Factors Affecting the Synthesis of Bovine Serum Albumin Nanoparticles Using the Desolvation Method

Abstract

Currently, protein-based nanoparticles are in high demand as drug delivery systems due to their exceptional qualities, including nontoxicity, nonantigenicity, and biodegradability. Other qualities include high nutritional value, abundance of renewable resources, excellent drug binding capacity, greater stability during storage and in vivo, as well as ease of upgrading during manufacture. Examples of protein suitable for this purpose include ovalbumin (OVA) derived from egg white, human serum albumin (HSA), and bovine serum albumin (BSA). To create albumin nanoparticles, six different processes have been investigated in depth and are frequently used in drug delivery systems. These included desolvation, thermal gelation, emulsification, NAB technology, self-assembly, and nanospray drying. Several experimental conditions in the synthesis of albumin nanoparticles can affect the physicochemical characterization. Therefore, this study aimed to provide an overview of various experimental conditions capable of affecting the physicochemical characteristics of BSA nanoparticles formed using the desolvation method. By considering the variation in optimal experimental conditions, a delivery system of BSA nanoparticles with the best physicochemical characterization results could be developed.

Crafting Docetaxel-Loaded Albumin Nanoparticles Through a Novel Thermal-Driven Self-Assembly/Microfluidic Combination Technology: Formulation, Process Optimization, Stability, and Bioavailability

“…Addressing batch-to-batch variability in the industrial production of BSA NPs is a complex challenge that encompasses various factors, from the physicochemical characteristics of the nanoparticles to the synthesis and processing parameters. 66,67 In this study, both BSA NPs and freeze-dried powders exhibit good reproducibility. The formulation composition and manufacturing process of DTX-BSA-NPs were successfully optimized by the thermaldriven self-assembly/microfluidic combination technology, providing an experimental foundation for future industrialscale production.…”