Cell Attachment and Viability Study of PCL Nano-fiber Modified by Cold Atmospheric Plasma

Abstract

The field of tissue engineering is an emerging discipline which applies the basic principles of life sciences and engineering to repair and restore living tissues and organs. The purpose of this study was to investigate the effect of cold and non-thermal plasma surface modification of poly (ϵ-caprolactone) (PCL) scaffolds on fibroblast cell behavior. Nano-fiber PCL was fabricated through electrospinning technique, and some fibers were then treated by cold and non-thermal plasma. The cell-biomaterial interactions were studied by culturing the fibroblast cells on nano-fiber PCL. Scaffold biocompatibility test was assessed using an inverted microscope. The growth and proliferation of fibroblast cells on nano-fiber PCL were analyzed by MTT viability assay. Cellular attachment on the nano-fiber and their morphology were evaluated using scanning electron microscope. The result of cell culture showed that nano-fiber could support the cellular growth and proliferation by developing three-dimensional topography. The present study demonstrated that the nano-fiber surface modification with cold plasma sharply enhanced the fibroblast cell attachment. Thus, cold plasma surface modification greatly raised the bioactivity of scaffolds.

Synergistic effect of pressure cold atmospheric plasma and carboxymethyl chitosan to mesenchymal stem cell differentiation on PCL/CMC nanofibers for cartilage tissue engineering

“…Nowadays, different methods are carried out to improve or modify the surface of scaffolds, which can lead to the strengthening of the required features for tissue engineering application. One of the methods is to treat the surface of scaffold using CAP, which has advantages of needing no special solvents, no changes in fiber composition, and in the structure of fibers …”

“…One of the methods is to treat the surface of scaffold using CAP, which has advantages of needing no special solvents, no changes in fiber composition, and in the structure of fibers. 21 Plasma treatment has enhanced the hydrophilicity of the surface of scaffolds. In order to improve the characteristics of the substrate and enhance the induction of chondrogenic differentiation, plasma was used in PCL/CTS and PCL/CMC scaffolds.…”

Comparative of fibroblast and osteoblast cells adhesion on surface modified nanofibrous substrates based on polycaprolactone

“…Besides the PCL advantages in tissue engineering, inherent hydrophobicity of this polymer can result in low attachment of the cells in the first step of the cell transfer on the substrate, and consequently low rates of cell proliferation and growth (Besunder et al 2010; Atyabi et al 2016). Krishnan et al used polyvinyl alcohol/xylene blend nano-fiber produced by electrospinning which is exposed to glutaraldehyde vapor at different time intervals.…”

“…Various methods have been used to improve hydrophilicity of polymers which have led to the provision of active groups, such as (carboxyl, amino and hydroxyl) on the surface of the substrate. One of these methods is plasma surface modification treatment which helps to improve the biological properties of the polymers (Atyabi et al 2016; Desai and Singh 2004; Hourston and Lane 1993). Plasma treatment plays a vital role in improving the surface hydrophilicity of the polymer.…”

Understanding the impact of crosslinked PCL/PEG/GelMA electrospun nanofibers on bactericidal activity

“…19-22 Due date, several strategies have been applied to combine PCL with other polymers to obtain partial or total hydrophilicity to open numerous possibilities for biomedical purposes. 23-30 Furthermore, PEG is biocompatible, hydrophilic and has the ability to reduce protein adsorption. 31 However, it still has limits in that it cannot be used alone to produce mats, but rather needs to be combined with other polymers.…”