Biofilm building capacity ofSalmonella entericastrains from the poultry farm environment

Abstract

The Biofilm (BF) building capacity of different serotypes of Salmonella enterica derived from the poultry farm environment was investigated. Starting point for the investigation was the question if farm‐isolated Salmonella serotypes with high importance for poultry meat and egg production are capable of forming a BF under defined laboratory conditions. Several isolates from different stages of the production cycle were chosen and compared to laboratory grown strains of the same serotype. BF building capacity was analyzed in a 96‐well format during a time period of 2 days. Pulse field gel electrophoresis was used to establish a relationship between different isolates. The BF building capacity of a monospecies BF was strongly dependent on the temperature used for incubation. Results indicated further that certain farm isolates were capable of forming BF under laboratory conditions, whereas laboratory grown strains were not. Considerable differences between different field serovars and within one serovar exist. In conclusion, the BF building capacity of poultry‐derived isolates is a function of adaptation to their host environment. Thus, the control of BF as a reservoir for Salmonella in the farm environment is of crucial importance for the overall improvement of food safety. Mechanical and substance‐based approaches for this control exist in several variations, but overall decontamination success is difficult to achieve and needs to be especially adapted to the farm environment.

Potentially Pathogenic Escherichia coli Can Form a Biofilm under Conditions Relevant to the Food Production Chain

“…Different biofilm-forming abilities between serotypes of Listeria monocytogenes (27,28) and between serovars of Salmonella (18,29) have also been reported. Little has previously been reported on possible differences in biofilm production between serotypes or serogroups of E. coli (30), although differences between strains have been published by several (31)(32)(33)(34).…”

Inhibition of quorum sensing in Chromobacterium violaceum by Syzygium cumini L. and Pimenta dioica L.

“…In Gram negative bacteria, QS is mediated through N-acyl homoserine lactones (AHL) [2] . Deeper understanding on QS has led to its role in controlling the production of virulence factors such as exopolysaccharide synthesis, biofilm formation, swarming motility, pigment production and antibiotic production in some pathogenic bacteria [3,4] . Owing to the importance of QS during bacterial pathogenesis, interfering with this mechanism is being considered as a rational strategy to attenuate their virulence [5] .…”

Salmonella spp. in poultry: a constant challenge and new insights

“…Differences in the ability to produce the BF are established among different serovars, or strains of the same Salmonella serovar (Schonewille et al, 2012). However, in vitro conditions used in research on BF formation capacities, may not always reflect the conditions required for BF formation in the environment.…”

“…These biofilms allow the persistence of Salmonella spp. in feed and food factory environments for months, and even years (Vestby et al, 2009;Schonewille et al, 2012;Prunić et al, 2016).…”