A Facile Strategy for Fabricating Tissue Engineering Scaffolds with Sophisticated Prevascularized Networks for Bulk Tissue Regeneration

Liu, Suihong; Zhang, Haiguang; Li, Shuai; Sun, Chengyan; Hu, Qingxi

doi:10.1002/mame.201800642

Cited by 11 publications

(7 citation statements)

References 54 publications

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“…The data are corroborated well in accordance with earlier studies (Li et al, 2017; S.‐H. Liu et al, 2019). As for the tensile strength, the stress and strain of A‐SA‐Gel hydrogel samples were higher than that of SA/Gel sample.…”

Section: Discussionsupporting

confidence: 92%

3D printing of self‐standing and vascular supportive multimaterial hydrogel structures for organ engineering

Liu

Shen

et al. 2021

Biotech & Bioengineering

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Three dimensional printable formulation of self‐standing and vascular‐supportive structures using multi‐materials suitable for organ engineering is of great importance and highly challengeable, but, it could advance the 3D printing scenario from printable shape to functional unit of human body. In this study, the authors report a 3D printable formulation of such self‐standing and vascular‐supportive structures using an in‐house formulated multi‐material combination of albumen/alginate/gelatin‐based hydrogel. The rheological properties and relaxation behavior of hydrogels were analyzed before the printing process. The suitability of the hydrogel in 3D printing of various customizable and self‐standing structures, including a human ear model, was examined by extrusion‐based 3D printing. The structural, mechanical, and physicochemical properties of the printed scaffolds were studied systematically. Results supported the 3D printability of the formulated hydrogel with self‐standing structures, which are customizable to a specific need. In vitro cell experiment showed that the formulated hydrogel has excellent biocompatibility and vascular supportive behavior with the extent of endothelial sprout formation when tested with human umbilical vein endothelial cells. In conclusion, the present study demonstrated the suitability of the extrusion‐based 3D printing technique for manufacturing complex shapes and structures using multi‐materials with high fidelity, which have great potential in organ engineering.

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Section: Discussionsupporting

confidence: 92%

3D printing of self‐standing and vascular supportive multimaterial hydrogel structures for organ engineering

Liu

Shen

et al. 2021

Biotech & Bioengineering

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“…In addition, to further observe the cell morphology on the scaffold, nucleus/cytoskeleton fluorescent staining was used as reported in previous research. [55] Briefly, tetramethylrhodamine (TRITC)-Phalloidin and DAPI (Maokang, Shanghai, China) were used to stain the cytoskeleton and nucleus of cells, respectively. After culturing 3 d, the cell-seeded scaffolds were taken out and rinsed slightly three times with PBS for 30 s each time.…”

Section: Methodsmentioning

confidence: 99%

Alginate/Gelatin‐Based Hydrogel with Soy Protein/Peptide Powder for 3D Printing Tissue‐Engineering Scaffolds to Promote Angiogenesis

Liu

Dong

et al. 2022

Macromolecular Bioscience

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In recent years, 3D bioprinting has attracted broad research interest in biomedical engineering and clinical applications. However, there are two issues need to be solved urgently at present, the development of ink is the first pressing thing for 3D printing tissue engineering scaffold, other thing is the promotion of angiogenesis in the scaffold. Therefore, a gelatin/sodium alginate‐based hydrogel with protein‐rich is developed here, which is prepared by gelatin, sodium alginate, and soy protein/soy peptide powder. The prepared inks exhibit excellent shear‐thinning behavior, which contribute to extrusion‐based printing; also shown good crosslinking ability by calcium chloride. The macroporous composite scaffolds are printed by 3D printing using the developed ink and the physicochemical properties of the scaffolds are evaluated. Moreover, the cytocompatibility of printed scaffold is characterized by using human umbilical vein epidermal cells, results show that the scaffolds with soy protein and soy peptide powder can promote cell attach, spread, migration, and proliferation. The further research of chicken embryo allantoic membrane assay and animal experiment are carried, and results present that the scaffold can promote the growth of neo‐vessels in the scaffold, which means the developed ink with soy protein and soy peptide powder has great potential for angiogenesis.

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“…To compare the viability of cells cultured on the mTG-crosslinked vascular scaffold and glutaraldehyde-crosslinked scaffold, HUVECs (Zhongqiaoxinzhou Biotech Co., Ltd, Shanghai, China) were cultured on both scaffolds. 28–31…”

Section: Methodsmentioning

confidence: 99%

Preparation and optimization of a gelatin-based biomimetic three-layered vascular scaffold

Zhang

2019

J Biomater Appl

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Electrospinning is promising approach for producing biomimetic vascular scaffolds. In particular, gelatin-based electrospun scaffolds offer excellent biocompatibility. However, gelatin-based vascular scaffolds are difficult to remove from the collector after electrospinning and cross-linking agents such as glutaraldehyde, which is commonly used to improve the strength of water-soluble electrospun gelatin, are highly toxic. Herein, we present a novel electrospinning method for preparing three-layered vascular scaffolds. First, Tween 80 was added to 2,2,2-trifluoroethanol as the polymer electrospinning solution and the gelatin electrospinning solution was mixed with polylactic acid and polycaprolactone, respectively. The three-layered vascular scaffold was fabricated by electrospinning an inner layer of gelatin, a middle layer of polyester, and another outer layer of gelatin. Finally, the electrospun scaffolds were cross-linked with microbial transglutaminase and vacuum dried. Biomechanical properties of the scaffold were determined by tensile testing. Furthermore, the structure and biocompatibility of the scaffolds were assessed by hydrophilicity tests, scanning electron microscopy, and cell seeding experiments. Our results suggest the proposed electrospinning method is suitable for preparing biomimetic vascular scaffolds for cardiovascular tissue engineering applications. Moreover, this paper provides a useful reference for the preparation and optimization of vascular scaffolds.

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A Facile Strategy for Fabricating Tissue Engineering Scaffolds with Sophisticated Prevascularized Networks for Bulk Tissue Regeneration

Cited by 11 publications

References 54 publications

3D printing of self‐standing and vascular supportive multimaterial hydrogel structures for organ engineering

3D printing of self‐standing and vascular supportive multimaterial hydrogel structures for organ engineering

Alginate/Gelatin‐Based Hydrogel with Soy Protein/Peptide Powder for 3D Printing Tissue‐Engineering Scaffolds to Promote Angiogenesis

Preparation and optimization of a gelatin-based biomimetic three-layered vascular scaffold

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