Double-application of platelet-rich plasma on bone healing in rabbits

Abstract

Objective: Platelet-rich plasma (PRP) is considered to enhance bone formation especially at early stages of wound healing, depending on the limited and short life-span of platelets and growth factors. The aim of this study was to evaluate efficacy of double-application of PRP (DA-PRP) on bone healing in a rabbit calvarial defect model. Study design: Twenty-eight rabbits, each had two surgically prepared calvarial bone defects (10mm diameter), were included in this study and randomly divided into six groups. Defects (n=56) were treated with single-application of PRP (SA-PRP)(n=10), SA-PRP and beta-tricalciumphosphate (SA-PRP+TCP)(n=10), DA-PRP (n=8), DA-PRP and beta-tricalciumphosphate (DA-PRP+TCP)(n=8), beta-tricalciumphosphate (TCP)(n=10) or left empty (Control)(n=10). Animals were sacrificed at 30 days postoperatively. Results: The new bone (NB%) and defect fill (DF%) percentages were calculated from histological slides by image-analyzer software and statistically analysed. All test groups showed higher NB% than control, but differences among all groups were insignificant. The TCP treated groups had significantly higher DF% than groups treated without TCP, however the DF% differences between control, SA-PRP and DA-PRP or TCP, SA-PRP+TCP or DA-PRP+TCP were insignificant. Conclusion: Although new bone formation was histomorphologically remarkable at double-application PRP groups, statistical analyses of the histomorphometric data revealed no significant difference. Key words: Platelet-Rich Plasma, double application, bone formation, wound healing.

The Comparison Of The Effects Of Platelet Rich Plasma And Demineralized Bone Matrix On Critical Bone Defects: An Experimental Study On Rats

“…Upper yellow layer consisted of platelet-rich and platelet-poor plasma, while lower red layer consisted of erythrocytes and leukocytes, as has previously been documented. [30] Lower red layer has been reported to be rich in platelets that recently entered the circulation. [31,32] Total plasma consisting of complete upper yellow layer and top 1 to 2 mm of lower red layer was transferred to sterile monovette.…”

“…After second centrifugation at 4000 rpm for 7 minutes, approximately 0.7 mm at the bottom of the monovette was platelet-rich plasma and upper portion was mostly platelet-poor plasma. [30] Supernatant platelet-poor plasma was collected and removed by pipette. Remaining platelet-rich plasma was then carefully transferred into application injector.…”

Cranial vault reconstruction with bone morphogenetic protein, calcium phosphate, acellular dermal matrix, and calcium alginate in mice

“…It is commercially available as blocks or granules. There are several papers combining TCP to GF, with positive and negative results 40,44 . Afifi et al 45 have demonstrated that TCP alone has a non-negligible tax of complications in cranioplasties, especially when inserted in previously irradiated areas, or for those in contact with paranasal sinuses.…”

Osteocalcin and Osteonectin Expression After Double-Application of Platelet-Rich Plasma in Rabbits

“…Most of the experimental ( 7 , 8 , 10 ) and clinical ( 1 , 5 , 6 ) studies which examine the effects of PRP on the healing of bony defects, have focused on the single-application of PRP to the wound area. Recently, promising results on soft tissue healing have been suggested by multiple-application of PRP ( 11 , 12 , 13 , 14 , 15 ). Crovetti et al ( 11 ) reported that following topical treatment with multi-application of PRP, 9 out of 24 cutaneous chronic nonresponsive severe ulcers healed completely and eight healed by more than 50%.…”