Causes of breakage and disruption in a homogeniser

Abstract

Abstract:Many authors have written in the past regarding the exact causes of breakage and disruption in a high pressure homogeniser, but there has been little agreement. This paper investigates some of the most likely causes of the rupture of the walls of unicellular organisms and offers suggestions obtained from various papers and work carried out.

Effect of homogenization and ultrasonication on the physical properties of insoluble wheat bran fibres

“…As shown in Table 1, the particle size of HIU, HPH, and HPH+HIU samples decreased with the increase in the initial IDF concentration. Clarke et al (2010) have recently reviewed the causes of the breakup of particles in the high-pressure homogenization process and concluded that shear stress encountered by a fluid is the main cause of the particle breakup during HPH. An increase in the concentration of IDF increases the viscosity of the suspension (Elleuch et al, 2011), which increases the shear stress during HPH.…”

Underlying factors to consider in improving energy yield from biomass source through yeast use on high-pressure homogenizer (hph)

“…Before setting up the assay, the protein to be used as a standard is decided upon and the range which it is likely to be sensitive is determined. As sensitivity vary from one reagent batch to another, immunoglobulin G (IgG) is frequently used as a standard in Bradford assay the same way as bovine serum albumin (BSA) [35]. With the BSA solutions, 0.1M phosphate buffer, pH 7.0 of each protein to 0.5, 1, 2, 4 and 5 mg/ml were prepared to dilutions.…”

“…Other underlying factors of considerable importance which have also been analyzed; authors have suggested a correlation between cell wall structure and high-pressure resistance between the microorganisms and HPH. This indicates that high-pressure homogenization kills vegetative bacteria mainly through mechanical destruction of the cell integrity, caused by the spatial pressure and velocity gradients, turbulence, impingement [30,31] and cavitation [32,33] Disruption of yeast or other microbial organisms is a key step towards the isolation and purification of many biotechnological products that are present in the interior of cells of the cell walls of microorganisms [34] and it was reported in the concept presented by Clarke et al [35] as example of breaking the walls of yeast cells. These were found to be between 5-10 microns in sizes and not limited to just yeast but also applicable to other unicellular micro-organisms of different sizes.…”

Yeast cell disruption strategies for recovery of intracellular bio-active compounds — A review

“…Mechanical methods including bead mill (Woodrow, & Quirk, 1982) high-pressure homogenization (Clarke, Prescott, & Khan, 2010),and ultrasonication (Chemat, Huma, & Khan, 2011) are most widely used to achieve microbial cell disruption for intracellular product release at an industrial scale (Harrison, 1991;Middelberg, 1995). These methods result in considerable cell breakage and high recovery of bio-active compounds.…”