A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate

Shigemura, Yasutaka; Iwasaki, Yu; Tateno, Mana; Suzuki, Asahi; Kurokawa, Mihoko; Satō, Yoshio; Satô, Kenji

doi:10.3390/nu10101356

Cited by 19 publications

(15 citation statements)

References 36 publications

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“…[20] Of note, in our sex-specific analysis, another sphingolipid SM (d18:1/24:1) was suggestively associated with BMD status in our female participants only (P=0.03 and 0.04 in discovery and replication set, respectively). Circulating isoleucyl-proline may exert antioxidant effects, [45] and it may explain its association with BMD in our study to some extent. Lactic acid, which was associated with BMD in our discovery set only, has been previously reported to be associated with a decreased risk of low BMD in Taiwanese women.…”

Section: Discussionmentioning

confidence: 55%

Association between the metabolome and bone mineral density in a Chinese population

et al. 2020

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Section: Discussionmentioning

confidence: 55%

Association between the metabolome and bone mineral density in a Chinese population

et al. 2020

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“…Moreover, the amino acid composition of the peptides is reflected by a high content of specific amino acids that are abundant building blocks of human collagen, such as hydroxyproline, proline, glycine, glutamic acid, alanine, and arginine [7,31,32]. During digestion, the oligopeptides are further metabolized to bioactive di- and tri-peptides in the gastrointestinal tract and are subsequently released into the blood stream [11,33,34,35,36,37,38]. The concentration and type of collagen, as well as the content of other skin-relevant nutrients can differ in mono and combination products.…”

Section: Discussionmentioning

confidence: 99%

A Collagen Supplement Improves Skin Hydration, Elasticity, Roughness, and Density: Results of a Randomized, Placebo-Controlled, Blind Study

Bolke¹,

Schlippe²,

Gerß³

et al. 2019

Nutrients

122

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The purpose of this randomized, placebo-controlled, blind study was to investigate the effects of the drinkable nutraceutical ELASTEN ® (QUIRIS Healthcare, Gütersloh, Germany) on skin aging and skin health. Drinking ampoules provides a blend of 2.5 g of collagen peptides, acerola fruit extract, vitamin C, zinc, biotin, and a native vitamin E complex. This controlled interventional trial was performed on 72 healthy women aged 35 years or older. They received either the food supplement (n = 36) or a placebo (n = 36) for twelve weeks. A skin assessment was carried out and based on objective validated methods, including corneometry (skin hydration), cutometry (elasticity), the use of silicon skin replicas with optical 3D phase-shift rapid in-vivo measurements (PRIMOS) (roughness), and skin sonography (density). The verum group was followed for an additional four weeks (without intake of the test product) to evaluate the sustainability of the changes induced by the intake of the test product. The test product significantly improved skin hydration, elasticity, roughness, and density. The differences between the verum group and the placebo group were statistically significant for all test parameters. These positive effects were substantially retained during the follow-up. The measured effects were fully consistent with the subjective assessments of the study participants. The nutraceutical was well tolerated.

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“…14 Most of these Pro-Hyp-derived metabolites are unidenti ed, but some are reported to be converted into cyclic Pro-Hyp. 29 Our ndings indicate that ACOP is involved in such Pro-Hyp metabolism. Therefore, dietary ACOP supplementation may be useful for rapid compensation for Pro-Hyp consumption during physical exertion.…”

Section: Discussionmentioning

confidence: 64%

Monitoring Urinary Collagen Metabolite Changes Following Collagen Peptide Ingestion and Physical Activity Using ELISA With Anti–Active-Collagen-Oligopeptide Antibody

Osawa

Nomura

Kimira

et al. 2021

Preprint

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Active collagen oligopeptides are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC-MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism.

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A Pilot Study for the Detection of Cyclic Prolyl-Hydroxyproline (Pro-Hyp) in Human Blood after Ingestion of Collagen Hydrolysate

Cited by 19 publications

References 36 publications

Association between the metabolome and bone mineral density in a Chinese population

Association between the metabolome and bone mineral density in a Chinese population

A Collagen Supplement Improves Skin Hydration, Elasticity, Roughness, and Density: Results of a Randomized, Placebo-Controlled, Blind Study

Monitoring Urinary Collagen Metabolite Changes Following Collagen Peptide Ingestion and Physical Activity Using ELISA With Anti–Active-Collagen-Oligopeptide Antibody

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