Comparative of fibroblast and osteoblast cells adhesion on surface modified nanofibrous substrates based on polycaprolactone

Abstract

One of the determinant factors for successful bioengineering is to achieve appropriate nano-topography and three-dimensional substrate. In this research, polycaprolactone (PCL) nano-fibrous mat with different roughness modified with O2 plasma was fabricated via electrospinning. The purpose of this study was to evaluate the effect of plasma modification along with surface nano-topography of mats on the quality of human fibroblast (HDFs) and osteoblast cells (OSTs)-substrate interaction. Surface properties were studied using scanning electron microscopy (SEM), atomic force microscopy (AFM), contact angle, Fourier-transformation infrared spectroscopy. We evaluated mechanical properties of fabricated mats by tensile test. The viability and proliferation of HDFs and OSTs on the substrates were followed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT). Mineralization of the substrate was determined by alizarin red staining method and calcium content of OSTs was determined by calcium content kit. Cells morphology was studied by SEM analysis. The results revealed that the plasma-treated electrospun nano-fibrous substrate with higher roughness was an excellent designed substrate. A bioactive topography for stimulating proliferation of HDFs and OSTs is to accelerate the latter’s differentiation time. Therefore, the PCL substrate with high density and major nano-topography were considered as a bio-functional and elegant bio-substrate for tissue regeneration applications.

Electrospun Combination of Peppermint Oil and Copper Sulphate with Conducive Physico-Chemical properties for Wound Dressing Applications

“…The smooth surface will have a smaller fiber diameter which would be suitable for the fibroblast cells to adhere and grow [18,31]. On other hand, Sharifi et al reported that surface modified polycaprolactone fibrous membrane with increased surface roughness also favors the improved adhesion and growth of fibroblast cells [33]. The influence of surface roughness on the cellular response is still unclear and the various physico-chemical properties of the manufactured composites might have a role in the cell adhesion behavior.…”

Biphasic, tough composite core/shell PCL/PVA‐GEL nanofibers for biomedical application

“…Higher contact angle shows a low level of hydrophilicity of the substrates . Better cellular proliferation may be pertained to the hydrophilicity which promotes the rate of cellular proliferation …”

“…35 Better cellular proliferation may be pertained to the hydrophilicity which promotes the rate of cellular proliferation. 36 Observation of the WCA on the surface of core/shell nanofibers mats is useful, because of the difference in hydrophilicity of the PCL (hydrophobic) as core material and PVA-GEL (hydrophilic) as shell material. Figure 4 shows the WCA of crosslinked PCL/PVA-GEL core/shell nanofibers as well as PCL and crosslinked PVA-GEL nanofibers.…”

Engineered Electrospun Polyurethane Composite Patch Combined with Bi-functional Components Rendering High Strength for Cardiac Tissue Engineering

“…Cell behavior is complex phenomenon and it was influenced by various properties. The properties include fiber diameter, pore size, wettability, roughness, surface chemistry, surface energy, additives, and degradation [25,34,42,43,44,45,46,47,48]. It was reported that the smaller fiber diameter morphology will favors the enhanced cellular response [49].…”