Biohydrogen Production from Pineapple Biomass Residue using Immobilized Co-culture of Clostridium sporogenes and Enterobacter aerogenes

Abstract

In this study, the co-culture bacteria of Clostridium sporogenes and Enterobacter aerogenes were immobilized onto two different support materials: loofah sponge and activated carbon (AC) sponge. Both immobilized co-cultures were used in the batch fermentation of pineapple residues for biohydrogen production. The performance of both immobilized loofah and AC sponge was compared with free cell (FC) co-culture in terms of biohydrogen cumulative production and production rate within 48 hr fermentation time. It was found that the immobilized co-culture on AC sponge produced the highest rate of biohydrogen of 35.9 mmol/hr/Lsubstrate compared to loofah and FC co-culture after 24 hr fermentation. However, in terms of preservation of biohydrogen production rate, loofah as a support showed better consistency in terms of performance for 48 hr fermentation time compared to AC. This study also showed that the pH of substrate has a relation to the optical density (OD600) reduction of the bacteria, which could affect biohydrogen production rate.

Recent Updates on the Conversion of Pineapple Waste (Ananas comosus) to Value-Added Products, Future Perspectives and Challenges

“…Biohydrogen produced from pineapple waste assisted with diluted acid pre-treatment at various substrate concentrations demonstrated that the highest biohydrogen produced was by using 26.4 g VS/L and 0.3 N sulfuric acid [24]. Abd Jalil et al [188] investigated the biohydrogen production from pineapple waste with co-culture bacteria that was immobilised onto loofah sponge and activated carbon. The highest biohydrogen produced from the pineapple waste with activated carbon was 35.9 mmol/hr/L substrate .…”

Advances and Applications of Clostridium Co-culture Systems in Biotechnology

“…In addition to the optimization of the fermentation conditions, cell immobilization is also considered a potential strategy for improving the stability of the Clostridium co-culture system as it can provide moderate conditions for microbial growth and minimize the effects of system inhibitors ( Laxman Pachapur et al, 2015 ). Jalil et al (2018) gained a biohydrogen production rate of 35.9 mmol/h/L substrate utilizing pineapple biomass residue by using an immobilized co-culture of C. sporogenes ATCC19404 and Enterobacter aerogenes ATCC13048. They found that using an activated carbon sponge provided a better support material compared to loofah sponge, as they could enhance the production rate by approximately 67% compared to the free cell co-culture ( Jalil et al, 2018 ).…”

“… Jalil et al (2018) gained a biohydrogen production rate of 35.9 mmol/h/L substrate utilizing pineapple biomass residue by using an immobilized co-culture of C. sporogenes ATCC19404 and Enterobacter aerogenes ATCC13048. They found that using an activated carbon sponge provided a better support material compared to loofah sponge, as they could enhance the production rate by approximately 67% compared to the free cell co-culture ( Jalil et al, 2018 ).…”