Bone Augmentation of Peri-Implant Dehiscence Defects Using Multilaminated Small Intestinal Submucosa as a Barrier Membrane: An Experimental Study in Dogs

Abstract

Objective. The aim of the study is to evaluate the effects of multilaminated small intestinal submucosa (mSIS) combined with bone substitute material to repair peri-implant defects during guided bone regeneration procedures. Methods. Twelve implants were placed in bilateral lower premolars of three beagle dogs, and a peri-implant buccal bone defect (3 mm width and 4 mm height) was created at each implant site. A total of 12 sites were filled with a particulate bone substitute material and then randomly divided into three treatment groups: covered by mSIS membrane (mSIS group), covered by collagen membrane (BG group), and no treatment (control group), each group of four sites. After 12 weeks of healing, all of the animals were euthanized and dissected blocks were obtained for micro-computed tomography (micro-CT) and histological analyses. Results. Micro-CT results revealed similar horizontal width of augmented tissue and new bone formation between mSIS and BG groups (P<0.05). Histological analyses revealed that the differences in horizontal widths of newly formed bone and bone-to-implant contact between mSIS and BG groups were not significant (P>0.05). All of these parameters were significantly different from those in the control group (P<0.05). Conclusions. These findings confirmed that mSIS combined with the bone substitute material enhanced bone regeneration in peri-implant defects, in a manner similar to that of a collagen membrane.

The Effect of Different Types of Collagen Membranes on Peri-Implant Dehiscence Defects

“…All parameters differed significantly from the control group, which was left empty. This study confirmed that the mSIS membrane applied with a bone graft could produce bone regeneration similar to collagen membranes 44 . The study design and comparison of the effect of membranes on BIC are similar to our study.…”

“…This study confirmed that the mSIS membrane applied with a bone graft could produce bone regeneration similar to collagen membranes. 44 The study design and comparison of the effect of membranes on BIC are similar to our study. However, in our study, the effects of collagen membranes on BIC between each other were compared.…”

The application of small intestinal submucosa in tissue regeneration

“…A common approach to enhance the mechanical properties of SIS membranes is to increase the number of layers of the SIS membrane. In a study of SIS membranes as guide bone regeneration (GBR) membranes for peri-implant dehiscence defects in beagle dogs, the 8-layer cell-free SIS membrane not only had superior mechanical properties over collagen membranes when wet, but also exhibited collagen membrane-like ways to enhance bone regeneration of peri-implant defects [ 90 ]. In another study, 2- and 4-layer SIS membranes were used as tissue-engineered heart valves for valve replacement in juvenile non-human primates.…”

Comparison of the efficacy of different biodegradable membranes in guided bone/tissue regeneration: a systematic review and network meta-analysis

“…(2) NCCM: Twenty-two studies described NCCM complications. Nineteen studies reported that the animals healed well after surgery, with no significant inflammatory response observed and no signs of infection, wound dehiscence, or membrane exposure [32,34,42,43,46,65,67,73,79,80,86,88,92,95,96,99,100,103]. Two studies reported a chronic inflammatory response [34,60].…”