Biphasic, tough composite core/shell PCL/PVA‐GEL nanofibers for biomedical application

Abstract

Emulsion electrospinning using natural and synthetic polymers, including two dissimilar materials is a promising technique for nanofibers fabrication in a core/shell configuration for tissue engineering, controlled or sustained drug delivery and dressing applications. In this study, we designed and fabricated core/shell nanofibers based on polycaprolactone (PCL) as core material and poly(vinyl alcohol) (PVA)‐gelatin (GEL) blend as shell materials (PCL/PVA‐GEL) to achieve high mechanical properties, good cell growth, and proliferation via emulsion electrospinning. The effect of water to acetic acid ratio in the solvent system (8:2, 7:3, 6:4, 5:5) and also type and concentration (3, 5, 7 w/v %) of surfactant on emulsion stability and nanofibers morphology were investigated. The emulsion containing 2% Tween80 and 1% Span60 as surfactants were selected by considering the stability of emulsion and uniform fiber morphology. In the tensile strength and elongation at break, 53 and 8% increase in the crosslinked wet state of the PCL/PVA‐GEL nanofibers compared with PVA‐GEL nanofibers were observed respectively. The cell culture results indicated that the PCL/PVA‐GEL nanofibers surface has presented suitable interaction with fibroblast cells and cells attached and proliferated well on the fabricated substrate within 24 and 48 hours and also would be a good candidate for biomedical applications. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020, 137, 48713.

Effect of Ionic and Non-Ionic Surfactant on Bovine Serum Albumin Encapsulation and Biological Properties of Emulsion-Electrospun Fibers

“…The decrease in the viscosity of the T4 sample indicates a decrease in the interfacial energy between the droplets due to, for example, coalescence or flocculation. This decrease indirectly indicates that the emulsion started to lose stability [ 47 ]. One hour after preparation, this drop in viscosity is more likely to be due to the decomposition of the micelles than to the miscibility of the water and oil phases or the volume fraction of the water phase.…”

“…Akbarzadeh et al obtained PCL/PVA-GEL core/shell nanofibers, which induced adhesion of the L929 fibroblasts. Biological studies showed high cell viability (above 80%) after 24 h and 48 h incubation, spindle-like morphology, and no signs of damage (cells debris) [ 47 ]. Basar et al, who also conducted research on the same type of cells, reported a positive effect of the emulsion electrospun PCL/gelatin fibers containing ketoprofen on the viability ant phenotype of the L929 fibroblasts [ 42 ].…”

Decellularized placental sponge as an excellent carrier for human mesenchymal stem cells and management of full-thickness skin wounds: an in vitro and in vivo study

“…The porosity of DPS was determined using the liquid displacement technique [27]. The scaffold (W d , dry weight) was submerged in ethanol for 30 min and the weight of wet samples was calculated W 1 .…”

Recent applications of electrical, centrifugal, and pressurised emerging technologies for fibrous structure engineering in drug delivery, regenerative medicine and theranostics

“…PCL has also been combined with various polymers to create blended fibrous scaffolds with enhanced mechanical properties and cell attachment capabilities. Akbarzadeh et al fabricated a biphasic fibrous matrix composed of PCL, PVA and gelatin which demonstrated fibroblast interaction and enhanced cell proliferation within 24 hours [441] whilst Ghasemkhah et al developed gelatin/PCL scaffolds which proved to be promising protein delivery systems to aid in the tissue regeneration process [442].…”