Catabolism of Nitrogenous Compounds by Pseudomonas

Gaby, W. L.; Logan, C.; Whitaker, S.

doi:10.1099/00221287-28-2-379

Cited by 4 publications

(2 citation statements)

References 4 publications

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“…Production of alkalinity in glucose-amino acid media was observed in Pseudomonas species (Rhodes, 1959;Gaby, Logan, and Whitaker, 1962), and probably occurs in other species of this physiological type as well.…”

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confidence: 85%

FACTORS INFLUENCING GROWTH AND POLYSACCHARIDE FORMATION BY STRAINS OF CHROMOBACTERIUM VIOLACEUM

Corpe

1964

J Bacteriol

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strains elaborate a fibrous exopolysaccharide that forms a matrix in which growing cells are embedded. Stable, nongelatinous variants arising from gelatinous cultures were of two types: (i) those that had lost the ability to produce polysaccharide, and (ii) those that had lost the capacity to bind the polymer into a mnatrix. Gelatinous strains growing in Tryptone did not produce a matrix, but rather elaborated polysaccharide free into the medium. The effect of Tryptone was not one of selection of nongelatinous variants. Growth and polysaccharide synthesis in a defined medium were greatest when amino acids were substituted for ammonia as a nitrogen source. Best growth and polysaccharide yield occurred when the ratio of carbohydrate to nitrogen was in the order of 10:1. Depolymerization of formed polymer occurred in cultures grown with Casamino Acids as the nitrogen source. Calcium ion stimulated polysaccharide formation. Iron, although stimulating growth, inhibited polysaccharide formation.

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confidence: 85%

FACTORS INFLUENCING GROWTH AND POLYSACCHARIDE FORMATION BY STRAINS OF CHROMOBACTERIUM VIOLACEUM

Corpe

1964

J Bacteriol

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“…We also observed that, at the end of the experiment, the extracellular pH of both, acidic and alkaline cultures was modified, converging in a pH around 8.6. The culture media alkalization was previously observed when ammonia was released due to peptones’ use as carbon and nitrogen sources [39], in this case from LB medium. Interestingly, cultures inoculated under alkaline conditions showed a decrease in media pH at the end of the assays.…”

Section: Discussionmentioning

confidence: 99%

Unraveling the effects of polyhydroxyalkanoates accumulation inPseudomonas extremaustralisgrowth and survival under different pH conditions

Brito,

López,

Raiger Iustman

2024

Preprint

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Polyhydroxyalkanoates (PHAs) are intracellular polymers that enhance bacterial fitness against various environmental stressors.Pseudomonas extremaustralis14-3b is an Antarctic bacterium capable of accumulating, short-chain-length PHAs (sclPHAs), composed of C3–C5 monomers, as well as medium-chain-length PHAs (mclPHAs) containing ≥C6 monomers. Since pH changes are pivotal in bacterial physiology, influencing microbial growth and metabolic processes, we propose that accumulated PHA increasesP. extremaustralisfitness to cope with pH changes. To test this, we analyzed the production of sclPHA and mclPHA at different pH levels and its effect on bacterial survival against pH stress.P. extremaustraliswas able to grow and accumulate PHA when the culture media pH ranged from 6.0 to 9.5, showing a marked loss of viability outside this range. Additionally, based on the analysis of different PHA-deficient mutants, we found that when exposed to both acidic and alkaline conditions, sclPHA and mclPHA conferred different protection against pH stress, with sclPHA making the main contribution. These results highlight the importance of PHA in supporting survival in pH-stressful environments.

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Transfer and expression of Klebsiella nif genes in Alcaligenes faecalis, a nitrogen-fixing bacterium associated with rice root

Chen

1983

Plasmid

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Catabolism of Nitrogenous Compounds by Pseudomonas

Cited by 4 publications

References 4 publications

FACTORS INFLUENCING GROWTH AND POLYSACCHARIDE FORMATION BY STRAINS OF CHROMOBACTERIUM VIOLACEUM

FACTORS INFLUENCING GROWTH AND POLYSACCHARIDE FORMATION BY STRAINS OF CHROMOBACTERIUM VIOLACEUM

Unraveling the effects of polyhydroxyalkanoates accumulation inPseudomonas extremaustralisgrowth and survival under different pH conditions

Transfer and expression of Klebsiella nif genes in Alcaligenes faecalis, a nitrogen-fixing bacterium associated with rice root

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