Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

Carlo, Anna De; Tarraf, Waed; Lambardi, Maurizio; Benelli̇, C.

doi:10.3390/agronomy11122414

Cited by 33 publications

(14 citation statements)

References 138 publications

(178 reference statements)

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“…Each of the three bioreactors contained 15 plants on MS medium without agar [9][10]. Also, 45 test tube plants were used as control samples.…”

Section: Methodsmentioning

confidence: 99%

Potato microclonal propagation method in a temporary immersion bioreactor

Sukhoparov,

Zakharenko,

Fedorova

2024

BIO Web Conf.

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A promising and efficient approach to obtaining a genetically pure and identical plant to a given explant is through microclonal propagation. This method pre-determines the reproduction of plants by managing, controlling, and regulating the parameters of external and internal conditions that influence the accelerated reproduction of cultivated plants. The main methods for implementing the accelerated reproduction of cultivated plants are propagation in a closed external environment using specialized nutrient solutions and the use of bioreactors with temporary immersion of plants. The process of reproducing cultivated plants, specifically potatoes, using temporary immersion bioreactors allows for the use of automated monitoring and control systems based on robust control systems. This increases productivity and ensures the quality of the reproduced explants. Research has led to the development of experienced statisticians who have established the impact of individual parameters on the management of plant reproduction on their quality indicators. The reliability of the study is ensured by a large sample size (over 200 objects), parametric statistics, and the use of group theory to estimate plant reproduction parameters.

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“…Each of the three bioreactors contained 15 plants on MS medium without agar [9][10]. Also, 45 test tube plants were used as control samples.…”

Section: Methodsmentioning

confidence: 99%

Potato microclonal propagation method in a temporary immersion bioreactor

Sukhoparov,

Zakharenko,

Fedorova

2024

BIO Web Conf.

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“…Ptak et al [154] demonstrated the positive influence of metatopolin and benzyladenine on plant development with two times more production of morphologically normal plants from SEs in TIS than SS medium without any hyperhydricity. TIS also helps reduce medium browning due to oxidation, plant contamination through air vents, and lower agitation stress on plant tissues due to the absence of mechanical devices [155,156]. Though TIS is mainly utilized for plant micropropagation, it also offers an alternative to SS and liquid culture for increasing biomass yield and secondary metabolite production at a low cost.…”

Section: Benefits and Drawbacks Of Tismentioning

confidence: 99%

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Nongdam

Beleski

Tikendra

et al. 2023

Plants

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Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation.

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“…Studies have indicated that it facilitates faster plant multiplication and is more effective for producing certain secondary metabolites compared to plants grown in soil [ 5 , 13 , 14 , 15 , 16 ]. The key aspect for maximizing the potential of in vitro cultures maintained in the PlantForm TIS is the experimental optimization of process parameters [ 4 , 5 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Assessment of Lignan Profiling and Biological Activities of Schisandra henryi Leaf and In Vitro PlantForm Bioreactor-Grown Culture Extracts

Jafernik,

Kubica,

Dziurka

et al. 2024

Pharmaceuticals

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This research’s scope encompassed biotechnological, phytochemical, and biological studies of Schisandra henryi, including investigations into its in vitro microshoot culture grown in PlantForm bioreactors (temporary immersion systems, TISs), as well as extracts from leaves of the parent plant, focusing on anti-inflammatory, antioxidant, anticancer, and antimicrobial activities. The phytochemical analysis included the isolation and quantification of 17 compounds from dibenzocyclooctadiene, aryltetralin lignans, and neolignans using centrifugal partition chromatography (CPC), HPLC-DAD, and UHPLC-MS/MS tandem mass spectrometry with triple quadrupole mass filter methods. Higher contents of compounds were found in microshoots extracts (max. 543.99 mg/100 g DW). The major compound was schisantherin B both in the extracts from microshoots and the leaves (390.16 and 361.24 mg/100 g DW, respectively). The results of the anti-inflammatory activity in terms of the inhibition of COX-1, COX-2, sPLA2, and LOX-15 enzymes indicated that PlantForm microshoot extracts showed strong activity against COX-1 and COX-2 (for 177 mg/mL the inhibition percentage was 76% and 66%, respectively). The antioxidant potential assessed using FRAP, CUPRAC, and DPPH assays showed that extracts from microshoot cultures had 5.6, 3.8, and 3.3 times higher power compared to extracts from the leaves of the parent plant, respectively. The total polyphenol content (TPC) was 4.1 times higher in extracts from the in vitro culture compared to the leaves. The antiproliferative activity against T-cell lymphoblast line Jurkat, breast adenocarcinoma cultures (MCF-7), colon adenocarcinoma (HT-29), and cervical adenocarcinoma (HeLa), showed that both extracts have considerable effects on the tested cell lines. The antimicrobial activity tested against strains of Gram-positive and Gram-negative bacteria and fungi showed the highest activity towards H. pylori (MIC and MBC 0.625 mg/mL).

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Temporary Immersion System for Production of Biomass and Bioactive Compounds from Medicinal Plants

Cited by 33 publications

References 138 publications

Potato microclonal propagation method in a temporary immersion bioreactor

Potato microclonal propagation method in a temporary immersion bioreactor

Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review

Comparative Assessment of Lignan Profiling and Biological Activities of Schisandra henryi Leaf and In Vitro PlantForm Bioreactor-Grown Culture Extracts

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