FACTORS INFLUENCING GROWTH AND POLYSACCHARIDE FORMATION BY STRAINS OF
            <i>CHROMOBACTERIUM VIOLACEUM</i>

Corpe, William A.

doi:10.1128/jb.88.5.1433-1441.1964

Cited by 25 publications

(4 citation statements)

References 9 publications

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“…The mechanisms of Ca 2+ uptake and partitioning in E. huxleyi may also differ from those in other coccolithophores (Sviben et al ., ; Gal et al ., ). The absence of a paired‐cell phenotype in C. braarudii in low Ca 2+ may also relate to the influence of low external Ca 2+ on the physical properties of the extracellular polysaccharides, as many algal polysaccharides, such as pectins and alginates, are cross‐linked by Ca 2+ and exhibit vastly different properties at lower Ca 2+ concentrations (Corpe, ; Haug, ; Matoh & Kobayashi, ; Domozych et al ., ).…”

Section: Discussionmentioning

confidence: 99%

The requirement for calcification differs between ecologically important coccolithophore species

et al. 2018

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Summary Coccolithophores are globally distributed unicellular marine algae that are characterized by their covering of calcite coccoliths. Calcification by coccolithophores contributes significantly to global biogeochemical cycles. However, the physiological requirement for calcification remains poorly understood as non‐calcifying strains of some commonly used model species, such as Emiliania huxleyi, grow normally in laboratory culture.To determine whether the requirement for calcification differs between coccolithophore species, we utilized multiple independent methodologies to disrupt calcification in two important species of coccolithophore: E. huxleyi and Coccolithus braarudii. We investigated their physiological response and used time‐lapse imaging to visualize the processes of calcification and cell division in individual cells.Disruption of calcification resulted in major growth defects in C. braarudii, but not in E. huxleyi. We found no evidence that calcification supports photosynthesis in C. braarudii, but showed that an inability to maintain an intact coccosphere results in cell cycle arrest.We found that C. braarudii is very different from E. huxleyi as it exhibits an obligate requirement for calcification. The identification of a growth defect in C. braarudii resulting from disruption of the coccosphere may be important in considering their response to future changes in ocean carbonate chemistry.

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Section: Discussionmentioning

confidence: 99%

The requirement for calcification differs between ecologically important coccolithophore species

et al. 2018

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“…Since cations have been shown to influence surface adhesion of cells which elaborate acidic extracellular polymeric substances (EPS) that extend between the cell surface and the substratum (Fletcher & Floodgate, 1973;1976), other studies have explored the influence of cations on the biosynthesis and behavior of these substances. Several studies have demonstrated the importance of Mg ++ for maximum EPS production (Corpe, 1964;Tempest et ah, 1965). In other studies, however, calcium limitation (reduction of CaCl 2 from lOmgl" 1 to 4mgl~1) was found to have little effect on the production of surface-associated carbohydrate and adhesion over a 15 h period to glass substrata by cells of two Gram-negative bacteria isolated from a freshwater stream (Allison & Sutherland, 1987).…”

Section: Influence Of Specific Cations On the Production And Behavior...mentioning

confidence: 99%

Influence of calcium and other cations on surface adhesion of bacteria and diatoms: A review

Geesey¹,

2000

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Association with a surface is an important aspect of survival for microorganisms in natural and manmade environments/Both bacteria and diatoms are involved in such associations. In many cases, this leads to surface fouling, which often results in surface deterioration and mechanical failure in industrial systems. We now know that microorganisms exploit many strategies to establish associations with surfaces. As in the case of other cellular processes, calcium ions seem to play an important role in adhesion of cells to surfaces. Calcium is involved in non-specific interactions such as neutralization of the electrical double layer between cell and substratum surface as well as specific adhesive interactions that cannot be replaced by other cations. The unique properties of calcium ions promote both specific and non-specific interactions with protein and polysaccha-ride adhesin molecules at the cell surface. As important, but less well understood, calcium ions also influence the way microbial cells interact with different substrata.

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“…The absence of this micronutrient might, however, be a reason for the lack of repeatability of the DMB-P min , as growth failed after several re-inoculations in the medium. Use of this element at high concentration might be toxic or inhibitory, and has been reported to have a negative effect on cell growth [ 24 , 25 ]. Bacteria, in general, can take up ferrous iron (Fe 2+ ) via a ferrous ion internalization system [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Medium development and production of carotenoids and exopolysaccharides by the extremophile Rhodothermus marinus DSM16675 in glucose-based defined media

et al. 2022

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Background The marine thermophilic bacterium Rhodothermus marinus can degrade many polysaccharides which makes it interesting as a future cell factory. Progress using this bacterium has, however, been hampered by limited knowledge on media and conditions for biomass production, often resulting in low cell yields and low productivity, highlighting the need to develop conditions that allow studies of the microbe on molecular level. This study presents development of defined conditions that support growth, combined with evaluation of production of carotenoids and exopolysaccharides (EPSs) by R. marinus strain DSM 16675. Results Two defined media were initially prepared: one including a low addition of yeast extract (modified Wolfe’s medium) and one based on specific components (defined medium base, DMB) to which two amino acids (N and Q), were added. Cultivation trials of R. marinus DSM 16675 in shake flasks, resulted in maximum cell densities (OD620 nm) of 2.36 ± 0.057, cell dry weight (CDW) 1.2 ± 0.14 mg/L, total carotenoids 0.59 × 10–3 mg/L, and EPSs 1.72 ± 0.03 mg/L using 2 g/L glucose in DMB. In Wolfe’s medium (supplemented by 0.05 g/L yeast extract and 2.5 g/L glucose), maximum OD620 nm was 2.07 ± 0.05, CDW 1.05 ± 0.07 mg/L, total carotenoids 0.39 × 10–3 mg/L, and EPSs 1.74 ± 0.2 mg/L. Growth trials at 5 g/L glucose in these media either failed or resulted in incomplete substrate utilization. To improve reproducibility and increase substrate utilization, a screening of macroelements (e.g. phosphate) in DMB, was combined with use of trace elements and vitamins of the modified Wolfe’s medium. The resulting defined minimal R. marinus medium, (DRM), allowed reproducible cultivations to a final OD620nm of 6.6 ± 0.05, CDW 2.85 ± 0.07 mg/L, a maximum specific growth rate (µmax) of 0.26 h−1, total carotenoids 0.77 × 10–3 mg/L and EPSs 3.4 ± 0.17 mg/L in cultivations supplemented with up to 5 g/L glucose. Conclusion A minimal defined medium (DRM) was designed that resulted in reproducible growth and an almost doubled formation of both total carotenoids and EPSs. Such defined conditions, are necessary for systematic studies of metabolic pathways, to determine the specific requirements for growth and fully characterize metabolite production.

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FACTORS INFLUENCING GROWTH AND POLYSACCHARIDE FORMATION BY STRAINS OF CHROMOBACTERIUM VIOLACEUM

Cited by 25 publications

References 9 publications

The requirement for calcification differs between ecologically important coccolithophore species

The requirement for calcification differs between ecologically important coccolithophore species

Influence of calcium and other cations on surface adhesion of bacteria and diatoms: A review

Medium development and production of carotenoids and exopolysaccharides by the extremophile Rhodothermus marinus DSM16675 in glucose-based defined media

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