Characterization of Engineered Scaffolds with Spatial Prevascularized Networks for Bulk Tissue Regeneration

Li, Shuai; Zhang, Hai-Guang; Li, Dongdong; Wu, Jianping; Sun, Chengyan; Hu, Qingxi

doi:10.1021/acsbiomaterials.7b00355

Cited by 9 publications

(10 citation statements)

References 45 publications

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“…The compressive strength and the strain of A‐SA‐Gel hydrogel sample exceeded 6 MPa and 55%, respectively, and was higher than SA/Gel sample (see Figure 6). The data are corroborated well in accordance with earlier studies (Li et al, 2017; S.‐H. Liu et al, 2019).…”

Section: Discussionsupporting

confidence: 92%

“…Therefore, it is necessary to improve its printability and bioactive functional properties suitable for engineering tissues and organs. Gelatin is a highly cell compatible and biologically active polymer derived from collagen by partial hydrolysis (Li et al, 2017). It is also widely used as a bioink either as such or in combination with other biomaterials, including alginate, owing to its reversible thermal‐dynamic trait (Djabourov et al, 1988a).…”

Section: Discussionmentioning

confidence: 99%

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3D printing of self‐standing and vascular supportive multimaterial hydrogel structures for organ engineering

Liu

Shen

et al. 2021

Biotech & Bioengineering

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Three dimensional printable formulation of self‐standing and vascular‐supportive structures using multi‐materials suitable for organ engineering is of great importance and highly challengeable, but, it could advance the 3D printing scenario from printable shape to functional unit of human body. In this study, the authors report a 3D printable formulation of such self‐standing and vascular‐supportive structures using an in‐house formulated multi‐material combination of albumen/alginate/gelatin‐based hydrogel. The rheological properties and relaxation behavior of hydrogels were analyzed before the printing process. The suitability of the hydrogel in 3D printing of various customizable and self‐standing structures, including a human ear model, was examined by extrusion‐based 3D printing. The structural, mechanical, and physicochemical properties of the printed scaffolds were studied systematically. Results supported the 3D printability of the formulated hydrogel with self‐standing structures, which are customizable to a specific need. In vitro cell experiment showed that the formulated hydrogel has excellent biocompatibility and vascular supportive behavior with the extent of endothelial sprout formation when tested with human umbilical vein endothelial cells. In conclusion, the present study demonstrated the suitability of the extrusion‐based 3D printing technique for manufacturing complex shapes and structures using multi‐materials with high fidelity, which have great potential in organ engineering.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

3D printing of self‐standing and vascular supportive multimaterial hydrogel structures for organ engineering

Liu

Shen

et al. 2021

Biotech & Bioengineering

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“…The representative compressive process is illustrated in Figure . The maximum compressive strength (four‐level branched spatial structures, as shown in Figure d) was recorded as 60% strain according to our previous research (almost all of the tested samples were fractured at 60% strain). Young's modulus (four‐level branched spatial structures, as shown in Figure d) was recorded as a measure of the rigidity of the scaffolds.…”

Section: Resultsmentioning

confidence: 74%

“…The technique is based on a different receiving platform to form different template, and then the template was sacrified or extracted to form channel. In our previous study, printing poly(vinyl alcohol) (PVA) filaments on a cylinder and a stepped shaft were used to fabricate sacrificial template, and the engineered scaffolds with spatial prevascularized networks were obtained by sacrificing and extracting the printed sacrificial PVA template. Although 3D branched connected channel in the scaffold was quickly acquired, this method is generally restricted to materials and channel‐scale.…”

Section: Introductionmentioning

confidence: 99%

A Facile Strategy for Fabricating Tissue Engineering Scaffolds with Sophisticated Prevascularized Networks for Bulk Tissue Regeneration

Liu

Zhang

et al. 2019

Macro Materials & Eng

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Despite the fact that the field of tissue engineering has had considerable advances over the past two decades, a series of unsolved problems still remain. Vascularization is one of the most important factors that greatly influence the function and size of the engineered scaffolds, which limits the clinical applications. In this work, a facile extracted molding method is presented for fabricating bulk tissue scaffolds with spatial networks. Briefly, the branched templates are designed, coated with paraffin on the surface, immersed into the mixture of microbial transglutaminase and gelatin, and extracted from fully enzymatic cross‐linking gelatin. The perfusion test is done and the mechanical properties of the scaffolds are investigated. Furthermore, in vitro and in vivo experiments demonstrate the nontoxicity and biocompatibility of the materials and fabrication process. Thus, this approach has great potential to overcome the challenge of rapid oxygen and nutrient delivery to engineered vascularized tissues implanted in vivo, opening the way to clinical applications.

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“…To compare the viability of cells cultured on the mTG-crosslinked vascular scaffold and glutaraldehyde-crosslinked scaffold, HUVECs (Zhongqiaoxinzhou Biotech Co., Ltd, Shanghai, China) were cultured on both scaffolds. 28–31…”

Section: Methodsmentioning

confidence: 99%

Preparation and optimization of a gelatin-based biomimetic three-layered vascular scaffold

Zhang

2019

J Biomater Appl

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Electrospinning is promising approach for producing biomimetic vascular scaffolds. In particular, gelatin-based electrospun scaffolds offer excellent biocompatibility. However, gelatin-based vascular scaffolds are difficult to remove from the collector after electrospinning and cross-linking agents such as glutaraldehyde, which is commonly used to improve the strength of water-soluble electrospun gelatin, are highly toxic. Herein, we present a novel electrospinning method for preparing three-layered vascular scaffolds. First, Tween 80 was added to 2,2,2-trifluoroethanol as the polymer electrospinning solution and the gelatin electrospinning solution was mixed with polylactic acid and polycaprolactone, respectively. The three-layered vascular scaffold was fabricated by electrospinning an inner layer of gelatin, a middle layer of polyester, and another outer layer of gelatin. Finally, the electrospun scaffolds were cross-linked with microbial transglutaminase and vacuum dried. Biomechanical properties of the scaffold were determined by tensile testing. Furthermore, the structure and biocompatibility of the scaffolds were assessed by hydrophilicity tests, scanning electron microscopy, and cell seeding experiments. Our results suggest the proposed electrospinning method is suitable for preparing biomimetic vascular scaffolds for cardiovascular tissue engineering applications. Moreover, this paper provides a useful reference for the preparation and optimization of vascular scaffolds.

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Characterization of Engineered Scaffolds with Spatial Prevascularized Networks for Bulk Tissue Regeneration

Cited by 9 publications

References 45 publications

3D printing of self‐standing and vascular supportive multimaterial hydrogel structures for organ engineering

3D printing of self‐standing and vascular supportive multimaterial hydrogel structures for organ engineering

A Facile Strategy for Fabricating Tissue Engineering Scaffolds with Sophisticated Prevascularized Networks for Bulk Tissue Regeneration

Preparation and optimization of a gelatin-based biomimetic three-layered vascular scaffold

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