CD3-CD20–positive nodal lymphoma with cross-lineage rearrangement in a dog

Nicoletti, Arturo; Aresu, Luca; Marino, Mariacatia; Massaro, Maria; Martignani, Eugenio; Caporali, Elisa; Capuccini, Silvia; Bonfanti, Ugo; Gola, Cecilia

doi:10.1177/1040638720963132

Cited by 4 publications

(8 citation statements)

References 14 publications

(15 reference statements)

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“…Co-expression of CD3 and CD20 confirmed by immunohistochemistry is rare and has been described in only a few cases of canine T-cell lymphomas, such as cutaneous, intestinal, nodal, and peripheral lymphomas with infiltration of the heart and peripheral nerves [ 9 , 10 , 11 , 20 ]. Double lymphoid labelling has also been described in a cat, but in a B-cell lymphoma with expression of CD3, identified by clonality tests [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…With these methods, it is expected that most neoplastic cells from intestinal lymphoid tumors will express either one of the T- or B-cell markers, but not both. However, there are already some reports of co-expression of CD3 and CD20 in canine lymphomas, including three cases of intestinal T-cell lymphomas associated with type 1 EATL [ 9 , 10 , 11 ]. These findings reinforce the importance of PCR for antigen receptor rearrangement (PARR) for a definitive diagnosis of lymphoid cells in intestinal neoplasms [ 1 , 2 , 6 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Co-Expression of T- and B-Cell Markers in a Canine Intestinal Lymphoma: A Case Report

Peleteiro

Carvalho²,

Leal

et al. 2022

Animals

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An 8-year-old female neutered Labrador retriever was presented for a second opinion consultation due to vomiting and lethargy, having failed to respond to symptomatic therapy. Blood analysis revealed hyperbilirubinemia and hypoalbuminemia, associated with hypocobalaminemia. An abdominal ultrasound identified diffused bowel thickening and hypoechoic hepatomegaly. An ultrasound-guided liver fine-needle aspiration was performed for cytology and also for cell block immunocytochemistry. Gastric and duodenal biopsies were collected by gastroduodenoscopy. Liver cytology showed numerous lymphocytes, suggesting lymphoma at the hepatic infiltration stage, and immunocytochemistry in the cell block of the hepatic aspirate indicated co-expression of CD3 and CD20 in the lymphoid cells present. The histopathology of gastric and duodenal biopsies supported the hypothesis of gastrointestinal lymphoma due to heavy lymphoid infiltration of the gastric epithelium and intestinal mucosa, including the villi. Concurrent immunohistochemistry was performed using CD3, CD20, PAX5, and CD79αcy antibodies. Immunomarking was positive for CD3 and CD20, which overlapped populations of lymphoid cells, and was negative for all other antibodies. In the clonality test, lymphocyte co-expression of CD3 and CD20 was confirmed by monoclonal rearrangement of T-cell gamma receptors. The final diagnosis was type 2 enteropathy-associated T-cell lymphoma with hepatic infiltration. Co-expression was examined in conjunction with the PARR result in the presence of T-cell monoclonal rearrangement.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Co-Expression of T- and B-Cell Markers in a Canine Intestinal Lymphoma: A Case Report

Peleteiro

Carvalho²,

Leal

et al. 2022

Animals

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“…Clonality for B/T cell lymphoma is rarely observed; however, some authors have reported a cross-line between T and B lymphocytes, positive for both clones by immunohistochemistry and PCR, the last technique being performed to confirm lymphoid clonality [9]. This is because the test performed using PCR is considered more sensitive for detection.…”

Section: Discussionmentioning

confidence: 99%

“…This is because the test performed using PCR is considered more sensitive for detection. One of the hypotheses raised for this type of lymphoma is that a chromosomal mutation occurs, causing the exchange of phenotypic receptors; that is, a lymphoma starts as B cells, and in contact with exogenous factors, it can undergo changes and express a T phenotype, and clonal detection may occur for techniques, such as immunohistochemistry or both sets of primers by PCR [7,9].…”

Section: Discussionmentioning

confidence: 99%

Clonality Test by PCR - PARR in Real Time of Canine Lymphomas

Gonçalves

Souza

Albuquerque

et al. 2021

Acta Scientiae. Vet.

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Background: Lymphoma is a neoplasm of hematopoietic origin that affects canines. The proper establishment of prognosis and rapid institution of treatment are essential for a better quality of life, and immunophenotyping is one of the tools used for this purpose. The objective of this study was to perform a clonality test for immunophenotypic characterization of canine lymphomas using the polymerase chain reaction (PCR) for antigen receptor rearrangements (PARR) technique in real-time from samples fixed in formalin and embedded in paraffin.Materials, Methods & Results: The 23 analyzed samples were fixed in formalin and embedded in paraffin canine lymphoma from the Collection Laboratory of Histopathology of the Animal Pathology Area of the Departament of Veterinary Medicine - Federal Rural University of Pernambuco UFRPE. Samples were processed, their DNA was extracted, quantified, diluted, and standardized at a concentration of 50 ng/µL. After extraction, all samples were subjected to conventional PCR for endogenous control (detection of the IgM target region), in which the extracted DNA was amplified in a final volume of 25 µL. The 128 bp amplified product was detected by 1.5% agarose gel electrophoresis. Of the 23 samples analyzed for the detection of the conserved region referring to the endogenous gene, 91.30% (21/23) amplified the conserved region Cµ by conventional PCR, and two samples 8.70% (2/23) were negative. Endogenous control positive samples were subjected to real-time PCR-PARR for detection of IgH Major (LB), IgH Minor (LB), and TCRγ (LT) target regions. All reactions were performed in duplicate to reduce the risk of false-positive or false-negative results due to technical errors. Samples previously confirmed by immunohistochemistry were used as positive controls for T cell and B cell lymphoma, and MilliQ water was used as a negative control. After amplification, the melting curve gradually increased the temperature by 1C/5 s to 95C during continuous fluorescence monitoring. Of the 21 samples analyzed, 100.00% (21/21) demonstrated clonal amplification. Of these, 57.15% (12/21) were positive for phenotype B, and 42.85% (9/21) were positive for phenotype T.Discussion: Due to the importance of researching and confirming samples from files fixed and embedded in paraffin samples in laboratories, PCR-PARR is a good tool for this purpose. In the present study, real-time PCR analysis demonstrated greater sensitivity in the characterization of the immunophenotype of lymphomas from old samples fixed in formalin and embedded in paraffin. The temperature of melting curve analysismay vary depending on the amount of DNA and its quality. In the present study, it was found that the average melting temperature in the samples varied between ± 3C when compared to that in the control sample for LB and LT, 83.5C and 80C, respectively: in the literature, there is a relative difference in this temperature, which may vary up to 4C. Real-time PCR-PARR was satisfactory in the characterization of the immunophenotype of canine lymphomas from formalin-fixed and paraffin-embedded samples; therefore, its use is recommended for both retrospective studies. The use of PCR-PARR associated with histopathological and/or cytopathological examination in cases of canine lymphomas strongly helps pathologists, provide a safe establishment of the immunophenotype, minimize errors, and optimize the diagnosis, thus directly contributing to the establishment of the prognosis.Keywords: immunophenotyping, lymphoproliferative disease, real-time PCR, TCRγ, IgH.

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“…The PCR for antigen receptor rearrangement analysis (PARR) was performed on the remaining specimens of LN aspirate, PB, and BM, according to protocols published previously. 12 Clonal cross-rearrangement of both B-and T-cell receptors (BCR and TCR) was retrieved on all 3 matrices, and peaks were superimposable (Suppl. Fig.…”

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confidence: 99%

Tumor staging in a Beagle dog with concomitant large B-cell lymphoma and T-cell acute lymphoblastic leukemia

Ferrari¹,

Cozzi

Aresu

et al. 2021

J VET Diagn Invest

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An 8-y-old spayed female Beagle dog was presented with peripheral lymphadenomegaly. Lymph node cytology and flow cytometry led to the diagnosis of large B-cell lymphoma (LBCL). We detected minimal percentages of LBCL cells in peripheral blood and bone marrow samples. However, a monomorphic population of neoplastic cells different from those found in the lymph node was found in the bone marrow. T-cell acute lymphoblastic leukemia was suspected based on flow cytometric immunophenotyping. PCR for antigen receptor rearrangement (PARR) revealed clonal rearrangement of both B-cell and T-cell receptors, and the presence of both neoplastic clones in the lymph node, peripheral blood, and bone marrow. The dog was treated with multi-agent chemotherapy but died 46 d following diagnosis. Tumor staging and patient classification are needed to accurately establish a prognosis and select the most appropriate therapeutic protocol.

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CD3-CD20–positive nodal lymphoma with cross-lineage rearrangement in a dog

Cited by 4 publications

References 14 publications

Co-Expression of T- and B-Cell Markers in a Canine Intestinal Lymphoma: A Case Report

Co-Expression of T- and B-Cell Markers in a Canine Intestinal Lymphoma: A Case Report

Clonality Test by PCR - PARR in Real Time of Canine Lymphomas

Tumor staging in a Beagle dog with concomitant large B-cell lymphoma and T-cell acute lymphoblastic leukemia

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