Biomimetic nanofibrous scaffolds: Preparation and characterization of chitin/silk fibroin blend nanofibers

Park, Ko Eun; Jung, Sung Youn; Lee, Seung Jin; Min, Byung‐Moo; Park, Won Ho

doi:10.1016/j.ijbiomac.2006.03.003

Cited by 169 publications

(67 citation statements)

References 35 publications

(43 reference statements)

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“…27 The solvent of the SF sponge, 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), was purchased from Acros Organics (Belgium) and used as received. PCL (M n =75,000) was purchased from Dow Chemical (Tone 767; USA).…”

Section: Methodsmentioning

confidence: 99%

Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds

Kim¹,

Park²,

Kim³

et al. 2015

IJN

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The broad application of electrospun nanofibrous scaffolds in tissue engineering is limited by their small pore size, which has a negative influence on cell migration. This disadvantage could be significantly improved through the combination of nano- and microfibrous structure. To accomplish this, different nano/microfibrous scaffolds were produced by hybrid electrospinning, combining solution electrospinning with melt electrospinning, while varying the content of the nanofiber. The morphology of the silk fibroin (SF)/poly(ε-caprolactone) (PCL) nano/microfibrous composite scaffolds was investigated with field-emission scanning electron microscopy, while the mechanical and pore properties were assessed by measurement of tensile strength and mercury porosimetry. To assay cell proliferation, cell viability, and infiltration ability, human mesenchymal stem cells were seeded on the SF/PCL nano/microfibrous composite scaffolds. From in vivo tests, it was found that the bone-regenerating ability of SF/PCL nano/microfibrous composite scaffolds was closely associated with the nanofiber content in the composite scaffolds. In conclusion, this approach of controlling the nanofiber content in SF/PCL nano/microfibrous composite scaffolds could be useful in the design of novel scaffolds for tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds

Kim¹,

Park²,

Kim³

et al. 2015

IJN

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“…70,120 This electrospun silk fibrin scaffold promotes osteoblast proliferation and increases osteoblastic alkaline phosphatase activity. 90 Many scaffold materials have been used as well: polyethylene glycol, 53,78,111 nano-HA membranes, 124 poly b-amino esters, 10 injectable chitosan gel, minimally invasive tissue-engineered bone, 107 and others. An exhaustive evaluation of current and future scaffold materials is available in the literature and is beyond the scope of this article.…”

Section: Advanced Scaffold Materialsmentioning

confidence: 99%

Cranial bone defects: current and future strategies

Szpalski¹,

Barr²,

Wetterau³

et al. 2010

FOC

173

115

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Bony defects in the craniomaxillofacial skeleton remain a major and challenging health concern. Surgeons have been trying for centuries to restore functionality and aesthetic appearance using autografts, allografts, and even xenografts without entirely satisfactory results. As a result, physicians, scientists, and engineers have been trying for the past few decades to develop new techniques to improve bone growth and bone healing. In this review, the authors summarize the advantages and limitations of current animal models; describe current materials used as scaffolds, cell-based, and protein-based therapies; and lastly highlight areas for future investigation. The purpose of this review is to highlight the major scaffold-, cell-, and protein-based preclinical tools that are currently being developed to repair cranial defects.

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“…This may be a consequence of the high surface area available for cell attachment due to their three-dimensional features and high surface area-to volume ratios, which are favorable parameters for cell attachment, growth, and proliferation. Cell studies conducted on chitin/PGA (Park et al, 2006a) and chitin/SF (Park et al, 2006b) fibrous mats proved that a matrix consisting of 25% PGA or SF and 75% chitin had the best results. The chitin/PGA fibers had a bovine serum albumin coating and were considered a good candidate for use as a tissue-engineering scaffold because normal human epidermal fibroblasts (NHEF) attached and spread.…”

Section: Applications Of Chitin and Chitosan Nano-fibers In Tissue-enmentioning

confidence: 99%

“…In vitro degradation studies, conducted in phosphate-buffered saline (PBS), pH 7.2 demonstrated that the blend fibers degraded faster than pure PGA fibers. Also, electrospun chitin/silk fibroin (SF) nanofibers in HFIP solvent was reported (Park et al, 2006b). The average diameters of chitin/SF blend fibers decreased from 920 to 340 nm, with the increase of chitin content in blend compositions.…”

Section: Introductionmentioning

confidence: 99%

Perspectives of Chitin and Chitosan Nanofibrous Scaffolds in Tissue Engineering

Jayakumar¹,

Nair²,

Furuike³

et al. 2010

Tissue Engineering

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Chitin and its deacetylated derivative, chitosan, are non-toxic, biodegradable biopolymers currently being developed for use in biomedical applications such as tissue engineering scaffolds, wound dressings, separation membranes, antibacterial coatings, stent coatings, and sensors. Recently, nano fibrous scaffolds based on chitin or chitosan have potential applications in tissue engineering. Tissue engineering is one of the most exciting interdisciplinary and multidisciplinary research areas today, and there has been exponential growth in the number of research publications in this area in recent years. It involves the use of living cells, manipulated through their extracellular environment or genetically to develop biological substitutes for implantation into the body and/or to foster remodeling of tissues in some active manners. Electrospun chitin and chitosan nano fibrous scaffolds would be used to produce tissue engineering scaffolds with improved cytocompatibility, which could mimic the native extracellular matrix (ECM). Electrospinning is truly a feasible means of producing nano fibrous scaffolds that resemble the ECM, however, moreover than this, it is imperative that the effects of an artificial matrix has on cell growth, proliferation, and differentiation. This review summarizes the recent progress in chitin and chitosan based nano fibrous scaffolds with an emphasis in tissue engineering applications.

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Biomimetic nanofibrous scaffolds: Preparation and characterization of chitin/silk fibroin blend nanofibers

Cited by 169 publications

References 35 publications

Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds

Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds

Cranial bone defects: current and future strategies

Perspectives of Chitin and Chitosan Nanofibrous Scaffolds in Tissue Engineering

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Biomimetic nanofibrous scaffolds: Preparation and characterization of chitin/silk fibroin blend nanofibers

Cited by 169 publications

References 35 publications

Effect of nanofiber content on bone regeneration of silk fibroin/poly(&epsilon;-caprolactone) nano/microfibrous composite scaffolds

Effect of nanofiber content on bone regeneration of silk fibroin/poly(&epsilon;-caprolactone) nano/microfibrous composite scaffolds

Cranial bone defects: current and future strategies

Perspectives of Chitin and Chitosan Nanofibrous Scaffolds in Tissue Engineering

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Product

Resources

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Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds

Effect of nanofiber content on bone regeneration of silk fibroin/poly(ε-caprolactone) nano/microfibrous composite scaffolds