Advances and Applications of Clostridium Co-culture Systems in Biotechnology

Du, Yuanfen; Zou, Wei; Zhang, Kaizheng; Ye, Guangbin; Yang, Jian-Gang

doi:10.3389/fmicb.2020.560223

Cited by 65 publications

(45 citation statements)

References 125 publications

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“…Notably, its hydrolytic activity is often compared to that of commercial enzyme blends [137,138]. Moreover, in addition to ethanol production, members of the genus Clostridium are employed in a number of biorefinery applications, as widely reviewed in [139,140]. Regarding lignocellulosic biomass utilization, despite being able to degrade hemicellulose, C. thermocellum cannot utilize C5 sugars in its fermentative metabolism [134,141], which can also have an inhibiting effect [138].…”

Section: Thermophilic Fermentationmentioning

confidence: 99%

Biorefinery Gets Hot: Thermophilic Enzymes and Microorganisms for Second-Generation Bioethanol Production

et al. 2021

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To mitigate the current global energy and the environmental crisis, biofuels such as bioethanol have progressively gained attention from both scientific and industrial perspectives. However, at present, commercialized bioethanol is mainly derived from edible crops, thus raising serious concerns given its competition with feed production. For this reason, lignocellulosic biomasses (LCBs) have been recognized as important alternatives for bioethanol production. Because LCBs supply is sustainable, abundant, widespread, and cheap, LCBs-derived bioethanol currently represents one of the most viable solutions to meet the global demand for liquid fuel. However, the cost-effective conversion of LCBs into ethanol remains a challenge and its implementation has been hampered by several bottlenecks that must still be tackled. Among other factors related to the challenging and variable nature of LCBs, we highlight: (i) energy-demanding pretreatments, (ii) expensive hydrolytic enzyme blends, and (iii) the need for microorganisms that can ferment mixed sugars. In this regard, thermophiles represent valuable tools to overcome some of these limitations. Thus, the aim of this review is to provide an overview of the state-of-the-art technologies involved, such as the use of thermophilic enzymes and microorganisms in industrial-relevant conditions, and to propose possible means to implement thermophiles into second-generation ethanol biorefineries that are already in operation.

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Section: Thermophilic Fermentationmentioning

confidence: 99%

Biorefinery Gets Hot: Thermophilic Enzymes and Microorganisms for Second-Generation Bioethanol Production

et al. 2021

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“…For the calculation of growth-dependent product formation and substrate consumption, only the growth-related part, but not the cell lysis-related part of the biomass evolution rate from Equation ( 7) was taken into consideration in Equation (8). biomass growth * , ,…”

Section: Mathematical Modeling Approachmentioning

confidence: 99%

“…Nowadays, we await a comeback of the industrial ABE process as not only the demand for sustainable produced solvents and bulk chemicals is increasing, but butanol is further discussed as a promising biofuel. Beside the exploration of new non-food and inexpensive waste materials as substrates for the fermentation process [5,6], an important setscrew to improve the economics of the process is the development of new fermentation technologies [7,8]. These should allow effective raw material conversion with a high yield, accelerate the onset of Fuels 2021, 2 solvent formation in a biphasic ABE process and stabilize the bacteria in this metabolic phase.…”

Section: Introductionmentioning

confidence: 99%

Process Engineering of the Acetone-Ethanol-Butanol (ABE) Fermentation in a Linear and Feedback Loop Cascade of Continuous Stirred Tank Reactors: Experiments, Modeling and Optimization

Karstens

Trippel

Götz

2021

Fuels

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The production of butanol, acetone and ethanol by Clostridium acetobutylicum is a biphasic fermentation process. In the first phase the carbohydrate substrate is metabolized to acetic and butyric acid, in the following second phase the product spectrum is shifted towards the economically interesting solvents. Here we present a cascade of six continuous stirred tank reactors (CCSTR), which allows performing the time dependent metabolic phases of an acetone-butanol-ethanol (ABE) batch fermentation in a spatial domain. Experimental data of steady states under four operating conditions—with variations of the pH in the first bioreactor between 4.3 and 5.6 as well as the total dilution rate between 0.042 h−1 and 0.092 h−1—were used to optimize and validate a corresponding mathematical model. Beyond a residence time distribution representation and substrate, biomass and product kinetics this model also includes the differentiation of cells between the metabolic states. Model simulations predict a final product concentration of 8.2 g butanol L−1 and a productivity of 0.75 g butanol L−1 h−1 in the CCSTR operated at pHbr1 of 4.3 and D = 0.092 h−1, while 31% of the cells are differentiated to the solventogenic state. Aiming at an enrichment of solvent-producing cells, a feedback loop was introduced into the cascade, sending cells from a later state of the process (bioreactor 4) back to an early stage of the process (bioreactor 2). In agreement with the experimental observations, the model accurately predicted an increase in butanol formation rate in bioreactor stages 2 and 3, resulting in an overall butanol productivity of 0.76 g L−1 h−1 for the feedback loop cascade. The here presented CCSTR and the validated model will serve to investigate further ABE fermentation strategies for a controlled metabolic switch.

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“…Nowadays we await a comeback of the industrial ABE process as not only the demand for sustainable produced solvents and bulk chemicals is increasing, but butanol is further discussed as promising biofuel. Beside the exploration of new non-food and inexpensive waste materials as substrates for the fermentation process [5,6], an important setscrew to improve the economics of the process is the development of new fermentation technologies [7,8]. Those should allow effective raw material conversion with high yield, accelerate the onset of solvent formation in a biphasic ABE process and stabilize the bacteria in this metabolic phase.…”

Section: Introductionmentioning

confidence: 99%

Process Engineering of the Acetone-Ethanol-Butanol (ABE) Fermentation in a Linear and Feedback Loop Cascade of Continuous Stirred Tank Reactors: Experiments, Modeling and Optimization

Karstens¹,

Trippel²,

Götz³

2021

Preprint

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The production of butanol, acetone and ethanol by Clostridium acetobutylicum is a biphasic fer-mentation process. In the first phase the carbohydrate substrate is metabolized to acetic and bu-tyric acid, in the following second phase the product spectrum is shifted towards the economi-cally interesting solvents. Here we present a cascade of six continuous stirred tank reactors (CCSTR), which allows performing the time dependent metabolic phases of an ace-tone-butanol-ethanol (ABE) batch fermentation in a spatial domain. Experimental data of steady states under four operating conditions - with variations of the pH in the first bioreactor between 4.3 and 5.6 as well as the total dilution rate between 0.042 1/h and 0.092 1/h - were used to optimize and validate a corresponding mathematical model. Beyond a residence time distribution representation and substrate, biomass and product kinetics this model also includes the differen-tiation of cells between the metabolic states. Model simulations predict a final butanol product concentration of 8.2 g/L and a butanol productivity of 0.75 g/(L h) in the CCSTR operated at a pH in bioreactor 1 of 4.3 and D = 0.092 1/h, while 31 % of the cells are differentiated to the solventogenic state. Aiming at an enrichment of solvent-producing cells, a feedback loop was introduced into the cascade - sending cells from a later state of the process (bioreactor 4) back to an early stage of the process (bioreactor 2). In agreement with the experimental observations, the model accurately predicted an increase of butanol formation rate in bioreactor stages 2 and 3, resulting in an overall butanol productivity of 0.76 g/(L h) for the feedback loop cascade. The here presented CCSTR and the validated model will serve to investigate further ABE fermentation strategies for a controlled metabolic switch.

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Advances and Applications of Clostridium Co-culture Systems in Biotechnology

Cited by 65 publications

References 125 publications

Biorefinery Gets Hot: Thermophilic Enzymes and Microorganisms for Second-Generation Bioethanol Production

Biorefinery Gets Hot: Thermophilic Enzymes and Microorganisms for Second-Generation Bioethanol Production

Process Engineering of the Acetone-Ethanol-Butanol (ABE) Fermentation in a Linear and Feedback Loop Cascade of Continuous Stirred Tank Reactors: Experiments, Modeling and Optimization

Process Engineering of the Acetone-Ethanol-Butanol (ABE) Fermentation in a Linear and Feedback Loop Cascade of Continuous Stirred Tank Reactors: Experiments, Modeling and Optimization

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