Challenge of multi drug resistant stx1 harboring E. Coli in meat and fast foods

Amani, Samad; Shaimaa, N.; Mohamed, Khaled O.

doi:10.21608/bvmj.2017.30011

Cited by 3 publications

(1 citation statement)

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“…Based on this study, there was a high percentage of positive E. coli in Döner kebab meat samples (20.9%). The results were similar to the study conducted by Amani et al (2017) that showed 33. kebab that contaminated by E. coli from this studywas alarming because E. coli pathogen could cause foodborne disease to the consumers. Since the Döner kebab was mostly consumed by the students, it might cause the foodborne disease in the students.…”

Section: Discussionsupporting

confidence: 90%

Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

Sari¹,

Pisestyani²,

Lukman³

2020

Adv. Anim. Vet. Sci.

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This study was aimed to determine the occurrence of antibiotic resistance in Escherichia coli (E. coli) isolated from Döner kebab sold in Dramaga Bogor, West Java Province, Indonesia. A total of 43 samples of Dὂner kebab meat were taken from all of the vendors in the radius of 2 km around the border of IPB University campus, Dramaga Bogor. Isolation and identification of E. coli were referred to the Guideline for Laboratory Analysis on Examination of Microbial Contamination in Meat, Egg, and Milk in SNI 2897:2008 issued by the National Standardization Agency of Indonesia. The resistance against nine antibiotics were tested using Kirby-Bauer disk diffusion method based on the standard of Clinical Laboratory Standards Institute (CLSI) in 2018. The result showed that nine samples were positive containing E. coli (20.9%) and 84.2% of E. coli isolates were resistant to eight antibiotics: gentamicin (57.9%), ampicillin (26.3%), cefotaxime, trimethoprim-sulfamethoxazole, and ciprofloxacin (21.1%), amoxicillin-clavulanate (10.5%), enrofloxacin, and oxytetracycline (5.3%). All of E. coli isolates were susceptible to chloramphenicol (100%). There were 21.1% of isolates also resistant to three or more classes of antibiotics that were known as multi-drug resistant (MDR) with different resistant patterns.The presence of E. coli resistant antibiotic in kebab meat could results serious problems in human health.

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Section: Discussionsupporting

confidence: 90%

Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

Sari¹,

Pisestyani²,

Lukman³

2020

Adv. Anim. Vet. Sci.

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Occurrence and Antibiotic Resistance of Escherichia coli in Street-Vended Kebab in Dramaga, Bogor, Indonesia

Lukman¹,

Sari²,

Pisestyani³

2020

JFQHC

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Background: Kebab is one of the popular ready-to-eat foods in Indonesia and sold as a street food. The purpose of this study was to determine occurrence and antibiotic resistance of Escherichia coli in street-vended kebab in Dramaga, Bogor, Indonesia. Methods: Totally, 43 samples of kebab meat were collected from street food kebab vendors. Examinations on Total Plate Count (TPC), and total E. coli using Most Probable Number (MPN) method were referred to the Indonesia National Standard. The antibiotic resistance test was carried out using the Kirby-Bauer disk diffusion method. Results: The mean of TPC in kebab samples was 5.3 log10 Colony Forming Unit (CFU)/g. Based on TPC, 13 out of 43 (30.2%) of kebab samples did not comply with the Indonesia National Standard with maximum acceptable level of 5 log10 CFU/g. E. coli was identified in 5 out of 43 samples (11.6%) with mean of 39.2 MPN/g ranged from 7.4 to 150 MPN/g which were higher than standard level (0.5 log10 CFU/g). Four out of 5 E. coli isolated from kebab samples were resistant to gentamycin. All E. coli isolates were susceptible to amoxicillin/clavulanate and chloramphenicol. Conclusion: The occurrence of antibiotic resistant E. coli in ready-to-eat kebab in this area of Indonesia could cause the health problem in consumers. The local government should conduct the monitoring and surveillance on the occurrence of pathogens and the antibiotic resistance in food chain.

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Molecular detection of virulence factors in some food poisoning bacteria isolated from chicken meat and giblet

ElDin¹,

Saad

Ibrahim

et al. 2020

Benha Veterinary Medical Journal

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Many bacterial detection rapid methods developed including nucleic acid-based analysis which considered the most precise, sensitive, and famous method of detection. This study aimed to investigate the bacterial hygienic quality of some chicken meat and giblet with special concern of molecular detection of some virulence factors associated with some isolated food poisoning bacteria. E. coli, Salmonella, S. aureus, and Y. enterocolitica strains were isolated from commercial and home-reared chicken meat and giblet in Menoufiya Governorate, Egypt. Accurately, stx1, stx2, eaeA, and hylA genes were detected in 45.4, 63.6, 18.1, and 27.2% of the isolated E. coli strains, respectively. invA, hilA, and fimH genes were detected in 100, 71.4, and 85.7% of the examined Salmonella isolates, respectively. Regarding to the examined Y. enterocolitica isolates, Inv gene was detected lonely in 25%, while it was mixed with ystA gene in 75% of the examined isolates. Detection of enterotoxigenic Staph. aureus genes revealed detection of staphylococcal enterotoxins genes types SEA, and SEB genes in 20, and 10%; moreover, mixed SEA+SED, SEB+SEC producing genes were detected in 10% for each, respectively. The present results proved that PCR assay is helpful, rapid and accurate detection method. Strict hygienic measures during slaughtering and handling of chicken meat and giblet must be followed.

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Challenge of multi drug resistant stx1 harboring E. Coli in meat and fast foods

Cited by 3 publications

References 0 publications

Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

Occurrence and Antibiotic Resistance of Escherichia coli in Street-Vended Kebab in Dramaga, Bogor, Indonesia

Molecular detection of virulence factors in some food poisoning bacteria isolated from chicken meat and giblet

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