Biofabrication of 3D cell-encapsulated tubular constructs using dynamic optical projection stereolithography

Wadnap, Soham; Krishnamoorthy, Srikumar; Zhang, Zhengyi; Xu, Changxue

doi:10.1007/s10856-019-6239-5

Cited by 36 publications

(38 citation statements)

References 51 publications

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“…[ 60 ] In addition, it allows the fabrication of vascular grafts with adequate mechanical properties and complex geometries while maintaining cell viability. [ 61 ] Melchiorri and colleagues used CAD designs based on images of the recipient aorta to mimic the specific curvature of the recipient. In addition they demonstrated that vascular grafts printed by this technique can sustain patency and functionality for up to 6 months after implantation.…”

Section: Tissue Engineered Vascular Graftsmentioning

confidence: 99%

From Arteries to Capillaries: Approaches to Engineering Human Vasculature

Fleischer

Tavakol

Vunjak-Novakovic

2020

Adv Funct Materials

111

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From microscaled capillaries to millimeter‐sized vessels, human vasculature spans multiple scales and cell types. The convergence of bioengineering, materials science, and stem cell biology has enabled tissue engineers to recreate the structure and function of different hierarchical levels of the vascular tree. Engineering large‐scale vessels aims to replace damaged arteries, arterioles, and venules and their routine application in the clinic may become a reality in the near future. Strategies to engineer meso‐ and microvasculature are extensively explored to generate models for studying vascular biology, drug transport, and disease progression as well as for vascularizing engineered tissues for regenerative medicine. However, bioengineering tissues for transplantation has failed to result in clinical translation due to the lack of proper integrated vasculature for effective oxygen and nutrient delivery. The development of strategies to generate multiscale vascular networks and their direct anastomosis to host vasculature would greatly benefit this formidable goal. In this review, design considerations and technologies for engineering millimeter‐, meso‐, and microscale vessels are discussed. Examples of recent state‐of‐the‐art strategies to engineer multiscale vasculature are also provided. Finally, key challenges limiting the translation of vascularized tissues are identified and perspectives on future directions for exploration are presented.

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Section: Tissue Engineered Vascular Graftsmentioning

confidence: 99%

From Arteries to Capillaries: Approaches to Engineering Human Vasculature

Fleischer

Tavakol

Vunjak-Novakovic

2020

Adv Funct Materials

111

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“…In this technique, a photopolymer will be cured and harden according to the 3D designed model using a light source, where the viscosity of raw materials can affect the final structure. 45,52 Limitations in the number of suitable materials, restriction in geometric fidelity, and dependency of accuracy to laser beam diameter are the observed challenge in this method. 53 FDM technology (Figure 1(c)) is a low cost, simple, high speed, and commonly used method to fabricate thermoplastic-based scaffolds.…”

Section: Bioprinting As An Innovative Technology For Bone Regenerationmentioning

confidence: 99%

“…3D printing is a versatile and accurate method that generates 3D structures through layer‐by‐layer printing the 3D model, which is provided by CAD or medical imaging. There are different methods for fabrication of acellular 3D structures, including solid freeform fabrication (SFF), 44 stereolithography (SLA), 45 fused deposition modeling (FDM), 46 powder‐fusion printing (PFP), 47 and laminated object manufacturing 48 (Figure 1). The selection of a suitable technique relies on the raw materials, processing speed, resolution, costs, and performance of final products 49 .…”

Section: Bioprinting As An Innovative Technology For Bone Regenerationmentioning

confidence: 99%

“…SLA technique (Figure 1(b)) is another subset of 3D printing technology techniques to create tissue engineering scaffolds due to the applicability of biologically relevant photopolymers. In this technique, a photopolymer will be cured and harden according to the 3D designed model using a light source, where the viscosity of raw materials can affect the final structure 45,52 . Limitations in the number of suitable materials, restriction in geometric fidelity, and dependency of accuracy to laser beam diameter are the observed challenge in this method 53 .…”

Section: Bioprinting As An Innovative Technology For Bone Regenerationmentioning

confidence: 99%

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Bioprinting a cell‐laden matrix for bone regeneration: A focused review

Ghorbani

Zhong

et al. 2020

J of Applied Polymer Sci

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Although many efforts have been made to regenerate the bone lesions, existing challenges can be mitigated through the development of tissue engineering scaffolds. However, the weak control on the microstructure of constructs, limitation in preparation of patient-specific and multilayered scaffolds, restriction in the fabrication of cell-laden matrixes, and challenges in preserving the drug/growth factors' efficacy in conventional methods have led to the development of bioprinting technology for regeneration of bone defects. So in this review, conventional 3D printers are classified, then the priority of the different types of bioprinting technologies for the preparation of the cell/growth factor-laden matrixes are focused. Besides, the bio-ink compositions, including polymeric/hybrid hydrogels and cell-based bio-inks are classified according to fundamental and recent studies. Herein, different effective parameters, such as viscosity, rheological properties, cross-linking methods, biodegradation biocompatibility, are considered. Finally, different types of cells and growth factors that can encapsulate in the bio-inks to promote bone repair are discussed, and both in vitro and in vivo achievement are considered. This review provides current and future perspectives of cell-laden bioprinting technologies. The restrictions and challenges are identified, and proper strategies for the development of cell-laden matrixes and high-performance printable bio-inks are proposed.

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“…Chondrocytes encapsulated in a GelMA bioink and 3D printed via SLA showed chondrocyte differentiation after 14 days [214,327]. Finally, a dynamic optical projection SLA system was used to print a vascular network with encapsulated living cells [334].…”

Section: Biocompatibility Biodegradability and Bioactivitymentioning

confidence: 99%

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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Biofabrication of 3D cell-encapsulated tubular constructs using dynamic optical projection stereolithography

Cited by 36 publications

References 51 publications

From Arteries to Capillaries: Approaches to Engineering Human Vasculature

From Arteries to Capillaries: Approaches to Engineering Human Vasculature

Bioprinting a cell‐laden matrix for bone regeneration: A focused review

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

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