Biomass Production of Gynura procumbens Adventitious Roots in Different Type of Liquid Culture

Abstract

<p class="IsiAbstrakIndo"><em><span lang="EN-GB">Gynura procumbens</span></em><span lang="EN-GB"> has a potency to produce raw material for pharmaceutical industry. Liquid culture systems have significant effects on multiplication rates of organ, so the propagation technology to increase biomass of this plant or organ using the liquid culture system was necessary. This research was conducted to compare biomass production of adventitious roots of </span><em><span lang="EN-GB">G. procumbens</span></em><span lang="EN-GB"> in different liquid culture (shake flask, temporary immersion bioreactor and balloon-type bubble bioreactor). Adventitious roots culture was maintained in Murashige and Skoog liquid medium supplemented with IBA (indole butyric acid) 5 mg/L and different concentrations of sucrose (10, 30, 50 g/L). Result showed that the highest biomass production (fresh weight) was in shake flask that was 3.9-fold higher than initial explant, while in temporary immersion system was 5.12-fold higher than initial explant, and in balloon- type bubble bioreactor was 13.1-fold higher than initial explant. The highest enhancement of adventitious roots was occurred at sucrose supplementation of 50 g/L. Based on this research we found that the best type of liquid culture to increase biomass of </span><em><span lang="EN-GB">G. procumbens</span></em><span lang="EN-GB"> adventitious roots was balloon-type bubble bioreactor. Scaling-up of adventitious root culture are necessary to fulfill the need of raw material for pharmaceutical industry and ballon-type bubble bioreactor was the most suitable method to scale up the performance. </span></p>

Adventitious Root Culture—An Alternative Strategy for Secondary Metabolite Production: A Review

“…The bubbles of the balloon-type bubble bioreactor (BTBB) lessen the shear forces; therefore, this kind of bioreactor is suitable for culturing plant organs. However, the study conducted by Manuhara et al [141] showed that the highest biomass production (fresh weight) was found in the balloon-type bubble bioreactor (13.1-fold higher than the initial explant), followed by that in the temporary immersion system bioreactor (5.12-fold higher than the initial explant) and shake flask (3.9-fold higher than the initial explant), respectively [141].…”

“…In Gynura procumbens, the production of root biomass was compared to different liquid cultures (shake flask, balloon-type bubble bioreactor and temporary-immersion bioreactor). It has been reported that some problems in liquid culture, such as asphyxia and hyperhydricity, were observed during the low oxygen and submerged conditions in the medium [141]. However, to overcome this problem, a temporary immersion system bioreactor and balloon-type bubble bioreactor was applied.…”

Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

“…Some authors have monitored the effect of different sucrose concentrations in the culture substrate on biomass production. In particular, Curcuma zedoaria, Zingiber zerumbet, Gynura procumbens, and Bletilla striata have showed that a higher concentration of sugar improves the performance of explants [52,62,73], whereas a significant increase in shoots number (13.05) of Bambusa vulgaris was noted in TIS with a sucrose-free medium [51].…”