Cargo security during road transportation particularly presents a current topic in the context of transport safety. One of the key factors influencing the magnitude of impact (acceleration coefficients) during transportation is the quality of the road networks. Acceleration coefficient values directly affect the rate of inertia forces influencing the cargo. Given that the inertia force magnitudes (acceleration coefficients) are not known prior to commencing the actual transport, acceleration coefficient values known from regulations or otherwise (for example, empirically) certain established values must be used. Values of acceleration coefficients were established in EN 12195-1, a regulation typically used within the European Union. This chapter covers the approaches of this standard and provides comparison of acceleration coefficients established through regulations with those measured. Data (coefficient acceleration) from both highway transport and unpaved roads (in off-road conditions) were measured and statistically processed for comparison purposes. The transportation model presented subsequently demonstrates differences in the magnitude of inertia forces using three sets of dataacceleration coefficients obtained from the standard, from highway transport, and from off-road transport. At the same time, these secured cargoes were set into an insufficient context, where unsuitable or insufficient security of the cargo represents one of the significant risks in the occurrence of an accident.