Biofertilizers are sustainable alternatives to producers in order to reduce or replace the use of chemical fertilizers. Act directly or indirectly on all or parts of plants grown by providing nutrients and increasing its productivity. These formulations are comprised of easily accessible to the producer materials such as crop residues and use micro-organisms that act beneficially increasing the availability of nutrients to plants through biological processes. The Hortbio® is enriched aerobic bio-fertilizer developed by Embrapa Vegetables -DF. The aim of this study was to characterize the Hortbio® with regard to respect for their cultivable microbiota, indole acetic acid (IAA) concentration and biological contaminants, and to evaluate the effect of biofertilizer doses in different varieties of lettuce. Was used, for evaluation of microbiota, different biofertilizer production times, these being 0, 5, 10, 15, 20, 25 and 30 days of either preparation, they being Hortbio® 0 and 40 Hortbio® and inoculum EM (effective microorganisms). Was used culture media for the isolation of micro-organisms, these being R2A, Martin AC, and THSM MYGP for the isolation of total bacteria, total fungi, actinomycetes, Trichoderma spp. and yeast, respectively. The microorganisms were identified by DNA sequencing. For the determination of IAA concentration was used Hortbio® 0 in production times 10, 20 and 30 using the HPLC technique. For the valuation of biological contaminants, we evaluated the presence of Salmonella spp. and fecal coliforms in the product, as well as helminths and nematodes in five different biofertilizer. All those with 10 days of preparation. The effect of different doses of biofertilizer (0, 50, 100, 150, 200, 250 kg ha -1 N) of three types of lettuce (leaf, crisp head and cos lettuce) in a randomized trial conducted in pots. They evaluated different agronomic traits of lettuce, which are: the height (HP) and width of the plant (WP) of plants, fresh mass (FM), the average number of leaves (ANL) and the length (SL) and stem diameter (SD). Soil fertility was assessed by determining the Ca 2+ , Mg 2+ , K + , Na + , P, H+Al, cation exchange capacity (CEC), pH and soil organic matter (SOM). 217 microbial isolates were recovered, with 120 bacteria, 61 yeasts and 36 fungi. Among the found genera,