Yeast cell disruption strategies for recovery of intracellular bio-active compounds — A review

Liú, Dan; Ding, Lijun; Sun, Jianxia; Boussetta, Nadia; Vorobiev, Eugène

doi:10.1016/j.ifset.2016.06.017

Cited by 109 publications

(99 citation statements)

References 114 publications

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“…Generally, the yeast cell wall is composed by thick and rigid layer of complex carbohydrates and glycoproteins with high mechanical strength and chemical resistance . In consequence, the cell wall disruption is the most challenging part of lipid extraction process from yeast cells, since lipids are located inside the cell . Numerous disruption methods including physical, chemical and enzymatic have been developed to achieve selective release of biomolecules .…”

Section: Introductionmentioning

confidence: 99%

Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

Karim

Yousuf

Islam

et al. 2018

Biotechnology Progress

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The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min where the distance between electrodes was maintained at 2, 4, and 6 cm. Colony forming units (CFU) were counted to observe the cell viability under the high voltage electric field. The forces of the pulsing electric field caused significant damage to the cell wall of L. starkeyi and the disruption of microbial cells was visualized by field emission scanning electron microscopic (FESEM) image. After breaking the cell wall, lipid was extracted and measured to assess the efficiency of EP over other techniques. The extent of cell inactivation was up to 95% when the electrodes were placed at the distance of 2 cm, which provided high treatment intensity (36.7 kWh m ). At this condition, maximum lipid (63 mg g ) was extracted when the biomass was treated for 10 min. During the comparison, EP could extract 31.88% lipid while the amount was 11.89% for ultrasonic and 16.8% for Fenton's reagent. The results recommend that the EP is a promising technique for lowering the time and solvent usage for lipid extraction from microbial biomass. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:838-845, 2018.

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Section: Introductionmentioning

confidence: 99%

Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

Karim

Yousuf

Islam

et al. 2018

Biotechnology Progress

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“…On the other hand, non‐mechanical techniques (electrical, physical, chemical and enzymatic) are more selective and gentler. Nonetheless, these methods are often limited to laboratory scale owing to the low efficiency of physical methods (osmotic shock and thermolysis) and economic constraints …”

Section: Introductionmentioning

confidence: 99%

Extraction and purification of violacein from Yarrowia lipolytica cells using aqueous solutions of surfactants

Kholany

Trébulle

Martins

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND The demand for colorants from natural bio‐based sources is increasing. Violacein is a natural purple‐blue hydrophobic pigment with interesting bioactivity whose expression in genetically modified Yarrowia lipolytica production was successfully achieved. RESULTS In this work, several surfactants were tested in the extraction of violacein from Y. lipolytica cells, and the operational conditions were optimized to maximize the extraction yield. After the optimization, the purification of violacein using aqueous biphasic systems (ABS) composed of Tween 20 and cholinium‐based ionic liquids (ILs) was pursued. The ABS were characterized and applied in the separation of violacein from contaminant proteins, reaching a maximum selectivity of 155 with violacein being fully concentrated in the Tween 20‐rich phase, with 80% of the contaminant proteins present in the extract being removed. CONCLUSION This work led to a conceptual downstream process based on a first step of (solid–liquid) extraction and a second step addressing the separation of violacein from contaminant proteins using ABS. © 2019 Society of Chemical Industry

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“…Mechanical methods, on the other hand, are promising as these can be scaled up with minimum or no pre-treatment/chemical additives and can be used in batch or continuous modes. Bead milling is one of the most preferable mechanical cell lysis methods at the industrial scale due to its ease of operation, controllability, and ability to load concentrated cell slurry [17,19]. This method has been a subject of continuing research in batch and continuous modes [19,20].…”

Section: Introductionmentioning

confidence: 99%

“…Bead milling is one of the most preferable mechanical cell lysis methods at the industrial scale due to its ease of operation, controllability, and ability to load concentrated cell slurry [17,19]. This method has been a subject of continuing research in batch and continuous modes [19,20]. The common principle of a bead mill is that the cells are subjected to high stress produced by abrasion during rapid agitation with glass beads, resulting in the breaking of the membrane and cell wall, releasing all intracellular components [21].…”

Section: Introductionmentioning

confidence: 99%

Efficient Disruption of Escherichia coli for Plasmid DNA Recovery in a Bead Mill

et al. 2017

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Featured Application: In general, the alkaline lysis is more acceptable than the mechanical lysis for pDNA recovery due to the risk of product degradation when the latter is used. In this work, the experimental design allowed us to explore mechanical disruption conditions under which such an effect was minimized. The application of this technique is important for novel pDNA-vaccine process development, since bead milling is one of the most preferable mechanical cell lysis methods on an industrial scale due to its scalability, ease of operation, controllability, and ability to load concentrated cell slurry. Abstract:The release kinetics of pDNA in a bead mill was studied. Samples taken during the process were analyzed to determine total pDNA (pDNA(t)) and supercoiled pDNA (pDNA(sc)) concentration. In order to identify important variables of the process and to develop an empirical model for optimal pDNA(t) and pDNA(sc) release, a two level 2 3 factorial design was used with variables: mill frequency, cell concentration, and bead size. The results were analyzed by response surface methodology. The optimized conditions for pDNA(t) yield 13.26 mg/g dcw (93.41% recovery), with a mill frequency of 30 Hz, a bead size of 0.10-0.25 mm, and a cell concentration of 20 g wcw/L. However, the optimized conditions for pDNA(sc) yield 7.65 mg/g dcw (92.05% recovery), with a mill frequency of 15 Hz, a bead size of 0.10-0.25 mm, and a cell concentration of 10 g wcw/L. Cell disruption in a bead mill was proved efficient for the release of pDNA(t) and pDNA(sc) compared to the alkaline treatment. The results obtained suggest a compromise between pDNA(sc) purity and recuperation in the process development.

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Yeast cell disruption strategies for recovery of intracellular bio-active compounds — A review

Cited by 109 publications

References 114 publications

Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

Extraction and purification of violacein from Yarrowia lipolytica cells using aqueous solutions of surfactants

Efficient Disruption of Escherichia coli for Plasmid DNA Recovery in a Bead Mill

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