Bovine serum albumin nanoparticles improve the antitumour activity of curcumin in a murine melanoma model

Camargo, Luciana Erzinger Alves de; Ludwig, Daniel Brustolin; Tominaga, Tânia Toyomi; Lipinski, Leandro Cavalcante; Fávero, Giovani Marino; Mainardes, Rubiana Mara; Khalil, Najeh Maissar

doi:10.1080/02652048.2018.1526340

Cited by 17 publications

(4 citation statements)

References 37 publications

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“…Therefore, it will be important in future studies to assess the effectiveness of oral or intraperitoneal administration of AF4 to tumor-bearing mice. Delivery of an optimal concentration of AF4 to the tumor microenvironment via the oral or intraperitoneal route will likely require a nanoparticle delivery vehicle of the type that has been reported to greatly enhance the bioavailability of other bioactive phytochemicals such as curcumin [34,35]. In this regard, nanoparticles have also been employed to deliver plant extracts with anticancer properties to various types of cancer cells [36].…”

Section: Discussionmentioning

confidence: 99%

Apple Peel Flavonoid Fraction 4 Suppresses Breast Cancer Cell Growth by Cytostatic and Cytotoxic Mechanisms

2019

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Many dietary flavonoids possess anti-cancer activities. Here, the effect of apple peel flavonoid fraction 4 (AF4) on the growth of triple-negative (MDA-MB-231, MDA-MB-468), estrogen receptor-positive (MCF-7), and HER2-positive (SKBR3) breast cancer cells was determined and compared with the effect of AF4 on normal mammary epithelial cells and dermal fibroblasts. AF4 inhibited breast cancer cell growth in monolayer cultures, as well as the growth of MCF-7 spheroids, without substantially affecting the viability of non-malignant cells. A sub-cytotoxic concentration of AF4 suppressed the proliferation of MDA-MB-231 cells by inhibiting passage through the G0/G1 phase of the cell cycle. AF4-treated MDA-MB-231 cells also exhibited reduced in vitro migration and invasion, and decreased Akt (protein kinase B) signaling. Higher concentrations of AF4 were selectively cytotoxic for MDA-MB-231 cells. AF4 cytotoxicity was associated with the intracellular accumulation of reactive oxygen species. Importantly, intratumoral administration of AF4 suppressed the growth of MDA-MB-231 xenografts in non-obese diabetic severe combined immunodeficient (NOD-SCID) female mice. The selective cytotoxicity of AF4 for breast cancer cells, combined with the capacity of sub-cytotoxic AF4 to inhibit breast cancer cell proliferation, migration, and invasion suggests that flavonoid-rich AF4 (and its constituents) has potential as a natural therapeutic agent for breast cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Apple Peel Flavonoid Fraction 4 Suppresses Breast Cancer Cell Growth by Cytostatic and Cytotoxic Mechanisms

2019

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“… 16 BSA is commonly used in studies due to its low price and easy purification process. 17 HSA is frequently used to avoid losses from animal serum albumin, one of which is bovine spongiform encephalopathy. 18 Furthermore, the ability of OVA to create foam and gel networks has led to its widespread use in the food industry.…”

Section: Methodsmentioning

confidence: 99%

Factors Affecting the Synthesis of Bovine Serum Albumin Nanoparticles Using the Desolvation Method

Tanjung,

Dewi,

Gatera

et al. 2024

NSA

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Currently, protein-based nanoparticles are in high demand as drug delivery systems due to their exceptional qualities, including nontoxicity, nonantigenicity, and biodegradability. Other qualities include high nutritional value, abundance of renewable resources, excellent drug binding capacity, greater stability during storage and in vivo, as well as ease of upgrading during manufacture. Examples of protein suitable for this purpose include ovalbumin (OVA) derived from egg white, human serum albumin (HSA), and bovine serum albumin (BSA). To create albumin nanoparticles, six different processes have been investigated in depth and are frequently used in drug delivery systems. These included desolvation, thermal gelation, emulsification, NAB technology, self-assembly, and nanospray drying. Several experimental conditions in the synthesis of albumin nanoparticles can affect the physicochemical characterization. Therefore, this study aimed to provide an overview of various experimental conditions capable of affecting the physicochemical characteristics of BSA nanoparticles formed using the desolvation method. By considering the variation in optimal experimental conditions, a delivery system of BSA nanoparticles with the best physicochemical characterization results could be developed.

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“…There are numerous studies that reveal the effects of added protein on the properties of CCM through complexation with milk proteins, for example, casein [ 103 ], whey protein isolate (WPI) [ 104 ], bovine serum albumin [ 105 ], or with plant-derived proteins such as sunflower protein isolate [ 106 ], mung bean protein [ 107 ], and soy protein [ 108 , 109 ].…”

Section: Curcumin Bioavailability Improvingmentioning

confidence: 99%

Strategies for Improving Bioavailability, Bioactivity, and Physical-Chemical Behavior of Curcumin

et al. 2022

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Curcumin (CCM) is one of the most frequently explored plant compounds with various biological actions such as antibacterial, antiviral, antifungal, antineoplastic, and antioxidant/anti-inflammatory properties. The laboratory data and clinical trials have demonstrated that the bioavailability and bioactivity of curcumin are influenced by the feature of the curcumin molecular complex types. Curcumin has a high capacity to form molecular complexes with proteins (such as whey proteins, bovine serum albumin, β-lactoglobulin), carbohydrates, lipids, and natural compounds (e.g., resveratrol, piperine, quercetin). These complexes increase the bioactivity and bioavailability of curcumin. The current review provides these derivatization strategies for curcumin in terms of biological and physico-chemical aspects with a strong focus on different type of proteins, characterization methods, and thermodynamic features of protein–curcumin complexes, and with the aim of evaluating the best performances. The current literature review offers, taking into consideration various biological effects of the CCM, a whole approach for CCM-biomolecules interactions such as CCM-proteins, CCM-nanomaterials, and CCM-natural compounds regarding molecular strategies to improve the bioactivity as well as the bioavailability of curcumin in biological systems.

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Bovine serum albumin nanoparticles improve the antitumour activity of curcumin in a murine melanoma model

Cited by 17 publications

References 37 publications

Apple Peel Flavonoid Fraction 4 Suppresses Breast Cancer Cell Growth by Cytostatic and Cytotoxic Mechanisms

Apple Peel Flavonoid Fraction 4 Suppresses Breast Cancer Cell Growth by Cytostatic and Cytotoxic Mechanisms

Factors Affecting the Synthesis of Bovine Serum Albumin Nanoparticles Using the Desolvation Method

Strategies for Improving Bioavailability, Bioactivity, and Physical-Chemical Behavior of Curcumin

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