Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules

Isaeva, E. V.; Бекетов, Е. Е.; Demyashkin, Grigory; Yakovleva, N. D.; Arguchinskaya, Nadezhda V.; Kisel, Anastas A.; Lagoda, Tatiana S.; Malakhov, Egor P.; Smirnova, Anna N.; Петриев, В. М.; Eremin, Petr S.; Osidak, E. O.; Domogatsky, S.P.; Иванов, С. А.; Shegay, Peter; Каприн, А. Д.

doi:10.3390/ijms23052703

Cited by 13 publications

(14 citation statements)

References 64 publications

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“…Scaffolds with dECM are currently being investigated for use in the treatment of osteochondral defects and for the regeneration of cartilage [ 1 , 3 , 34 , 35 , 36 , 37 , 38 , 39 ]. While decellularization techniques have been developed and used to create a biological ECM from cells in culture, tissues, and organs, it is possible that material within the scaffold could have an adverse effect if not removed.…”

Section: Decellularization As a Strategy To Create Cartilage Biomater...mentioning

confidence: 99%

Advances in Cartilage Tissue Engineering Using Bioinks with Decellularized Cartilage and Three-Dimensional Printing

Stone

Reeck

Oxford

2023

IJMS

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Osteoarthritis, a chronic, debilitating, and painful disease, is one of the leading causes of disability and socioeconomic burden, with an estimated 250 million people affected worldwide. Currently, there is no cure for osteoarthritis and treatments for joint disease require improvements. To address the challenge of improving cartilage repair and regeneration, three-dimensional (3D) printing for tissue engineering purposes has been developed. In this review, emerging technologies are presented with an overview of bioprinting, cartilage structure, current treatment options, decellularization, bioinks, and recent progress in the field of decellularized extracellular matrix (dECM)–bioink composites is discussed. The optimization of tissue engineering approaches using 3D-bioprinted biological scaffolds with dECM incorporated to create novel bioinks is an innovative strategy to promote cartilage repair and regeneration. Challenges and future directions that may lead to innovative improvements to currently available treatments for cartilage regeneration are presented.

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Section: Decellularization As a Strategy To Create Cartilage Biomater...mentioning

confidence: 99%

Advances in Cartilage Tissue Engineering Using Bioinks with Decellularized Cartilage and Three-Dimensional Printing

Stone

Reeck

Oxford

2023

IJMS

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“…3D printing is a promising technique for the formation of personalized grafts for cartilage and bone regeneration using hydrogels, bone chips, laser-sintered titanium [ 93 , 94 , 95 , 96 ]. Traditionally, 3D bioprinting involves cells cultured in vitro.…”

Section: Methods Of Intraoperative Cell Seeding On Scaffoldsmentioning

confidence: 99%

Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ

et al. 2022

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Transfer of regenerative approaches into clinical practice is limited by strict legal regulation of in vitro expanded cells and risks associated with substantial manipulations. Isolation of cells for the enrichment of bone grafts directly in the Operating Room appears to be a promising solution for the translation of biomedical technologies into clinical practice. These intraoperative approaches could be generally characterized as a joint concept of tissue engineering in situ. Our review covers techniques of intraoperative cell isolation and seeding for the creation of tissue-engineered grafts in situ, that is, directly in the Operating Room. Up-to-date, the clinical use of tissue-engineered grafts created in vitro remains a highly inaccessible option. Fortunately, intraoperative tissue engineering in situ is already available for patients who need advanced treatment modalities.

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“…Cartilage-derived dECMs are commonly used for cartilage tissue regeneration and repair. Isaeva EV et al created a bioink that contains BMSCs, 4% collagen, and 2.5% dECM particles produced from articular cartilage [ 142 ]. Both cellular osteogenesis and chondrogenesis were induced by the human periosteal demineralized particles and dECM of cartilage origin.…”

Section: Application Of Decm-derived Bioinks In 3d Bioprintingmentioning

confidence: 99%

Recent Advances in Decellularized Matrix-Derived Materials for Bioink and 3D Bioprinting

Liu

Gong

Zhang

et al. 2023

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As an emerging 3D printing technology, 3D bioprinting has shown great potential in tissue engineering and regenerative medicine. Decellularized extracellular matrices (dECM) have recently made significant research strides and have been used to create unique tissue-specific bioink that can mimic biomimetic microenvironments. Combining dECMs with 3D bioprinting may provide a new strategy to prepare biomimetic hydrogels for bioinks and hold the potential to construct tissue analogs in vitro, similar to native tissues. Currently, the dECM has been proven to be one of the fastest growing bioactive printing materials and plays an essential role in cell-based 3D bioprinting. This review introduces the methods of preparing and identifying dECMs and the characteristic requirements of bioink for use in 3D bioprinting. The most recent advances in dECM-derived bioactive printing materials are then thoroughly reviewed by examining their application in the bioprinting of different tissues, such as bone, cartilage, muscle, the heart, the nervous system, and other tissues. Finally, the potential of bioactive printing materials generated from dECM is discussed.

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Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules

Cited by 13 publications

References 64 publications

Advances in Cartilage Tissue Engineering Using Bioinks with Decellularized Cartilage and Three-Dimensional Printing

Advances in Cartilage Tissue Engineering Using Bioinks with Decellularized Cartilage and Three-Dimensional Printing

Intraoperative Creation of Tissue-Engineered Grafts with Minimally Manipulated Cells: New Concept of Bone Tissue Engineering In Situ

Recent Advances in Decellularized Matrix-Derived Materials for Bioink and 3D Bioprinting

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