Inhibition of quorum sensing in Chromobacterium violaceum by Syzygium cumini L. and Pimenta dioica L.

Vasavi, Halkare Suryanarayana; Arun, A. B.; Rekha, P. D.

doi:10.1016/s2221-1691(13)60185-9

Cited by 44 publications

(23 citation statements)

References 25 publications

(31 reference statements)

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“…Another study by Huber et al (2003) showed anti quorum sensing effects of isolated phenolic compounds such as epigallocatechin gallate (EGCG), gallic acid and tannic acid on the bioluminescence of the quorum sensing biosensor E. coli MT102 pSB403 and the fluorescence of Pseudomonas putida pKR-C12, as well as biofilm formation and swarming motility in Burkholderia cepacia. In another study, the production of violacein was completely abolished by leaves of Centella asiatica L. and Adenanthera pavonina L, and the role of flavonoids on this action was suggested (Vasavi et al, 2013).…”

Section: Discussionmentioning

confidence: 94%

“…In addition to the above mentioned studies, medicinal plants have also been searched for quorum sensing inhibition against violacein production for extracts of Syzygiumcumini L. and Pimenta dioica L (Vasavi et al, 2013) and bioluminescence inhibition in V. harveyi for extracts from Rhizophora annamalayanacasca (Musthafa et al, 2013). Seeds from Cuminum cyminum, popularly known as cumin, presented as potent inhibitors of quorum sensing in C. violaceum, with inhibition reaching 86% with no measurable interference on bacterial growth (Packiavathy et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Anti-quorum sensing activity of phenolic extract from Eugenia brasiliensis (Brazilian cherry)

et al. 2016

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The aim of this study was to assess the anti-quorum sensing activity of phenolic extracts from grumixama (Eugenia brasiliensis), also known as Brazilian cherry, in concentrations that did not interfere with bacterial growth. The pulp phenolic compounds were extracted by using solid phage extraction in a mini-collumn C18 and quantified by spectrophotometry. The anti-quorum sensing activity was evaluated by testing the inhibition of violacein production in Chromobacterium violaceum and by evaluating the swarming motility in Aeromonas hydrophila and Serratia marcescens, both phenotypes regulated by quorum sensing. The phenolic extract strongly inhibited the production of violacein in C. violaceum, reducing its production in comparison with a control with no extract. No inhibition of growth was observed at the concentrations tested for quorum sensing inhibition. Confirming the quorum sensing inhibition phenotype, the extract was also able to inhibit swarming motility in S. marcescens and in A. hydrophila, although in the later the effect was marginal. Dverall, these results indicate that phenolic extract from E. brasiliensis presents quorum sensing inhibitory activity most likely due to the presence of fruit phenolics which have been implicated as quorum sensing inhibitors in Gram negative bacteria.Keywords: quorum sensing; anti-quorum sensing; phenolic extract; Brazilian cherry; Eugenia brasiliensis. Practical Application:The phenolic extract in this study presents potential applications in the food and pharmaceutical industries due to inhibition of phenotypes regulated by quorum sensing, which include production of virulence factors and spoilage enzymes. By inhibiting phenotypes associated with quorum sensing, the extract presents potential application as a natural preservative for food conservation and as an alternative or an adjuvant with antibiotics.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Anti-quorum sensing activity of phenolic extract from Eugenia brasiliensis (Brazilian cherry)

et al. 2016

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“…In a qualitative study, Shukla and Bhathena (2014) [ 50 ] have reported that several tannin-rich crude extracts show a broad spectrum of anti-QS activity. Another qualitative study on anti-AHL activity of pericarp of Punica granatum and an ethyl acetate fraction of S. cumini leaves has also been reported [ 22 , 51 ]. Hasan et al (2012) [ 23 ] have reported that P. emblica fruit extract can suppress quorum sensing genes in S. mutans .…”

Section: Resultsmentioning

confidence: 99%

Broad Spectrum Anti-Quorum Sensing Activity of Tannin-Rich Crude Extracts of Indian Medicinal Plants

Shukla¹,

Bhathena

2016

Scientifica

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Quorum sensing (QS) mechanisms have been demonstrated to have significance in expression of pathogenicity in infectious bacteria. In Gram negative bacteria the autoinducer molecules that mediate QS are acyl homoserine lactones (AHL) and in Gram positive bacteria they are peptides called autoinducing peptides (AIP). A screening of tannin-rich medicinal plants was attempted to identify extracts that could interrupt the QS mechanisms in both Gram positive and Gram negative bacteria over a wide range of concentrations and therefore potentially be potent agents that could act as broad spectrum QS inhibitors. Six out of the twelve Indian medicinal plant extracts that were analyzed exhibited anti-QS activity in Chromobacterium violaceum 12472 and in S. aureus strain with agr:blaZ fusion over a broad range of subinhibitory concentrations, indicating that the extracts contain high concentration of molecules that can interfere with the QS mechanisms mediated by AHL as well as AIP.

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“…Crude extracts of many plant parts were shown to possess anti-QS activity using C. violaceum as a model bacterium [19], [20], [21]. Crude plant extracts are often found to be more effective than isolated constituents at an equivalent dose perhaps owing to positive interactions between components of whole plant extracts.…”

Section: Introductionmentioning

confidence: 99%

Guava Leaf Extract Inhibits Quorum-Sensing and Chromobacterium violaceum Induced Lysis of Human Hepatoma Cells: Whole Transcriptome Analysis Reveals Differential Gene Expression

et al. 2014

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Quorum sensing (QS) is a process mediated via small molecules termed autoinducers (AI) that allow bacteria to respond and adjust according to the cell population density by altering the expression of multitudinous genes. Since QS governs numerous bioprocesses in bacteria, including virulence, its inhibition promises to be an ideal target for the development of novel therapeutics. We found that the aqueous leaf extract of Psidium guajava (GLE) exhibited anti-QS properties as evidenced by inhibition of violacein production in Chromobacterium violaceum and swarming motility of Pseudomonas aeruginosa. The gram-negative bacterium, C. violaceum is a rare pathogen with high mortality rate. In this study, perhaps for the first time, we identified the target genes of GLE in C. violaceum MTCC 2656 by whole transcriptome analysis on Ion Torrent. Our data revealed that GLE significantly down-regulated 816 genes at least three fold, with p value≤0.01, which comprises 19% of the C. violaceum MTCC 2656 genome. These genes were distributed throughout the genome and were associated with virulence, motility and other cellular processes, many of which have been described as quorum regulated in C. violaceum and other gram negative bacteria. Interestingly, GLE did not affect the growth of the bacteria. However, consistent with the gene expression pattern, GLE treated C. violaceum cells were restrained from causing lysis of human hepatoma cell line, HepG2, indicating a positive relationship between the QS-regulated genes and pathogenicity. Overall, our study proposes GLE as a QS inhibitor (QSI) with the ability to attenuate virulence without affecting growth. To the best of our knowledge, this is the first report which provides with a plausible set of candidate genes regulated by the QS system in the neglected pathogen C. violaceum.

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Inhibition of quorum sensing in Chromobacterium violaceum by Syzygium cumini L. and Pimenta dioica L.

Abstract: Asian Pac J Trop Biomed 2013; 3(12): 954-959

Cited by 44 publications

References 25 publications

Anti-quorum sensing activity of phenolic extract from Eugenia brasiliensis (Brazilian cherry)

Anti-quorum sensing activity of phenolic extract from Eugenia brasiliensis (Brazilian cherry)

Broad Spectrum Anti-Quorum Sensing Activity of Tannin-Rich Crude Extracts of Indian Medicinal Plants

Guava Leaf Extract Inhibits Quorum-Sensing and Chromobacterium violaceum Induced Lysis of Human Hepatoma Cells: Whole Transcriptome Analysis Reveals Differential Gene Expression

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