Here we report the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel antibiotic-containing scaffolds. Metronidazole (MET) or Ciprofloxacin/(CIP) was mixed with a polydioxanone (PDS)polymer solution at 5 and 25 wt% and processed into fibers. PDS fibers served as a control. Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), tensile testing, and high-performance liquid chromatography (HPLC) were used to assess fiber morphology, chemical structure, mechanical properties, and drug release, respectively. Antimicrobial properties were evaluated against those of Porphyromonas gingivalis/Pg and Enterococcus faecalis/Ef. Cytotoxicity was assessed in human dental pulp stem cells (hDPSCs). Statistics were performed, and significance was set at the 5% level. SEM imaging revealed a submicron fiber diameter. FTIR confirmed antibiotic incorporation. The tensile values of hydrated 25 wt% CIP scaffold were significantly lower than those of all other groups. Analysis of HPLC data confirmed gradual, sustained drug release from the scaffolds over 48 hrs. CIP-containing scaffolds significantly (p < .00001) inhibited biofilm growth of both bacteria. Conversely, MET-containing scaffolds inhibited only Pg growth. Agar diffusion confirmed the antimicrobial properties against specific bacteria for the antibiotic-containing scaffolds. Only the 25 wt% CIP-containing scaffolds were cytotoxic. Collectively, this study suggests that polymer-based antibiotic-containing electrospun scaffolds could function as a biologically safe antimicrobial drug delivery system for regenerative endodontics.
SUMMARY The present study assessed the polymerization shrinkage stress kinetics of five low-shrinkage light-cured bulk-fill resin composites: Surefil SDR flow (SF, Dentsply), Tetric EvoCeram Bulkfil (TE, Ivoclar Vivadent), Venus Bulk Fill (VB, Heraeus Kulzer), x-tra fil (XF, Voco), and experimental bulk fill (FB, 3M ESPE). Filtek Z250 (FZ, 3M ESPE) was used as a control. Real-time shrinkage stress of investigated composites was measured using a tensometer; maximum shrinkage stress, stress rate (Rmax), and time to reach maximum stress rate (tmax) were recorded. Flexural strength and modulus were measured using a standard procedure, and curing efficiency of 4-mm long specimens was determined using bottom/top percentage Knoop microhardness. Data were analyzed using one-way analysis of variance and Bonferroni multiple range tests at a significance level of α=0.05. Results of shrinkage stress, Rmax, and tmax of all bulk-fill materials were significantly lower (p<0.05) than those of the control except for XF. All tested bulk-fill materials were able to achieve acceptable curing efficiency (≥80% bottom/top percentage) at 4-mm depth. In conclusion, this study reports a significant reduction in polymerization shrinkage stress while maintaining comparable curing efficiency at 4 mm for some bulk-fill composites and supports their potential use in posterior clinical situations.
The aim of this paper was to review in vitro and in situ studies that directly compared the use of bovine teeth as a substitute for human teeth in dental experiments. A PubMed search was conducted for papers published from 1953 to December 30, 2010 using the following keywords: "human bovine enamel" or "human bovine dentin" or "human bovine teeth". The abstracts of the studies resulting from the keyword search were read, and all papers that compared human and bovine teeth were fully read. Only original articles written in English and directly comparing human and bovine substrates were included in the review. The search was supplemented by manual searches of the reference lists from each identified paper. Out of 76 studies initially selected, 68 fulfilled the selection criteria for inclusion. The studies covered seven categories: dental morphology, chemical composition, physical properties, dental caries, dental erosion/abrasion, bonding/adhesive strength, and marginal microleakage. Inconsistent data exist regarding whether bovine teeth can be considered an appropriate substitute for human teeth in dental research. Morphological, chemical composition and physical property differences between the two substrates must be considered when interpreting results obtained from any experiment using bovine tooth substrate. (J Oral Sci 53, 273-282, 2011)
Fracture resistance and mode of failure of CAD/CAM-fabricated monoblock endocrowns varies widely between materials. Clinicians should be cautious with material selection for endocrown restorations. SUMMARYThis study assessed marginal leakage and fracture resistance of computer-aided design/ computer-aided manufacturing (CAD/CAM) fabricated ceramic crowns with intracoronal extensions into the pulp chambers of endodontically treated teeth (endocrowns) using either feldspathic porcelain (CEREC Blocks [CB], Sirona Dental Systems GmbH, Bensheim, Germany), lithium disilicate (e.max [EX], Ivoclar Vivadent, Schaan, Liechtenstein), or resin nanoceramic (Lava Ultimate [LU], 3M ESPE, St Paul, MN, USA).). Thirty extracted human permanent maxillary molars were endodontically treated. Standardized preparations were done with 2-mm intracoronal extensions of the endocrowns into the pulp chamber. Teeth were divided into three groups (n=10); each group was restored with standardized CAD/CAM fabricated endocrowns using one of the three tested materials. After cementation with resin cement, specimens were stored in distilled water at 378C for one week, subjected to thermocycling, and immersed in a 5% methylene-blue dye solution for 24 hours. A compressive load was applied at 35 degrees to long axis of the teeth using a universal testing machine until failure. Failure load was recorded, and specimens were examined under a stereomicroscope for modes of failure and microleakage. Results were analyzed using one-way analysis of variance and Bonferroni post hoc multiple comparison tests (a=0.05). LU showed significantly (p,0.05) higher fracture resistance and more favorable fracture mode (ie, fracture of the endocrown without fracture of tooth) as well as higher dye penetration than CB and EX. In conclusion, although using resin
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