Cell-Mediated Immune Response of in Vitro Sensitized Lymphocytes to Isogeneic Methyl-Cholanthrene-Induced Tumor Cell Lines

In vitro induction of tumour-specific immunity V. Detection of common antigenic determinatnts of murine fibrosarcomas

“…The in vivo Winn assay results showed clear-cut specificity of in vitro immunization to "unique" TAA on each of the tumours, but the in vitro 3H-thymidine-release assay with the same CL demonstrated some cross-reactivity between the 2 fibrosarcomas. Subsequent studies have confirmed the in vitro immunization of lymphocytes by TAA on carcinogen-induced tumours (Warnatz and Scheiffarth, 1974;Small and Trainin, 1975;Kall and Hellstrom, 1975). However, conflicting results have emerged from these in vitro experiments, with claims both that the specificity of the in vitro tumour specific immunity induced is to unique TAA alone (McKhann and Jagarlamoody, 1971;Kall, Hellstrom and Hellstrom, 1976) and that it is to both crossreacting and unique TAA on the same tumour cells (Warnatz and Scheiffarth, 1974).…”

In vitro induction of tumor-specific immunity. IV. Specific adoptive immunotherapy with cytotoxic T cells induced in vitro to plasmacytoma antigens

“…More recently interest in this type of immunotherapy has centred on attempts to immunise lymphocytes in vitro to tumor antigens. This has resulted from the desire to avoid inoculating patients with malignant cells, albeit killed, and the demonstration in animal models that cytotoxic thymus-derived lymphocytes (T cells) can be induced in vitro to tumor-associated antigens (TAA) (McKhann and Jagarlamoody, 1971 a;Wagner and Rollinghoff, 1973;Lundak and Raidt, 1973;Warnatz and Scheiffarth, 1974;Nomoto et al, 1974;Burton et al, 1975;Kuperman et al, 1975;Kall and Hellstrom, 1975;Manson and Palmer, 1975) and that T cells play a major role in the in vivo rejection of malignant tumors . It can also be demonstrated by in vitro tests that cytotoxic human lymphocytes can be induced in vitro to human TAA (Golub and Morton, 1975;McKhann and Jagarlamoody, 1971b;Seigler et al, 1972;Sharma and Terasaki, 1974); however the results of specific adoptive immunotherapy trials, employing human lymphocytes cocultivated in vitro with autochthonous tumor cells, have so far been relatively disappointing (Aust et al, 1970;McKhann and Jagarlamoody, 1971b;Seigler et al, 1972).…”

Different responses of lymphoid cells from tumor-bearing as compared to tumor-immunized mice when sensitized to tumor specific antigens in vitro.

“…It has recently been demonstrated that LC from normal donors can be specifically immunized to tumor antigens in vitro (5)(6)(7)(8)(9)(10). For example, Kall and Hellstrom reported that mouse lymph node cells sensitized to syngeneic sarcoma cells for 6 days in vitro were specifically cytotoxic to the appropriate target cells (8).…”

“…The present study was performed to investigate the ability of LC from tumor-bearing and tumor-excised animals to generate cytotoxic effector cells after 3 with 20 Ag/ml of mitomycin C at a cell concentration of 3 X 106 cells per ml (7). Each culture flask received 106 tumor cells.…”