Blood clearance of Howell–Jolly bodies in an experimental autogenic splenic implant model

Abstract

Splenectomy has been practised routinely, both in the emergency setting and as a therapeutic elective procedure. There is a correlation between asplenia/hyposplenia and the occurrence of fulminant sepsis, underlining the importance of developing surgical methods for preserving splenic function. Both clinical and experimental studies have shown at least partial morphological and functional regeneration of autogenic splenic implants (ASIs). Experimental studies investigating the immunoprotective effect of ASIs, based mostly on exposure of animals to various bacteria, have demonstrated that ASIs can increase the rate of bacterial clearance and decrease mortality from sepsis. Clinical studies have shown their ability to remove colloidal substances and altered erythrocyte corpuscular inclusions, such as Howell-Jolly, Heinz and Pappenheimer bodies, from the bloodstream. In this experimental study the functional and morphological regeneration of ASIs was studied over time in rats.

Progression of anemia and its relationship with renal function, blood pressure, and erythropoietin in rats with chronic kidney disease

“…This was based on data provided by Charles River Laboratories International (Wilmington, MA, USA) as being the mean hematocrit for 8 –10‐week‐old Lewis male and female rats using samples from animals housed in their standard production setting . Wright–Giemsa‐stained blood smears were prepared for all samples and examined microscopically for abnormal RBC morphology such as target cells, RBC fragments, and RBC corpuscular inclusions such as Howell–Jolly bodies as indicators of splenic dysfunction . Smears were also examined for WBC morphology and WBC precursors, and a manual differential WBC count was performed ( n = 100 cells per smear) to confirm the automated differential WBC count results.…”

Spleen Organoid Units Generate Functional Human and Mouse Tissue-Engineered Spleen in a Murine Model

“…It is known that without residual spleen, Howell-Jolly bodies persist indefinitely. 19 In a study in male Wistar rats, Howell-Jolly bodies were no longer identified on blood smear when 22.5% of spleen mass was present. 19 Both mTESp and hTESp contained CD4-and CD11cpositive cells that also indicated donor origin with either lamin or GFP costaining.…”

“…19 In a study in male Wistar rats, Howell-Jolly bodies were no longer identified on blood smear when 22.5% of spleen mass was present. 19 Both mTESp and hTESp contained CD4-and CD11cpositive cells that also indicated donor origin with either lamin or GFP costaining. Although there are multiple cell types of myeloid and lymphoid lineage required for the function of innate and adaptive immunity, both T cells and dendritic cells are required for spleen function.…”

Alterations in splenic function and gene expression in mice with depressive-like behavior induced by exposure to corticosterone

“…When the spleen is absent or insufficient, the number of HJB containing erythrocytes is increased (29). In the present study, the mice in the depression model group exhibited higher counts of HJB, which indicated that splenic function was compromised.…”