Bone healing in critical-size defects treated with platelet-rich plasma: a histologic and histometric study in the calvaria of diabetic rat

Mariano, Ronaldo Célio; Messora, Michel Reis; Morais, Adriana de; Nagata, Maria José Hitomi; Furlaneto, Flávia Aparecida Chaves; Avelino, Cassia Carneiro; Paula, Fernanda Borges de Araújo; Ferreira, Sueli Mara Soares Pinto; Pinheiro, Marcelo de Medeiros; Sene, João Paulo de

doi:10.1016/j.tripleo.2009.08.003

Cited by 53 publications

(33 citation statements)

References 35 publications

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“…On the other hand, Fontana et al (35) examined osteogenesis around titanium implants placed at rat tibia with or without PRP and reported significantly more new bone formation at sites treated with PRP after four weeks. Mariano et al (36) histologically analyzed the influence of PRP on bone healing in surgically created critical-size defects in the calvaria of diabetic rats at the end of a month and found that PRP significantly enhanced bone healing both qualitatively and quantitatively. The conflictive results of the different studies about treatment with PRP alone may be due to different defect models, as well as different evaluation techniques and periods.…”

Section: Discussionmentioning

confidence: 99%

Double-application of platelet-rich plasma on bone healing in rabbits

Özdemir¹,

Kurtiş²,

Tüter³

et al. 2012

Med Oral

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Objective: Platelet-rich plasma (PRP) is considered to enhance bone formation especially at early stages of wound healing, depending on the limited and short life-span of platelets and growth factors. The aim of this study was to evaluate efficacy of double-application of PRP (DA-PRP) on bone healing in a rabbit calvarial defect model. Study design: Twenty-eight rabbits, each had two surgically prepared calvarial bone defects (10mm diameter), were included in this study and randomly divided into six groups. Defects (n=56) were treated with single-application of PRP (SA-PRP)(n=10), SA-PRP and beta-tricalciumphosphate (SA-PRP+TCP)(n=10), DA-PRP (n=8), DA-PRP and beta-tricalciumphosphate (DA-PRP+TCP)(n=8), beta-tricalciumphosphate (TCP)(n=10) or left empty (Control)(n=10). Animals were sacrificed at 30 days postoperatively. Results: The new bone (NB%) and defect fill (DF%) percentages were calculated from histological slides by image-analyzer software and statistically analysed. All test groups showed higher NB% than control, but differences among all groups were insignificant. The TCP treated groups had significantly higher DF% than groups treated without TCP, however the DF% differences between control, SA-PRP and DA-PRP or TCP, SA-PRP+TCP or DA-PRP+TCP were insignificant. Conclusion: Although new bone formation was histomorphologically remarkable at double-application PRP groups, statistical analyses of the histomorphometric data revealed no significant difference. Key words: Platelet-Rich Plasma, double application, bone formation, wound healing.

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Section: Discussionmentioning

confidence: 99%

Double-application of platelet-rich plasma on bone healing in rabbits

Özdemir¹,

Kurtiş²,

Tüter³

et al. 2012

Med Oral

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“…However, these effects were dependent on the PRP composition [10]. Somehow data become controversial when in vivo settings are compared, since opposite outcomes are equally available in the scientific literature in favor [11][12][13][14][15][16][17][18][19][20][21] or against [19,[22][23][24][25][26][27] the addition of PRP to biologic and synthetic graft materials for bone regeneration purposes. Interestingly, the association of PRP with mesenchymal stem cells showed mostly satisfying results [28,29] in the field.…”

Section: Introductionmentioning

confidence: 99%

Cytokine, chemokine, and growth factor profile of platelet-rich plasma

et al. 2016

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During wound healing, biologically active molecules are released from platelets. The rationale of using platelet-rich plasma (PRP) relies on the concentration of bioactive molecules and subsequent delivery to healing sites. These bioactive molecules have been seldom simultaneously quantified within the same PRP preparation. In the present study, the flexible Bio-Plex system was employed to assess the concentration of a large range of cytokines, chemokines, and growth factors in 16 healthy volunteers so as to determine whether significant baseline differences may be found. Besides IL-1b, IL-1ra, IL-4, IL-6, IL-8, IL-12, IL-13, IL-17, INF-γ, TNF-α, MCP-1, MIP-1a, RANTES, bFGF, PDGF, and VEGF that were already quantified elsewhere, the authors reported also on the presence of IL-2, IL-5, IL-7, IL-9, IL-10, IL-15 G-CSF, GM-CSF, Eotaxin, CXCL10 chemokine (IP-10), and MIP 1b. Among the most interesting results, it is convenient to mention the high concentrations of the HIV-suppressive and inflammatory cytokine RANTES and a statistically significant difference between males and females in the content of PDGF-BB. These data are consistent with previous reports pointing out that gender, diet, and test system affect the results of platelet function in healthy subjects, but seem contradictory when compared to other quantification assays in serum and plasma. The inconsistencies affecting the experimental results found in literature, along with the variability found in the content of bioactive molecules, urge further research, hopefully in form of randomized controlled clinical trials, in order to find definitive evidence of the efficacy of PRP treatment in various pathologic and regenerative conditions.

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“…Consequently, this promotes a failure in the expression of genes that regulate osteoblast differentiation, thereby reducing bone formation. 3,6 Therefore, HDDM could be considered an ideal graft material since it stimulates osteoblast differentiation and proliferation and potentially leads to efficient bone regeneration. To clarify the osteogenic activity of the DDM, previous studies have demonstrated it to be a bioactive tissue able to stimulate bone regeneration, inducing osteoblastic differentiation and proliferation and chemotaxis.…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3][4][5] To obtain effective bone regeneration or osseointegration the following are necessary: osteoprogenitor and osteoconductive cells, which offer potential to differentiate and facilitate the various stages of bone regeneration, growth factors, structural integrity with the absence of any kind of local infection, and, in long bones, mechanical immobilization. [6][7][8] Many strategies have been used to accelerate bone repair or to act as an alternative bioactive material scaffold, including demineralized dentin matrix (DDM) 1,9 and platelet-rich plasma (PRP).…”

mentioning

confidence: 99%

“…[6][7][8] Many strategies have been used to accelerate bone repair or to act as an alternative bioactive material scaffold, including demineralized dentin matrix (DDM) 1,9 and platelet-rich plasma (PRP). 2,3,[8][9][10][11][12] PRP contains several proteins that are of great benefit in wound healing. These proteins regulate cell migration and proliferation (mitogenesis), remodel the extracellular matrix, and promote angiogenesis, creating a favourable environment that quickly stimulates tissue repair and wound healing.…”

mentioning

confidence: 99%

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