Biomimetic behavior and nanomolar detection of hydrogen peroxide on an electrochemically pre-treated hematin modified glassy carbon electrode

Buoro, Rafael M.; Bacil, Raphael P.; Sanz, Caroline G.; Campos, Othon Souto; Serrano, Sı́lvia Helena Pires

doi:10.1016/j.snb.2017.03.176

Cited by 11 publications

(3 citation statements)

References 41 publications

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“…Meanwhile, recently, a bicarbonate solution preanodized GCE that has specific pores on the surface was reported for hematin immobilization. 26 This system showed E°′ = −0.3 V versus Ag/AgCl and Γ Hemat = 0.9 × 10 −10 mol cm −2 . Interestingly, a new hematin immobilization method introduced in this work based on GMC and chitosan, which can be prepared within 15 ± 1 min, showed excellent biomimicking redox signal at E°′ = −0.390 V versus Ag/AgCl with the highest Γ Hemat = 126 × 10 −10 mol cm −2 (active site loading) suitable to perform various electratalytic applications.…”

Section: Introductionmentioning

confidence: 92%

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Highly Redox-Active Hematin-Functionalized Carbon Mesoporous Nanomaterial for Electrocatalytic Reduction Applications in Neutral Media

Amreen

Kumar

2018

ACS Appl. Nano Mater.

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Hematin is a hydroxyl group linked heme site (hydroxyl heme) of the natural enzymes/proteins like hemoglobin, cytochrome c, catalase, and horseradish peroxidase, and it has an important role in the physiological function. Because of problems like poor electron-transfer functionality (on solid electrodes), poor solubility, and molecular aggregation in aqueous solution, limited electrochemical studies have been reported in the literature. A new electrode modification method for hematin using graphitized mesoporous carbon nanomaterial and chitosan for enhanced redox-active and efficient electrocatalytic reductions of hydrogen peroxide and dissolved oxygen in neutral pH was demonstrated in this work. The hematin-modified electrode showed a highly stable redox peak at E°′ = −0.390 V versus Ag/AgCl with a heterogeneous rate constant value of 1.34 s–1. Calculated hematin-active loading concentration (Γhemat = 126 × 10–10 mol cm–2) is ∼20 times higher than the reported values. Physicochemical and electrochemical characterizations revealed trapping of the hematin via axial bond coordination and intermolecular hydrogen bonding with amino functional groups of chitosan and π–π interactions with the graphitic site of mesoporous carbon within the matrix. The new hematin electrode showed ∼400 mV reduction in the overpotential with current sensitivity/detection range of 570 nA μM–1/100–900 μM and 6.7 μA ppm–1/1–10 ppm, respectively, for H2O2 and dissolved oxygen reduction reactions in pH 7 phosphate buffer solution. Michaelis–Menten kinetics were applied for the H2O2 reduction reaction and estimated the rate constant values as K M = 0.78 mM and k s = 1.15 s–1. No marked interference was noticed with common biochemicals such as nitrite, nitrate, glucose, uric acid, ascorbic acid, xanthine, hypoxanthine, cysteine, and dopamine on amperometric i–t detection of H2O2 indicating the activity similar to the heme-based proteins/enzymes.

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Section: Introductionmentioning

confidence: 92%

“…In general, these kinds of preparations require laborious multiple coatings and immersion procedures. Meanwhile, recently, a bicarbonate solution preanodized GCE that has specific pores on the surface was reported for hematin immobilization . This system showed E °′ = −0.3 V versus Ag/AgCl and Γ Hemat = 0.9 × 10 –10 mol cm –2 .…”

Section: Introductionmentioning

confidence: 99%

Highly Redox-Active Hematin-Functionalized Carbon Mesoporous Nanomaterial for Electrocatalytic Reduction Applications in Neutral Media

Amreen

Kumar

2018

ACS Appl. Nano Mater.

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“…As FTAL-M, principalmente de Fe (II), Cu (II), Co (II), Mn (II), e, também, a porfirina, HEM, são conhecidas como agentes que catalisam uma diversidade de reações redox. Estes complexos são reconhecidos por sua excelente atividade eletrocatalítica em muitas reações, que são dependentes do átomo central (PENG et al, 2020;LIU et al, 2019;BUORO et al, 2017).…”

Section: Ftalocianinas Metálicas E Heminaunclassified

Estudo eletroquímico de compostos nitro-heterocíclicos potencialmente antichagásicos utilizando eletrodos quimicamente modificados com ftalocianinas

Brito¹

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Gold nanocluster-based ratiometric fluorescent probes for hydrogen peroxide and enzymatic sensing of uric acid

Yang

Luo

et al. 2018

Microchim Acta

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A method is described for ratiometric fluorometric assays of HO by using two probes that have distinct response profiles. Under the catalytic action of ferrous ion, the 615 nm emission of protein-stabilized gold nanoclusters (under 365 nm photoexcitation) is quenched by HO, while an increased signal is generated with a peak at 450 nm by oxidizing coumarin with the HO/Fe(II) system to form a blue emitting fluorophore. These decrease/increase responses give a ratiometric signal. The ratio of the fluorescences at the two peaks are linearly related to the concentration of HO in the range from 0.05 to 10 μM, with a 7.7 nM limit of detection. The detection scheme was further coupled to the urate oxidase catalyzed oxidation of uric acid which proceeds under the formation of HO. This method provides an simple and effective means for the construction of ratiometric fluorometric (enzymatic) assays that involve the detection of HO. Graphical abstract Under catalysis by ferrous ion, hydrogen peroxide quenches the luminescence of gold nanoclusters (AuNCs) and oxidizes coumarin into a fluorescent derivative, which rendered fluorescence ON and OFF at two distinct wavelengths for ratiometric measurements.

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Biomimetic behavior and nanomolar detection of hydrogen peroxide on an electrochemically pre-treated hematin modified glassy carbon electrode

Cited by 11 publications

References 41 publications

Highly Redox-Active Hematin-Functionalized Carbon Mesoporous Nanomaterial for Electrocatalytic Reduction Applications in Neutral Media

Highly Redox-Active Hematin-Functionalized Carbon Mesoporous Nanomaterial for Electrocatalytic Reduction Applications in Neutral Media

Estudo eletroquímico de compostos nitro-heterocíclicos potencialmente antichagásicos utilizando eletrodos quimicamente modificados com ftalocianinas

Gold nanocluster-based ratiometric fluorescent probes for hydrogen peroxide and enzymatic sensing of uric acid

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