Biomedical polymer scaffolds mimicking bone marrow niches to advance in vitro expansion of hematopoietic stem cells

Abstract

Hematopoietic stem cells (HSCs) transplantation provides an effective platform for the treatment of hematological disorders. However, the donor shortage of HSCs and immune responses severely restrict the clinical applications of...

Bone marrow-inspired hydrogel/graphene composite scaffolds to support in vitro expansion of hematopoietic stem cells

“…10,11 Compared to traditional two-dimensional (2D) cell culture, a 3D culture system more realistically mimics the bone marrow niche, supporting the in vitro HSC culture. As a result, to reconstitute the features of the ECM in the bone marrow, many efforts have been devoted to building 3D scaffolds, [12][13][14] including hydrogels, fibrous and decellularized scaffolds fabricated based on ECM components, such as hyaluronic acid (HA) and collagen. 15,16 Of these scaffold biomaterials, hydrogels have gained considerable popularity in the engineering of functional tissues due to their swelling properties, biocompatibility, flexible synthesis and resemblance to soft tissue.…”