Bioprocessing of citrus waste peel for induced pectinase production by Aspergillus niger; its purification and characterization

Abstract

Agro-industrial residues are primarily composed of complex polysaccharides that strengthen microbial growth for the production of industrially important enzymes. Pectinases are one of the most widely disseminated enzymes in bacteria, fungi and plants. Czapeck media supplemented with orange waste peel as carbon source under submerged fermentation process Aspergillus niger presenting the preeminent enzymatic production. On partial optimization culture showed the maximum enzyme yield (117.1 ± 3.4 mM/mL/ min) at 30 C in an orange waste peel medium having pH 5.5 and substrate concentration (4%) after 5th day of fermentation. The produced enzyme was purified by ammonium sulfate precipitation and ion exchange chromatography. A purification fold of 5.59 with specific activity and % recovery of 97.2 U/mg and 12.96% was achieved respectively after gel filtration chromatographic technique. The molecular weight of purified pectinase from A. niger was 30 kDa evidenced by SDS-PAGE. Pectinase activity profile showed purified enzyme was optimally active at pH ¼ 7 and 55 C. The maximum production of pectinase in the presence of cheaper substrate at low concentration makes the enzyme useful in industrial sectors especially for textile and juice industry.

Partial purification and Characterization of Two Pectinases (Polygalacturonase and Pectin lyase) from Papaya Pericarp (Carica papaya cv. solo 8)

“…The increase in the specific activity of polygalacturonase (2.8 UI/mg) and pectin lyase (0.14 UI/mg) after dialysis could be explained by a loss of undesired proteins which interfere with the activity of pectinases (Undenwobele et al, 2014). These two first steps of purification (ammonium sulphate precipitation and dialysis) of enzymes have already been reported by Ahmed et al, (2016) in the purification of vegetable pectinases. The last step in the purification (Isoelectric focusing) of the polygalacturonase and pectin lyase isolated from the papaya pericarp cv.…”

Production of α-1,4-glucosidase from Bacillus licheniformis KIBGE-IB4 by utilizing sweet potato peel

“…Low concentration of substrate N . D N.D indicates that activity is not detected a Submerged fermentation was carried out using 20.0 g l −1 of substrates with pH 7.0 at 40°C for 48 h. The data presented are the mean values of three replicates with standard deviations than optimum is mostly insufficient to fulfill the energy demand of bacterial culture and to induce the bacterium for maximum enzyme synthesis (Ahmed et al 2015). The total carbohydrate content of sweet potato peel was also determined, and it was observed that sweet potato peel contains 0.0015/0.1 g total sugar content.…”

Valorization of Waste From Agro-Industry Using Thermo-Chemical Treatments

“…In general, the volatile content of the feed marks the yield of the reaction; furthermore, if the ash content is high the yield of the treatment will decrease. For the orange peel, the initial ash percentage reported by Ahmed et al [14], was 7.34%; while the initial ash content for the CH is non-existent according to studies carried out by Bondesson [15].…”