Bioprinted Chitosan and Hydroxyapatite Micro-Channels Structures Scaffold for Vascularization of Bone Regeneration

Shuai, Shuai; Qingxi,; Sun, Dong; Chen, Guihai; Yuanshao,; Liu, Ming; Yuanyuan,; A, Yu; Li, Qing; Hu$^{, }$; Jiang, Jiang

doi:10.1166/jbt.2017.1535

Cited by 13 publications

(8 citation statements)

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“…In this way, the coaxial extrusion of calcium chloride and sodium alginate is used to form hollow fibers . Moreover, biocompatible materials with different forming performances to create hollow fibers, such as sodium tripolyphosphate and chitosan, have been used to replace calcium chloride and sodium alginate. Although the approach of directly printing hollow fibers is well established, this method is limited to the unitary diameter of hollow fibers and planar footprints, and the scalability and spatiality of the engineered scaffolds are restricted.…”

Section: Introductionmentioning

confidence: 99%

A Facile Strategy for Fabricating Tissue Engineering Scaffolds with Sophisticated Prevascularized Networks for Bulk Tissue Regeneration

Liu

Zhang

et al. 2019

Macro Materials & Eng

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Despite the fact that the field of tissue engineering has had considerable advances over the past two decades, a series of unsolved problems still remain. Vascularization is one of the most important factors that greatly influence the function and size of the engineered scaffolds, which limits the clinical applications. In this work, a facile extracted molding method is presented for fabricating bulk tissue scaffolds with spatial networks. Briefly, the branched templates are designed, coated with paraffin on the surface, immersed into the mixture of microbial transglutaminase and gelatin, and extracted from fully enzymatic cross‐linking gelatin. The perfusion test is done and the mechanical properties of the scaffolds are investigated. Furthermore, in vitro and in vivo experiments demonstrate the nontoxicity and biocompatibility of the materials and fabrication process. Thus, this approach has great potential to overcome the challenge of rapid oxygen and nutrient delivery to engineered vascularized tissues implanted in vivo, opening the way to clinical applications.

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Section: Introductionmentioning

confidence: 99%

A Facile Strategy for Fabricating Tissue Engineering Scaffolds with Sophisticated Prevascularized Networks for Bulk Tissue Regeneration

Liu

Zhang

et al. 2019

Macro Materials & Eng

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“…Building on this method, water-soluble polymer poly(vinyl alcohol) (PVA) can be added into the sodium alginate to create 3D porous scaffolds with regular macropores and an artificial vasculature-like system . Moreover, biocompatible materials with different forming performances, such as chitosan and sodium tripolyphosphate, have been used to replace sodium alginate and calcium chloride . Although the use of hollow fibers is well-established, this method is generally restricted to planar footprints, and the final shape of the channels in the engineered scaffolds is limited.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Engineered Scaffolds with Spatial Prevascularized Networks for Bulk Tissue Regeneration

Zhang

et al. 2017

ACS Biomater. Sci. Eng.

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Despite significant progress in the fabrication of prevascularized networks over the past decade, a number of challenges remain. One of the most relevant issues is the lack of three-dimensional (3D) structures, which limits the clinical applications of the engineered scaffolds. Another problem is the complexity of prevascularized networks in engineered scaffolds, which is still less than that of human tissues, especially in the case of mature and bulk tissues. Thus, there is still the need to develop more flexible methods to better simulate the structure of natural tissues. In this work, we used a versatile sacrificial template method to fabricate bulk scaffolds with spatial prevascularized networks. Soft poly(vinyl alcohol) (PVA) filaments were used to print the sacrificial template, and the receiving platform was a stepped shaft, allowing the sacrificial template to have a complex 3D structure. The obtained template was embedded into gelatin and microbial transglutaminase (mTG). The inner PVA template could be extracted from the enzymatic cross-linking system, and an engineered scaffold with spatial prevascularized networks was obtained. In vitro experiments demonstrated that the fabrication process is biocompatible with cells.

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“…Chitosan/allyl bromide produced via a solvent free process was used for TPP at a resolution of 400 nm [228]. Besides the various fabrication techniques, chitosan can be combined with bioceramic hydroxyapatite to print hollow 3D scaffolds for improved vascularization and mechanical properties appropriate for bone tissue engineering [229].…”

Section: Structural and Mechanical Propertiesmentioning

confidence: 99%

“…Hyaluronic acid (4.3.1) [207][208][209]; Inclusion of GelMA, feature size 500 µm [210]; Cryogel E: 2-2.5 kPa [211]; Post-curing via UV, E: 1.3-10.6 kPa [213] Feature size 300 µm (SLA) [214]; Compressive E: 780 kPa [215] Cartilage tissue engineering and human adipose stem cells [215]; stromal cell elongation and drug screening [209]; retinal cell culturing [216]; hMSCs [217]; human adipose progenitor and stromal cells [211]; Schwann cells [219] Chitosan (4.3.2) [222,223]; Feature size 50 µm [224] [225,226]; Feature size 50 µm (SLA), E: 160-680 kPa [173]; Feature size 400 nm (TPP) [228]; Inclusion of HA [229] Anti-bacterial [230,231]; wound dressings [232]; skin tissue engineering [231]; bone tissue engineering [229]; pluripotent stem cells for neural tissue engineering [233]; articular cartilage tissue engineering [234]; skin constructs [235] Alginate (4.3.3)…”

Section: ) Applicationsmentioning

confidence: 99%

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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Bioprinted Chitosan and Hydroxyapatite Micro-Channels Structures Scaffold for Vascularization of Bone Regeneration

Cited by 13 publications

References 0 publications

A Facile Strategy for Fabricating Tissue Engineering Scaffolds with Sophisticated Prevascularized Networks for Bulk Tissue Regeneration

A Facile Strategy for Fabricating Tissue Engineering Scaffolds with Sophisticated Prevascularized Networks for Bulk Tissue Regeneration

Characterization of Engineered Scaffolds with Spatial Prevascularized Networks for Bulk Tissue Regeneration

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

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