Bovine teeth as substitute for human teeth in dental research: a review of literature

Yassen, Ghaeth H.; Platt, Jeffrey A.; Hara, Anderson T.

doi:10.2334/josnusd.53.273

Cited by 271 publications

(233 citation statements)

References 77 publications

(170 reference statements)

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“…In this study, bovine teeth were used as they have been the most widely used substitute for human teeth in dental studies and are easy to obtain in large quantities, good condition and are of a more uniform composition than that of human teeth [29]. Although bovine teeth are commonly used, some concerns about the application of data obtained from bovine to human teeth have been raised, as their chemistry and structure are not fully identical [30,31] and inconsistent data exists for microleakage of bovine dentine compared to that of human dentine [32,33].…”

Section: Discussionmentioning

confidence: 99%

Endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation

et al. 2015

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OBJECTIVES This study aims to assess the potential of a mixture of three antibiotics (TreVitaMix, TVM) as an intracanal dressing to disinfect the outer root surface by applying a new in vitro model. MATERIALS AND METHODS Fifty freshly extracted bovine roots were endodontically treated. Forty samples were then thoroughly scaled, mounted to petri dishes, gas sterilized, and randomly allocated to four groups (n = 10/group) according to their intracanal medication: sterile saline (NaCl; control, A); the TVM carrier material alone, i.e., propylene glycol (PG; B); TVM (C); and calcium hydroxide (D). In an additional group (E), the cementum was not removed and TVM was placed. Petri dishes were filled with Fastidious Anaerobe Agar, inoculated with Fusobacterium nucleatum suspension and then anaerobically incubated during 48-h intervals at 37°C up to 192 h. Inhibition zones around the roots were then measured after each incubation period (mm(2)). RESULTS Only teeth inoculated with the TVM dressing showed inhibition at all time points, whereas the other treatments showed no peri-radicular growing inhibition. Presence of cementum had no negative effect on disinfection (p = 0.9320). CONCLUSION TVM was able to penetrate through the dentine and inhibit the bacterial growth of F. nucleatum up to 192 h. CLINICAL RELEVANCE TVM might have the potential to sustainably disinfect the outer root surface in perio-endo lesions and serve as an adjunctive antimicrobial agent. Declaration of interests:The authors declare they have no conflicts of interest. AbstractObjectives To assess the potential of a mixture of three antibiotics (TreVitaMix, TVM) as an intracanal dressing to disinfect the outer root surface applying a new in vitro model. Materials and MethodsFifty freshly extracted bovine roots were endodontically treated. Forty samples were then thoroughly scaled, mounted to petri dishes, gas sterilized and randomly allocated to four groups (n=10/group) according their intracanal medication: Sterile saline NaCl (control, A), the TVM carrier material alone, i.e. propylene glycol (PG; B), TVM (C) and calcium hydroxide (D). In an additional group (E), the cementum was not removed and TVM was placed. Petri dishes were filled with Fastidiuos Anaerobe Agar, inoculated with F. nucleatum suspension and then anaerobically incubated during 48 h intervals at 37°C up to 192 h. Inhibition zones around the roots were then measured after each incubation period (mm 2 ).Results Only teeth inoculated with the TVM dressing showed inhibition at all time points, whereas the other treatments showed no peri-radicular growing inhibition. Presence of cementum had no negative effect on the disinfection (p = 0.9320).Conclusion TVM was able to penetrate through dentine and inhibit the bacterial growth of F. nucleatum up to 192 h.Clinical relevance TVM might have the potential to sustainably disinfect the outer root surface in perio-endo lesions and serve as an adjunctive antimicrobial agent.

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Section: Discussionmentioning

confidence: 99%

Endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation

et al. 2015

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“…Although there is a consensus that the use of human teeth is more relevant for conducting in vitro studies, bovine teeth were used in this study. The advantage of using bovine instead of human teeth is that the former are easy to obtain in large quantities and in good condition and have fewer enamel composition variables compared with human teeth 14,15) . Furthermore, bovine teeth have large, flat surfaces without prior caries that can influence our test results.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effects of fluoride-releasing varnish on dentin demineralization using optical coherence tomography

et al. 2014

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Effects of a fluoride-releasing varnish containing surface reaction-type prereacted glass ionomer (S-PRG) filler on dentin demineralization were examined using optical coherence tomography. Bovine incisors that were sliced and treated with undersaturated 0.1 M lactic acid buffer solution (DE group). A thin film of varnish-containing S-PRG filler was applied before demineralization (PRG group). Control was maintained in artificial saliva. Using optical coherence tomography of selected locations on the dentin surface, peak intensity (dB) and width (μm) at 1/e 2 were obtained and integrated values calculated. Although alterations in integrated values were different in each group, there was a slight but not significant increase in those for the control group and a slight but significant increase for the De group. For the PRG group, integrated values were doubled seven days after experiment initiation, followed by a significant increase. Fluoride-releasing varnish containing S-PRG fillers prevented dentin demineralization as detected by optical coherence tomography.

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“…13 After separating the roots with a low-speed diamond saw (Isomet 1000, Buehler Ltd, Lake Bluff, IL, USA), the pulps were extirpated and the pulp chamber of each tooth was filled with cotton to avoid penetration of the embedding medium. The labial surfaces of the bovine incisors were ground with wet 240-grit silicon carbide (SiC) paper to produce a flat dentin surface (Ecomet 4, Buehler Ltd).…”

Section: Surface Free Energymentioning

confidence: 99%

Influence of Light Intensity on Surface Free Energy and Dentin Bond Strength of Core Build-up Resins

Shimizu

Tsujimoto

Furuichi³

et al. 2015

Operative Dentistry

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SUMMARY Objective We examined the influence of light intensity on surface free energy characteristics and dentin bond strength of dual-cure direct core build-up resin systems. Methods Two commercially available dual-cure direct core build-up resin systems, Clearfil DC Core Automix with Clearfil Bond SE One and UniFil Core EM with Self-Etching Bond, were studied. Bovine mandibular incisors were mounted in acrylic resin and the facial dentin surfaces were wet ground on 600-grit silicon carbide paper. Adhesives were applied to dentin surfaces and cured with light intensities of 0 (no irradiation), 200, 400, and 600 mW/cm2. The surface free energy of the adhesives (five samples per group) was determined by measuring the contact angles of three test liquids placed on the cured adhesives. To determine the strength of the dentin bond, the core build-up resin pastes were condensed into the mold on the adhesive-treated dentin surfaces according to the methods described for the surface free energy measurement. The resin pastes were cured with the same light intensities as those used for the adhesives. Ten specimens per group were stored in water maintained at 37°C for 24 hours, after which they were shear tested at a crosshead speed of 1.0 mm/minute in a universal testing machine. Two-way analysis of variance (ANOVA) and a Tukey-Kramer test were performed, with the significance level set at 0.05. Results The surface free energies of the adhesive-treated dentin surfaces decreased with an increase in the light intensity of the curing unit. Two-way ANOVA revealed that the type of core build-up system and the light intensity significantly influence the bond strength, although there was no significant interaction between the two factors. The highest bond strengths were achieved when the resin pastes were cured with the strongest light intensity for all the core build-up systems. When polymerized with a light intensity of 200 mW/cm2 or less, significantly lower bond strengths were observed. Conclusions The data suggest that the dentin bond strength of core build-up systems are still affected by the light intensity of the curing unit, which is based on the surface free energy of the adhesives. On the basis of the results and limitations of the test conditions used in this study, it appears that a light intensity of >400 mW/cm2 may be required for achieving the optimal dentin bond strength.

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Bovine teeth as substitute for human teeth in dental research: a review of literature

Cited by 271 publications

References 77 publications

Endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation

Endodontic drug delivery for root surface disinfection: a laboratory feasibility evaluation

Evaluation of the effects of fluoride-releasing varnish on dentin demineralization using optical coherence tomography

Influence of Light Intensity on Surface Free Energy and Dentin Bond Strength of Core Build-up Resins

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