Homogenous demineralized dentin matrix and platelet-rich plasma for bone tissue engineering in cranioplasty of diabetic rabbits: biochemical, radiographic, and histological analysis

Gomes, Mônica Fernandes; Valva, Vivian Neves; Vieira, Evanice Menezes Marçal; Giannasi, Lílian Chrystiane; Salgado, Miguel Angel Castillo; Vilela-Goulart, M. G.

doi:10.1016/j.ijom.2015.09.009

Cited by 22 publications

(33 citation statements)

References 34 publications

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“…Many in vitro animal and clinical studies have been conducted since then and have confirmed the biocompatibility, biodegradability and osteoinductive and osteoconductive potency of the dentin matrix [15][16][17]. For example, Gomes and couleges18 have conducted histological evaluations on the osteoinductivity of DDM with calvarial defects in rabbits and have verified that DDM have chemotactic properties for osteoprogenitor cells and osteoblasts, which may promote local bone regeneration. Murata and couleges[19][20][21] reported the clinical usage of autogenous DDM from wisdom tooth grafted back into the socket immediately after extraction.…”

mentioning

confidence: 99%

Autogenous DDM versus Bio‐Oss granules in GBR for immediate implantation in periodontal postextraction sites: A prospective clinical study

Zhu

Huang

2018

Clin Implant Dent Rel Res

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mentioning

confidence: 99%

Autogenous DDM versus Bio‐Oss granules in GBR for immediate implantation in periodontal postextraction sites: A prospective clinical study

Zhu

Huang

2018

Clin Implant Dent Rel Res

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“…High tissue oxidative stress alters endothelial cell functions and elevates activity of matrix metalloproteinases, inducing the production of free radicals, increased vascular dysfunction, and cellular death. 6,18,19,20 These molecular mechanisms could justify the severity of periodontal disease, found mainly in the D and 160 J-LD groups.…”

Section: Discussionmentioning

confidence: 98%

“…1,2 Hyperglycaemia over a long period results in various classic complications, including periodontopathy. 1,3,4,5,6 Several studies have demonstrated significant alterations to bone and mineral metabolism, which are related to the following factors: decrease of osteoclasts and osteoblasts, enhance of apoptosis of osteoblasts, and reduces osteocalcin synthesis. These processes lead to extensive bone loss and, as a consequence, impaired bone turnover and formation.…”

Section: Introductionmentioning

confidence: 99%

“…These processes lead to extensive bone loss and, as a consequence, impaired bone turnover and formation. 3,6,7,8 Alternative therapies have been proposed to stimulate alveolar bone remodeling and to favor a better response in gingival and periodontal ligament tissues, especially in the supported treatments for orthodontics. In particular, the photobiomodulation (PBM), previously known as low-level laser therapy (LLLT), has showed great effectiveness due to its analgesic, anti-inflammatory and photobiostimulatory properties, resulting in a faster tooth movement with no periodontal tissue side effects.…”

Section: Introductionmentioning

confidence: 99%

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Effects of the photobiomodulation using different energy densities on the periodontal tissues under orthodontic force in rats with type 2 diabetes mellitus

Gomes¹,

Goulart²,

Giannasi³

et al. 2018

Braz. oral res.

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To evaluate the impact of the GaAlAs diode laser with energy densities of 160 J/cm 2 , 320 J/cm 2 , and 640 J/cm 2 on the periodontal tissues under continuous orthodontic force application and on the rate of orthodontic tooth movement in rats with type-2 diabetes mellitus. The intensity of primary alveolar bone formation was also investigated through the immune-positive osteocytes for OPN antibody. Forty adult male Wistar rats were divided into eight groups of 5 rats: normoglycemic (N), 160 J-laser-normoglycemic (160 J-LN), 320 J-laser-normoglycemic (320 J-LN), 640 J-laser-normoglycemic (640 J-LN), diabetic (D), 160 J-laser-diabetic (160 J-LD), 320 J-laser-diabetic (320 J-LD), and 640 J-laser-diabetic (640 J-LD) rats. Diabetes mellitus was induced by a single intravenous injection of 40 mg/kg monohydrated-alloxan. An orthodontic force magnitude of 20cN was applied. The laser parameters were continuous emission of 780-nm wavelength, output power of 20mW, and fiber probe with a spot size of 0.04 cm in diameter. Radiographic, histomorphological, and immunohistochemical analysis were performed after a period of 21 days. The photobiomodulation using the energy density of 640 J/cm 2 strongly stimulated the alveolar bone formation and contributed the reorganization of the soft periodontal tissues, followed by the 320 J/cm 2 . Extensive alveolar bone loss, intense infiltration of inflammatory cells, and degradation of the PDJ tissue were mainly found in the D and 160 J-LD groups. The rate of orthodontic tooth movement was represented by the interdental distance between the cementoenamel junctions of the right mandibular first and second molars. This distance was larger in the diabetic groups (D: 39.98±1.97, 160 J-LD: 34.84±6.01, 320 J-LD: 29.82±1.73, and 640 J-LD: 35.47±4.56) than in the normoglycemic groups (N: 21.13±1.19; 160 J-LN: 22.69±0.72, 320 J-LN: 22.28±0.78, and 640 J-LN: 24.56±2.11). The number of osteopontin-positive osteocytes was significantly greater in the 640 J-LD (14.72 ± 0.82; p < 0.01) and 640 J-LN (13.62 ± 1.33; p < 0.05) groups than with D (9.82 ± 1.17) and 160 J-LD (9.77 ± 1.10) groups. Therefore, the energy density of 640 J/cm 2 provided the best maintenance and integrity of the periodontal tissue microarchitecture under continuous orthodontic force when compared with the other dosages, mainly in the uncontrolled diabetic rats. The interdental distance was greater in the D and 160 J-LD groups due to presence of severe periodontitis caused by diabetes plus the mechanical stress generated by continuous orthodontic forces, implying, thus, an insufficient biostimulatory effect for the dosage of 160 J/cm 2 .

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“…Their histological analysis revealed that HDDM promoted not only increased bone formation but also superior bony microstructure owing to graft's osteoinductive and osteoconductive activities, while PRP stimulated angiogenesis and red bone marrow formation. [20] In our experience, the use of “sticky bone” preparation was particularly useful for alveolar ridge augmentation as bone graft trapped within cross-linked fibrin meshwork prevents any undesired movement of graft particles during healing phase. This allows stabilization of the bone graft onto the defect without having to use any bone tacks or titanium mesh, thereby accelerating tissue healing.…”

Section: Discussionmentioning

confidence: 99%

Ridge augmentation using sticky bone: A combination of human tooth allograft and autologous fibrin glue

Joshi

D'Lima

Karde

et al. 2019

J Indian Soc Periodontol

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Homogenous demineralized dentin matrix and platelet-rich plasma for bone tissue engineering in cranioplasty of diabetic rabbits: biochemical, radiographic, and histological analysis

Cited by 22 publications

References 34 publications

Autogenous DDM versus Bio‐Oss granules in GBR for immediate implantation in periodontal postextraction sites: A prospective clinical study

Autogenous DDM versus Bio‐Oss granules in GBR for immediate implantation in periodontal postextraction sites: A prospective clinical study

Effects of the photobiomodulation using different energy densities on the periodontal tissues under orthodontic force in rats with type 2 diabetes mellitus

Ridge augmentation using sticky bone: A combination of human tooth allograft and autologous fibrin glue

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