Gold nanocluster-based ratiometric fluorescent probes for hydrogen peroxide and enzymatic sensing of uric acid

Yang, Dongqin; Luo, Minchuan; Di, Junwei; Tu, Yifeng; Yan, Jilin

doi:10.1007/s00604-018-2823-5

Cited by 37 publications

(4 citation statements)

References 33 publications

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“…H 2 O 2 has been reported to be a weak fluorescence quencher of AuNCs in previous literature. 55 The Fmoc-FF/AuNCs/HRP films showed a luminescence decrease phenomenon after dipping into pH 5.0 buffers containing H 2 O 2 (Figure 5A). The FL 660 from the films gradually declined as the H 2 O 2 concentration (c H 2 O 2 ) was increased from 0 mM to 2.0 mM and reached a plateau at 2.0 mM.…”

Section: Resultsmentioning

confidence: 99%

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Constructing a Facile Biocomputing Platform Based on Smart Supramolecular Hydrogel Film Electrodes with Immobilized Enzymes and Gold Nanoclusters

Cong

Wei

et al. 2021

ACS Appl. Mater. Interfaces

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Herein, fluorescent gold nanoclusters (AuNCs) and horseradish peroxidase (HRP) were simultaneously embedded into self-assembled dipeptide supramolecular films of N-fluorenylmethoxycarbonyl diphenylalanine (Fmoc-FF) on the surface of ITO electrodes (Fmoc-FF/AuNCs/HRP) by using a simple single-step process. In the films, both the fluorescence property of AuNCs and the bioelectrocatalytic property of HRP were well maintained and could be reversibly regulated by pH-sensitive structural changes in the Fmoc-FF hydrogel films. Cu(II)/EDTA in the solution could lead to the aggregation/disaggregation of AuNCs and further quenching/dequenching the fluorescence signal from the films. Meanwhile, the blue complexes formed by Cu(II) and EDTA could produce a UV–vis signal in the solution. In addition, the coordinated Cu(II) in the films enhanced the electrocatalytic capacity toward the reduction of H2O2 and could switch the current signal. A biomolecular logic circuit was built based on the smart film electrode system by using pH, the concentrations of EDTA, Cu(II) and H2O2 as inputs, while the fluorescence intensity (FL), current (I) and UV–vis extinction (E) of the solution as outputs. Various logic devices were fabricated using the uniform platform, consisting of an encoder/decoder, demultiplexer, dual-transfer gate, keypad lock, digital comparator, half adder, and controlled NOT (CNOT) gate. Specifically, an electronic three-value logic gate, gullibility (ANY) gate, was first mimicked in this biocomputing system. This work not only demonstrated the construction of a new type of multivalued logic gate by using a dipeptide micromolecular matrix but also provided a new approach for designing sophisticated biologic functions, establishing smart multianalyte biosensing or fabricating biology information processing through the use of a simple film system.

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Section: Resultsmentioning

confidence: 99%

“…H 2 O 2 has been reported to be a weak fluorescence quencher of AuNCs in previous literature . The Fmoc-FF/AuNCs/HRP films showed a luminescence decrease phenomenon after dipping into pH 5.0 buffers containing H 2 O 2 (Figure A).…”

Section: Resultsmentioning

confidence: 99%

Constructing a Facile Biocomputing Platform Based on Smart Supramolecular Hydrogel Film Electrodes with Immobilized Enzymes and Gold Nanoclusters

Cong

Wei

et al. 2021

ACS Appl. Mater. Interfaces

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“…Apart from fluorescence quenching and enhancement, another strategy for biosensing is based on ratiometric fluorescence (RF)/color change [ 145 , 146 , 147 ]. In this strategy ( Figure 8 c), the protein-templated metal NC is typically used in conjunction with a secondary probe emitting at a different wavelength and, thus, a different emission color [ 133 , 148 , 149 ]. The analyte interacts with the protein-templated metal NC to quench its emission, leaving only the emission of the second emitter.…”

Section: Biomedical Applications Of Protein-templated Metal Nanoparti...mentioning

confidence: 99%

Protein-Templated Metal Nanoclusters: Molecular-like Hybrids for Biosensing, Diagnostics and Pharmaceutics

Tan

Wang

et al. 2023

Molecules

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The use of proteins as biomolecular templates to synthesize atomically precise metal nanoclusters has been gaining traction due to their appealing properties such as photoluminescence, good colloidal- and photostability and biocompatibility. The synergistic effect of using a protein scaffold and metal nanoclusters makes it especially attractive for biomedical applications. Unlike other reviews, we focus on proteins in general as the protective ligand for various metal nanoclusters and highlight their applications in the biomedical field. We first introduce the approaches and underlined principles in synthesizing protein-templated metal nanoclusters and summarize some of the typical proteins that have been used thus far. Afterwards, we highlight the key physicochemical properties and the characterization techniques commonly used for the size, structure and optical properties of protein-templated metal nanoclusters. We feature two case studies to illustrate the importance of combining these characterization techniques to elucidate the formation process of protein-templated metal nanoclusters. Lastly, we highlight the promising applications of protein-templated metal nanoclusters in three areas—biosensing, diagnostics and therapeutics.

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“…The fluorescence changes of F 450 / F 640 had fantastic linearity to H 2 O 2 concentrations in the range of 0.8−200 µmol L −1 ( R 2 = 0.9993), with LOD of 0.2 µmol L −1 . In another study, Au NCs‐based ratiometric fluorescent probes were synthesized for probing H 2 O 2 and proved to be dual emissive by Yan group . In the presence of ferrous ion as catalytic agent, the emission of protein‐stabilized Au NCs at 615 nm was quenched by H 2 O 2 , whereas the blue emission at 450 nm was enhanced due to oxidizing coumarin with the H 2 O 2 /Fe(II) system.…”

Section: Sensing Applicationsmentioning

confidence: 99%

Metal Nanoclusters–Based Ratiometric Fluorescent Probes from Design to Sensing Applications

Zhao

Wang

et al. 2019

Part & Part Syst Charact

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The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/ppsc.201900298. Ratiometric fluorescent probes have many advantages including improved sensitivity, high reliability and accuracy, visualization sensing, etc. Herein, the development of metal nanoclusters (NCs)-based ratiometric fluorescent probes in the recent years is summarized. NCs, an emerging new class of fluorescent nanomaterials, have demonstrated excellent optical properties, good biocompatibility, great aqueous solubility, low cost, and simple synthesis, and NCs-based ratiometric fluorescent probes have attracted much attention. In this progress report, the preparation and properties of NCs and the design of ratiometric fluorescent probes are summarized. Sensing of a broad range of analytes including cations, gas, small molecules, macromolecules, temperature, and pH is discussed. In addition, the challenges and future directions for NCs-based ratiometric fluorescent probes are also presented.

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Gold nanocluster-based ratiometric fluorescent probes for hydrogen peroxide and enzymatic sensing of uric acid

Cited by 37 publications

References 33 publications

Constructing a Facile Biocomputing Platform Based on Smart Supramolecular Hydrogel Film Electrodes with Immobilized Enzymes and Gold Nanoclusters

Constructing a Facile Biocomputing Platform Based on Smart Supramolecular Hydrogel Film Electrodes with Immobilized Enzymes and Gold Nanoclusters

Protein-Templated Metal Nanoclusters: Molecular-like Hybrids for Biosensing, Diagnostics and Pharmaceutics

Metal Nanoclusters–Based Ratiometric Fluorescent Probes from Design to Sensing Applications

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