu significantly increased the level of 4962 Da peptides, which identified as the propeptide of rattusin or defensin alpha 9, in ileum. We also observed increased tryptic fragment peptides from rattusin in the lumen. Here, we report that orally administered pyroGlu-Leu attenuates dysbiosis by increasing in the host antimicrobial peptide, rattusin.

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“…To improve resolution and detection of compounds with primary and secondary amines, derivatization with AccQ, followed by LC-MS/MS, was performed (Ejima et al, 2018). Aliquots (200 μL) of SEC fractions were dried under vacuum.…”

Section: Liquid Chromatography-tandem Mass Spectrometry (Lc-ms/ms) Anmentioning

confidence: 99%

Identification of a compound, at a very low dose (less than 100 ng/g of diet), with lifespan-elongation activity towards SOD-1 mutant adults of Drosophila melanogaster in the hot water extract of Chlorella pyrenoidosa

Zheng¹,

Inoué²,

Kohno³

et al. 2020

JFB

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Hot water extract of chlorella (WEC) increased the lifespan of superoxide dismutase (SOD)-1 mutant adults of Drosophila melanogaster in a dose dependent manner (200–800 µg/mL). Compounds in WEC were successively fractionated by solid phase extraction using a Sep-Pak C18 cartridge and size exclusion chromatography (SEC). Amino compounds in SEC fractions were derivatized with 6-aminoquinolyl-N-hydroxylsuccinimidyl carbamate and analyzed by reversed phased-liquid chromatography-tandem mass spectrometry. Phenylalanine, phenethylamine, isopentylamine, and 2-methylbutylamine were identified in the SEC fraction, which increased the lifespan of the D. melanogaster mutant adults. Phenethylamine, at very low doses (6–60 ng/g of diet) that roughly corresponded to those of phenethylamine in WEC (200–800 μg/mL), increased the lifespan of the D. melanogaster adults, while isopentylamine did not exert the lifespan elongation activity. Since phenethylamine did not show SOD-like activity, it did not increase lifespan by direct antioxidant activity.

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Identification of food-derived peptides in human blood after ingestion of corn and wheat gluten hydrolysates

Cited by 30 publications

References 30 publications

Evaluation of Anti-Inflammatory and Atheroprotective Properties of Wheat Gluten Protein Hydrolysates in Primary Human Monocytes

Evaluation of Anti-Inflammatory and Atheroprotective Properties of Wheat Gluten Protein Hydrolysates in Primary Human Monocytes

Pyroglutamyl leucine, a peptide in fermented foods, attenuates dysbiosis by increasing host antimicrobial peptide

Identification of a compound, at a very low dose (less than 100 ng/g of diet), with lifespan-elongation activity towards SOD-1 mutant adults of Drosophila melanogaster in the hot water extract of Chlorella pyrenoidosa

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