Bone - Graft Delivery Systems of Type PLGA- gentamicin and Collagen - hydroxyapatite - gentamicine

Ionescu, O. L.; Ciocîlteu, Maria Viorica; Manda, Costel Valentin; Neacșu, Ionela Andreea; Ficai, Anton; Amzoiu, Emilia; Stiolica, Adina Turcu; Croitoru, Octavian; Neamțu, Johny

doi:10.37358/mp.19.3.5224

Cited by 10 publications

(6 citation statements)

References 11 publications

(11 reference statements)

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“…Bone defects require rehabilitation, mainly to avoid severe alveolar bone resorption that compromises bone quantity, morphology, and quality, which prevents the failure of dental implant placement, the maintaining of the normal anatomic outline, the elimination of empty space, aesthetic restoration, as well as drugs encapsulation and delivery [3][4][5]. Until now, only the autografts and, partially, allografts could fulfil the desired properties such as biocompatibility, osteoconductivity, and osteoinductivity for a better bone substitution ability, albeit entailing important risks [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Nano-Hydroxyapatite vs. Xenografts: Synthesis, Characterization, and In Vitro Behavior

et al. 2021

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This research focused on the synthesis of apatite, starting from a natural biogenic calcium source (egg-shells) and its chemical and morpho-structural characterization in comparison with two commercial xenografts used as a bone substitute in dentistry. The synthesis route for the hydroxyapatite powder was the microwave-assisted hydrothermal technique, starting from annealed egg-shells as the precursor for lime and di-base ammonium phosphate as the phosphate precursor. The powders were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray analysis (EDAX), transmission electron microscopy (TEM), X-ray fluorescence spectroscopy (XRF), and cytotoxicity assay in contact with amniotic fluid stem cell (AFSC) cultures. Compositional and structural similarities or differences between the powder synthesized from egg-shells (HA1) and the two commercial xenograft powders—Bio-Oss®, totally deproteinized cortical bovine bone, and Gen-Os®, partially deproteinized porcine bone—were revealed. The HA1 specimen presented a single mineral phase as polycrystalline apatite with a high crystallinity (Xc 0.92), a crystallite size of 43.73 nm, preferential growth under the c axes (002) direction, where it mineralizes in bone, a nano-rod particle morphology, and average lengths up to 77.29 nm and diameters up to 21.74 nm. The surface of the HA1 nanoparticles and internal mesopores (mean size of 3.3 ± 1.6 nm), acquired from high-pressure hydrothermal maturation, along with the precursor’s nature, could be responsible for the improved biocompatibility, biomolecule adhesion, and osteoconductive abilities in bone substitute applications. The cytotoxicity assay showed a better AFSC cell viability for HA1 powder than the commercial xenografts did, similar oxidative stress to the control sample, and improved results compared with Gen-Os. The presented preliminary biocompatibility results are promising for bone tissue regeneration applications of HA1, and the study will continue with further tests on osteoblast differentiation and mineralization.

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Section: Introductionmentioning

confidence: 99%

Nano-Hydroxyapatite vs. Xenografts: Synthesis, Characterization, and In Vitro Behavior

et al. 2021

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“…Such systems aim at the formation of the material providing the stable release of antibiotics while supporting bone-tissue growth [ 148 , 149 ]. For example, Ionescu et al [ 150 ] performed the synthesis of biocomposite materials consisting of hydroxyapatite, gentamycin, and collagen. In the first step, a polymer matrix with hydroxyapatite was obtained.…”

Section: Conjugates For Drug Deliverymentioning

confidence: 99%

Review of the Applications of Biomedical Compositions Containing Hydroxyapatite and Collagen Modified by Bioactive Components

et al. 2021

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Regenerative medicine is becoming a rapidly evolving technique in today’s biomedical progress scenario. Scientists around the world suggest the use of naturally synthesized biomaterials to repair and heal damaged cells. Hydroxyapatite (HAp) has the potential to replace drugs in biomedical engineering and regenerative drugs. HAp is easily biodegradable, biocompatible, and correlated with macromolecules, which facilitates their incorporation into inorganic materials. This review article provides extensive knowledge on HAp and collagen-containing compositions modified with drugs, bioactive components, metals, and selected nanoparticles. Such compositions consisting of HAp and collagen modified with various additives are used in a variety of biomedical applications such as bone tissue engineering, vascular transplantation, cartilage, and other implantable biomedical devices.

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“…The method used for evaluating the encapsulation efficiency was detailed in a previous study [24,25]. Briefly, 5 mg gentamicin loaded PLGA nanoparticles were suspended in 1 mL borate buffer, pH = 10.…”

Section: Encapsulation Efficiencymentioning

confidence: 99%

Process Optimization Using Quality by Design (Qbd) Approach of a Gentamicin Loaded Plga Biocomposite

et al. 2021

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Osteomyelitis continues to be a major concern when orthopedic surgery is performed. Orthopedic infections have an incidence of 5% to 10% but their management proves to be quite difficult due to both biofilm formation and limited access of the drug to the infected area when systemic treatment is employed. The aim of the study was to optimize the synthesis process of a gentamicin loaded poly(-lactic-co-glycolic) acid (PLGA) based biodegradable composite by varying parameters that affect both efficiency encapsulation and nanoparticle size. Furthermore, a kinetic study was conducted to study the biodegradation process of the polymer. Gentamicin loaded PLGA nanoparticles were obtained using the double emulsion technique which allows the variation of several factors such as gentamycin concentration, PLGA concentration, buffer concentration and stirring speed. Out of the four factors evaluated, gentamicin concentration had the highest impact on both encapsulation efficiency and nanoparticle size. A few relevant interactions between factors were also registered.

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Bone - Graft Delivery Systems of Type PLGA- gentamicin and Collagen - hydroxyapatite - gentamicine

Cited by 10 publications

References 11 publications

Nano-Hydroxyapatite vs. Xenografts: Synthesis, Characterization, and In Vitro Behavior

Nano-Hydroxyapatite vs. Xenografts: Synthesis, Characterization, and In Vitro Behavior

Review of the Applications of Biomedical Compositions Containing Hydroxyapatite and Collagen Modified by Bioactive Components

Process Optimization Using Quality by Design (Qbd) Approach of a Gentamicin Loaded Plga Biocomposite

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