Bioaccessibility of Shore Magic® collagen, a low-molecular-weight collagen supplement, in different in vitro barrier models

Benadiba, Marcel; Serruya, Raphael; Maor, Yehoshua

doi:10.1016/j.heliyon.2018.e00821

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…Lower molecular weight molecules could penetrate into cells better than a higher molecular weight molecule, therefore leading to better cell or tissue functional improvement [59]. Furthermore, many studies have found that collagen with molecular weights of less than 5 kDa can increase cell proliferation, migration, adhesion, anti-oxidization, or angiotensin-I converting enzyme (ACE) inhibition activity [36,60,61,62]. Therefore, the molecular weight of collagen could be considered as a significant factor for collagen selection.…”

Section: Resultsmentioning

confidence: 99%

Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes

Thaweekitphathanaphakdee

Chanvorachote

Prateepchinda

et al. 2019

Marine Drugs

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Stem cell activities in human tissues are critical for tissue integrity and function. Maintaining keratinocyte stem cells (KSCs) stemness helps sustain healthy skin by supporting keratinocyte renewal, involving the formation of epidermal barriers. In this study, abalone collagen (AC) extracts with molecular weights of 3 kDa (AC 1) and 300 kDa (AC 2) were compared to the epidermal growth factor (EGF) for their effects on cell proliferation, cell migration (wound healing), spheroid formation, and the expression level of stem cell markers on human keratinocytes (HaCaT cells). Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell proliferation was quantified by ATP and DNA content analysis and Sulforhodamine B (SRB) assays. Cell migration assay was determined using the scratch wound healing test. Spheroid formation was evaluated and the expression level of stem cell markers was investigated by western blot analysis. The results showed that AC 1 at the concentration of 100 µg/mL could stimulate HaCaT cell proliferation, migration, spheroid formation, and the expression level of stem cell markers (keratin 19, β-catenin, ALDH1A1) compared to the control. In conclusion, a smaller molecular weight of abalone collagen extract exhibits a better effect on keratinocytes proliferation, migration, and stemness, which could be a potential active ingredient in cosmeceutical products.

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Section: Resultsmentioning

confidence: 99%

Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes

Thaweekitphathanaphakdee

Chanvorachote

Prateepchinda

et al. 2019

Marine Drugs

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“…On the 7 th day, the angiogenesis phase reaches its peak. [ 2 3 ] It is also known that healing process is influenced by systemic factors, one of which is comorbidities, such as diabetes mellitus (DM). DM is known to cause macrophage dysfunction in the patients.…”

Section: Introductionmentioning

confidence: 99%

The efficacy of okra fruit extract on the expression of transforming growth factor beta 1 in the tooth socket of diabetic Wistar rats

Luthfi

Yuliati

Wijayanti

et al. 2021

Dental Research Journal

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Background: Patients with diabetes mellitus suffer from an additional macrophage dysfunction in the secretion of growth factor, which later decreases transforming growth factor beta 1 (TGF-β1). This condition disrupts proliferation and angiogenesis. Extract of okra fruit ( Abelmoschus esculentus ) contains flavonoid, an active substance which acts as antioxidant, anti-inflammation, and antidiabetes. The purpose of this study is to analyze the difference in TGF-β1 expression in wound-healing process after tooth extraction of diabetic Wistar rats. Materials and Methods: This is a laboratory experimental study using pretest and posttest on 24 Wistar rats which are divided into two groups: control group (treated with streptozotocin induction but without administration of okra fruit extract) and treatment group (treated with streptozotocin induction and oral administration of 250 mg/kg okra fruit extract once a day). Extractions of the rats’ mandibular left incisors were performed using a pair of modified forceps and an elevator. The tooth sockets were then irrigated using saline solution. Four rats in each group were sacrificed on day 3 (KO1, PO1), 5 (KO2, PO2), and 7 (KO3, PO3). The socket tissues from the rats were then immunohistochemically analyzed. Data were analyzed at level significance of 0.05. Results: The average level of TGF-β1 expression in the treatment groups was higher compared to the control group: PO1 (11.59 ± 0.58), PO2 (15.15 ± 1.07), and PO3 (18.75 ± 2.73) as compared to KO1 (5.32 ± 1.69), KO2 (8.47 ± 0.60), and KO3 (9.28 ± 1.16) with P = 0.001. Conclusion: The administration of okra fruit extract can increase the level of TGF-β1 in wounds after tooth extraction of diabetic Wistar rats.

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Bioaccessibility of Shore Magic® collagen, a low-molecular-weight collagen supplement, in different in vitro barrier models

Cited by 2 publications

References 18 publications

Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes

Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes

The efficacy of okra fruit extract on the expression of transforming growth factor beta 1 in the tooth socket of diabetic Wistar rats

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